蛋白质翻译后修饰之间的互作关系及其协同调控机理

doi:10.13560/j.cnki.biotech.bull.1985.2023-0963

摘要/Abstract

摘要：

蛋白质的翻译后修饰在细胞信号转导过程中起核心调控作用。除了单一翻译后修饰的调控外，细胞中还存在由多个翻译后修饰共同调控一些关键的代谢或信号通路的分子机制。翻译后修饰是蛋白质之间的语言，通过不同修饰之间的相互作用和协同调控，把复杂的信号整合后传递到下游其他蛋白并形成信号网络，最终影响蛋白质的结构、亚细胞定位、相互作用以及生物学功能。本文概述磷酸化、泛素化和类泛素化三类重要的翻译后修饰在植物细胞信号通路中的串扰作用模式及协同调控机制。

关键词: 激素, 受体, 激酶, 连接酶, 泛素化, 转录因子

Abstract:

Post-translational modifications（PTMs）have key regulatory roles in cell signal transduction networks. In addition to be a single PTM, many proteins undergo the regulation of multiple different types of PTMs，which functions in an orchestrated manner to adapt external or internal environmental cues. Such PTM crosstalk affects protein interactions, stabilities and subcellular localizations, and ultimately acts as a signaling hub to control diverse biological processes. Although PTM crosstalk has been extensively studied in humans and animals, the investigation in plants is just emerging. In this review, we discuss recent advances in crosstalk among three of the most common PTMs in plants, including phosphorylation, ubiquitination, and sumoylation，furthermore we highlight how this crosstalk may be connected to downstream signaling outputs.

Key words: phytohormone, receptor, kinase, ligase, ubiquitination, transcription factor

陈艳梅. 蛋白质翻译后修饰之间的互作关系及其协同调控机理[J]. 生物技术通报, 2024, 40(2): 1-8.

CHEN Yan-mei. Crosstalk Between Different Post-translational Modifications and Its Regulatory Mechanisms in Plant Growth and Development[J]. Biotechnology Bulletin, 2024, 40(2): 1-8.

图/表 1

图1 “磷酸化-泛素化-类泛素化”之间的串扰调控机制 A：“磷酸化-泛素化-类泛素化”在同一个蛋白的不同氨基酸残基上发生修饰，不同修饰之间通过互相拮抗或促进的方式共同调控蛋白质的分子功能；B：“磷酸化-泛素化-类泛素化”三类修饰通过以不同蛋白或酶相互作为底物进行修饰，形成正调控或负反馈调控环而发挥作用；C：“磷酸化-泛素化”协同调控受体激酶信号通路。P：磷酸化；S：类泛素化；Ub：泛素化； PM：细胞质膜

Fig. 1 Crosstalk and interaction among “Phosphorylation-ubiquitination-sumoylation” A: “Phosphorylation-ubiquitination-sumoylation” function at different amino acid residues of the same protein. The molecular function of different modifications among proteins is jointly regulated through mutual antagonism or promotion. B: “Phosphorylation-ubiquitination-sumoylation” are the modifications conducted through the use of different proteins or enzymes as substrates. C: The signaling processes of receptor-like kinase are co-regulated by “phosphorylation-ubiquitination”. P: Phosphorylation; S: sumoylation; Ub: ubiquitination; PM: plasma membrane

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