NF-κB 信号通路在鱼类先天性免疫中的作用

摘要/Abstract

摘要： NF-κB(nuclear factor κB) 是一种广泛存在的核转录因子。经不同刺激信号激活后，参与多种免疫反应相关基因的#br#表达调控，对鱼类先天性免疫调节起着十分重要的作用。对鱼类NF-κB 的结构、功能及其信号传导途径进行概述，并对NF-κB 信#br#号通路在鱼类先天性免疫调节中的作用进行综述。

关键词: NF-κB 信号通路, 鱼类, 先天性免疫

Abstract: NF-κB(Nuclear factor κB)as an ubiquitously expressed nuclear transcription factor, involves in regulating the expression of immune-related genes after activation by a variety of stimuli and thus plays a very important role in the innate immune system of fish. It reviewed the structure, function and the signal pathway of fish NF-κB. Furthermore, we reviewed the role of NF-κB signal pathway in the innate immune system in fish.

Key words: NF-κB signal pathway, Fish, Innate immune system

杨冰贞,张民,王克坚. NF-κB 信号通路在鱼类先天性免疫中的作用[J]. 生物技术通报, 2014, 0(1): 46-52.

Yang Bingzhen, Zhang Min, Wang Kejian. Role of NF-κB Signal Pathway in the Innate Immune System of Fish[J]. Biotechnology Bulletin, 2014, 0(1): 46-52.

参考文献

[1] Hayden MS, West AP, Ghosh S. NF-κB and the immune response [J]. Oncogene, 2006, 25(51): 6758-6780.
[2] Gonzalez-Crespo S, Levine M. Related target enhancers for dorsal and NF-kappa B signaling pathways[J] (5156). Science, 1994, 264: 255-258.
[3] Ghosh S, Hayden MS. New regulators of NF-κB in inflammation[J].Nature Reviews Immunology, 2008, 8(11): 837-848.
[4] Suzuki K, Yamamoto T, Inoue JI. Molecular cloning of cDNA encoding the Xenopus homolog of mammalian RelB[J]. Nucleic Acids Research, 1995, 23(22): 4664-4669.
[5] Kao KR, Hopwood ND. Expression of a mRNA related to c-rel and dorsal in early Xenopus laevis embryos[J]. Proceedings of the National Academy of Sciences, 1991, 88(7): 2697-2701.
[6] Suzuki K, Tsuchida J, Yamamoto T, et al. Identification and expression of the Xenopus homolog of mammalian p100-NF- κB2[J]. Gene, 1998, 206(1): 1-9.
[7] Tannahill D, Wardle FC. Control of axis formation in Xenopus by the NF-kappa B/I kappa B system[J]. The International Journal of Developmental Biology, 1995, 39(4): 549.
[8] Naugler WE, Karin M. NF-κB and cancer-identifying targets and mechanisms[J]. Current Opinion in Genetics & Development,2008, 18(1): 19-26.
[9] Rutschmann S, Jung AC, Hetru C, et al. The Rel protein DIF mediates the antifungal but not the antibacterial host defense in Drosophila[J]. Immunity, 2000, 12(5): 569-580.
[10] Correa RG, Tergaonkar V, Ng JK, et al. Characterