生物技术通报 ›› 2013, Vol. 0 ›› Issue (12): 21-26.
杨志刚, 郭子好, 姚琴琴, 成永旭
收稿日期:
2013-05-29
出版日期:
2013-12-20
发布日期:
2013-12-20
作者简介:
杨志刚,男,博士,副教授,研究方向:脂类分子营养;E-mail:zgyang@shou.edu.cn
基金资助:
Yang Zhigang, Guo Zihao, Yao Qinqin, Cheng Yongxu
Received:
2013-05-29
Published:
2013-12-20
Online:
2013-12-20
摘要: 多不饱和脂肪酸(PUFAs)对人体有重要的生理功能,脂肪酸去饱和酶是PUFAs合成途径中的关键酶,它控制着PUFAs的不饱和程度。近年来,随着对脂肪酸去饱和酶基因的研究,脂肪酸去饱和酶的研究得到了快速发展。从藻类、真菌、植物和动物等方面简述脂肪酸去饱和酶基因,特别是Δ9和Δ6脂肪酸去饱和酶基因方面的研究近况。
杨志刚, 郭子好, 姚琴琴, 成永旭. 脂肪酸去饱和酶基因的研究进展[J]. 生物技术通报, 2013, 0(12): 21-26.
Yang Zhigang, Guo Zihao, Yao Qinqin, Cheng Yongxu. Research Advance in Fatty Acid Desaturase Genes[J]. Biotechnology Bulletin, 2013, 0(12): 21-26.
[1] Kang JX. The importance of omega-6/omega-3 fatty acid ratio in cell function.The gene transfer of omega-3 fatty acid desaturase[J]. World Rev Nutr Diet, 2003, 92:23-36. [2] Lesf A, Kang JX, Xiao YF, et al. Clinical prevention of sudden cardiac death by n-3 polyunsaturated fatty acids and mechanism of prevention of arrhythmias by n-3 fish oils[J]. Circulation, 2003, 107:2646-2652. [3] 江黎明, 梁念慈. 脂肪酸抗肿瘤作用研究进展[J].湛江医学院学报, 1993, 11(1-2):46-50. [4] 李明春, 孙颖, 张琦, 等. 高山被孢霉Δ6脂肪酸脱氢酶基因在毕赤酵母中的胞内表达[J].生物工程学报, 2004, 20(1):34-38. [5] Pereira SL, Leonard AE, Mukerji P. Recent advances in the study of fatty acid desaturases from animals and lower eukaryotes[J]. Prostaglandins Leukot Essent Fatty Acids, 2003, 68(2):97-106. [6] Hsiao TY, Holmes B, Blanch HW. Identification and functional analysis of a delta-6 desaturase from the marine microalga Glossomastix chrysoplasta [J]. Mar Biotechno, 2007, 9(2):154-165. [7] Wada H, Murata N. Temperature-induced changes in the fatty acid composition of the cyanobacterium, Symchocystis PCC6803[J]. Plant Physiol, 1990, 92:1062-1069. [8] Reddy AS. Isolation of a Δ-6 desaturase gene from the cyanobacterium Synechocystis sp. strain PCC 6803 by gain-of-function exprssion in Anabanea sp. strain PCC7120[J]. P1ant Mol Bio, 1993, 27:293-300. [9] Nishizawa OI, Fujii T, Azuma M, et al. Low-temperature resistance of higher plants is significantly enhanced by a nonspecific cyanobacterial desaturase[J]. Nat Biotechnol, 1996, 14(8):1003-1006. [10] Suga K, Honjoh K, Furuya N, et al. Two low-temper ature-induced Chlorella genes for Δ12 and omega-3 fatty acid desaturase(FAD):Isolation of Δ12 and omega-3 FAD cDNA clones expression of Δ12 fad in Saccharomyces cerevisiae and expression of omega-3 fad in Nicotiana tabacum [J]. Biosci Biotechnol Biochem, 2002, 66(6):1314-1327. [11] 许友卿, 郑一民, 丁兆坤. 合成高度不饱和脂肪酸△6去饱和酶研究的回顾与前瞻[J].饲料工业, 2008, 29(14):41-44. [12] Huang YS, Chaudhary S, Thurmond J, et al. Cloning of Δ12- and Δ5-desaturases from Mortierella alpina and recombinant production of γ-linolenic acid in Saccharomyces cerevisiae [J]. Lipids, 1999, 34:649-659. [13] Huang YS, Chaudhary S, Thurmond JM, et al. Cloning of Δ12-and Δ6-desaturases from Mortierella alpina and recombinant production of gamma-linolenic acid in Saccharomyces cerevisiae [J].Lipids, 1999, 34(7):649-659. [14] Laoteng K, Mannontarat R, Tanticharoen M. Δ6-desaturases of Mucor rouxii with high similarity to plant Δ6-desaturases and its heterologous expression in Saccharomyces cerevisiae [J]. Bichem Biophys Res Commun, 2000, 279(1):17-22. [15] Maali-Amiri R, Goldenkova P, Yurevano, et al. Lipid fatty acid composition of Potato plants transformed with the Δ12-desaturase gene from Cyanobacterium [J].Russian Journal of Plant Physiology, 2007, 54(5):600-606. [16] Hao L, Mei XH, Zhao F, et al. Expression of Mucor circinelloides gene for Δ6 desaturase results in the accumulation of high levels of linolenic acid in transgenic tobacco[J]. Russian Journal of Plant Physiology, 2008, 55(1):77-81. [17] Somerville C, Browse J. Dissecting desaturation:plants prove advantageous[J].Trends Cell Biol Nature Biotechnology, 1996, 14:1003-1006. [18] Arondel V, Lemieux B, Hwang I, et al. Map-based cloning of a gene controllingomega-3 fatty acid desaturation in Arabidopsis [J]. Science, 1992, 258:1353-1355. [19] Okuley J, Lightner J, Feldmann K, et al. Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis[J]. Plant Cell, 1994, 6:147-158. [20] Iba K, Gibson S, Nishiuchi T, et al. A gene encoding a chloroplast omega-3 fatty acid desaturase complementsalterations in fatty acid desaturation and chloroplastcopy number of the fad7 mutant of Arabidopsis thaliana [J]. J Biol Chem, 1993, 268:24099-24105. [21] Gibson S, Arondel V, Iba K, et al. Cloning of a temperature-regulated gene encoding a chloroplast omega-3 desaturase from Arabidopsis thaliana [J]. Plant Physiol, 1994, 106:1615-1621. [22] Sayanova O. Expression of a borage desaturase cDNA containing go N-terminal cytochrome b 5 domain results in the accumulation of high levels of n-6 desaturated fatty acids in transgenic tobacco[J]. Proc Nail Acad Sci, 1997, 94:4211-4216. [23] Wang JW, Ming F, Pittman J, et al. Characterization of a rice(Oryza sativa L.)gene encoding a temperature-dependent chloroplast ω-3 fatty acid desaturase[J]. Bichem Biophys Res Commun, 2002, 340(4):1209-1216. [24] Mikkilineni V, Rocheford TR. Sequence variation and genomic organization of fatty acid desaturase-2(fad2)and fatty acid desaturase-6(fad6)cDNAs in maize[J]. Theor Appl Genet, 2003, 106(7):1326-1332. [25] Banilas G, Moressis A, Nikoloudakis N, et al. Spatial and temporal expressions of two distinct oleatedesaturases from olive(Olea europaea L. ). Plant Sci, 2005, 168(2):547-555. [26] Teixeira MC, Coelho N, Olsson ME, et al. Molecular cloning and expressionanalysis of three omega-6 desaturase genes from purslane(Portulaca oleracea L.)[J]. Biotechnol Lett, 2009, 31(7):1089-1101. [27] Dyer JM, Chapital DC. Production of linolenic acid in yeast cells expressing an omega-3 desaturase from tung(Aleurites fordii )[J].Am Oil Chem Soc, 2004, 81(7):647-651. [28] Zhou XR, Robert S, Singh S, et al. Heterologous production of GLA and SDA by expression of an Echium plantagineum Δ6 desaturase gene[J]. Plant Science, 2006, 170(3):665-673. [29] Napier JA, Hey SJ, Lacey DJ, et al. Identification of a Caenorhabdi-tis elegans Δ6 fatty acid-desaturase by heterologous expression in Saccharomyces cereviciae [J]. Biochem, 1998, 330:611-614. [30] Michaelson LV, Napier JA, Lewis M, et al. Functional identification of a fatty acid Δ5 desaturase gene from Caenorhabditis elegans [J]. FEBS Lett, 1998, 439:215-218. [31] Aki T, Shimada Y, Inagaki K, et al. Molecular cloning and functional characterisationof rat Δ6 fatty acid desaturase[J]. Biochem Biophys Res Commun, 1999, 255:575-579. [32] Cho HP, Nakamura MT, Clarke SD. Cloning expression and nutritional regulation of the human Δ6 desaturase[J]. Biol Chem, 1999, 274:471-477. [33] Leonard, AE, Kelder B, Bobik EG, et al. cDNA cloning and characterisation of human Δ5 desaturase involved in the synthesis of arachidonic acid[J]. Biochem, 2000, 347:719-724. [34] Hastings N, Agaba M, Tocher DR, et al.A vertebrate fatty acid desaturase with Δ5 and Δ6 activities[J]. Proc Natl Acad Sci USA, 2001, 98:14304-14309. [35] Alonso DL, Maroto FG, Ruiz JR, et al. Evolution of the membrane-bound fatty acid desaturases.[J]. Biochem Syst Ecol, 2003, 31(10):1111-1124. [36] Hastings N, Agaba MK, Tocher DR, et al. Molecular cloning and functional characterization of fatty acyl desaturase and elongase cDNAs involved in the production of eicosapentaenoic and docosahexaenoic acids from a-linolenic acid in Atlantic salmon(Salmo salar )[J]. Mar Biotechnol, 2004, 6(5):463-474. [37] Seiliez I, Panserat S, Kaushik S, et al. Cloning, tissue distribution and nutritional regulation of a Δ6-desaturase-like enzyme in rainbow trout[J]. Comp Biochem Physiol, 2001, 130:83- 93. [38] Seiliez I, Panserat S, Corraze G, et al. Cloning and nutritional regulation of a Δ6-desaturase-like enzyme in the marine teleost gilthead sea bream(Sparus aurata )[J]. Comp Biochem Physiol B, 2003, 135:449-460. [39] Zheng X, Seiliez I, Hastings N, et al. Characterization and comparison of fatty acyl Δ6 desaturase cDNAs from freshwater and marine teleost fish species[J]. Comparative Biochemistry and Physiology, 2004, 139:269-279. [40] Tocher DR, Zheng X, Schlechtriem C, et al. Highly unsaturated fatty acid synthesis in marine fish:cloning, functional characterization, and nutritional regulation of fatty acyl Δ6 desaturase of Atlantic cod(Gadus morhua L. )[J]. Lipids, 2006, 41:1003-1016. [41] 胡长博. 黄斑蓝子鱼△6脂肪酸去饱和酶cDNA的克隆及环境盐度、饲料脂类成分对其mRNA表达的影响[D].汕头大学, 2007. [42] González-Rovira A, Mourente G, Zheng X, et al. Molecular and functional characterization and expression analysis of a Δ6 fatty acyl desaturase cDNA of European Sea Bass(Dicentrarchus labrax L.)[J]. Aquaculture, 2009, 298:90-100. [43] 郁颖, 梁旭方, 李观贵, 等. 日本鳗鲡脂肪酸去饱和酶和延长酶基因的克隆与分析[J].水生态学杂志, 2009(5):56-62. [44] 李观贵, 梁旭方, 何珊, 等. 斜带石斑鱼脂肪酸去饱和酶和延长酶基因全长cDNA序列的克隆与分析[J]. 热带海洋学报, 2009, 28(6):95-102. [45] 许友卿, 郑一民, 丁兆坤. 军曹鱼Δ6 脂肪酸去饱和酶的cDNA 序列克隆与基因表达[J]. 中国水产科学, 2010, 17(6):1183-1191. [46] Ren H, Yu H, Xu P, et al. Influence of dietary fatty acids on muscle fatty acid composition and expression levels of Δ6 desaturase-like and Elovl5-like elongase in common carp(Cyprinus carpio var. Jian)[J].Comp Biochem Physiol B Biochem Mol Biol, 2012, 163:184-192. [47] Yang Z, Guo ZH, Ji LY, et al. Cloning and tissue distribution of a fatty acyl Δ6-desaturase-like gene and effects of dietary lipid levels on its expression in the hepatopancreas of Chinese mitten crab(Eriocheir sinensis )[J]. Comp Biochem Physiol B Biochem Mol Biol, 2013, 165:99-105. [48] Chang BE, Hsieh SL, Kuo CM. Molecular cloning of full-length cDNA encoding delta-9 desaturase through PCR strategies and its genomic organization and expression in grass carp(Ctenopharyng-odon idella )[J]. Mol Reprod Dev, 2001, 58:245-254. [49] Hsieha SL, Liao WL, Kuo CM. Molecular cloning and sequence analysis of stearoyl-CoA desaturase in milkfish, Chanos chanos [J]. Comp Biochem Physiol B Biochem Mol Biol, 2001, 130:467-477. [50] Polley SD, Tiku PE, Trueman RT, et al. Differential expression of cold- and diet-specific genes encoding two carp liver Δ9-acyl-CoA desaturase isoforms[J]. Am J Physiol Regul Integr Comp Physiol, 2003, 284:41-50. [51] Hsieh SL, Chang HT, Wu CH, et al. Cloning, tissue distribution and hormonal regulation of stearoyl-CoA desaturase in tilapia, Oreochromis mossambicus [J]. Aquaculture, 2004, 230:527-546. [52] Eigenheer AL, Young S, Blomquist GJ, et al. Isolation and molecular characterization of Musca domestica delta-9 desaturase sequences[J]. Insect Mol Biol, 2002, 11:533-542. [53] Rodriguez S, Hao G, Liu W, et al. Expression and evolution of Δ9 and Δ11 desaturase genes in the moth Spodoptera littoralis [J]. Insect Biochem Mol Biol, 2004, 34:1315-1328. [54] Guo ZH, Yang Z, Cheng Y, et al. Molecular characterization, tissue expression of acyl-CoA ?9-desaturase-like gene, and effects of dietary lipid levels on its expression in the hepatopancreas of the Chinese mitten crab(Eriocheir sinensis )[J]. Aquaculture, 2013, 402-403:58-65. |
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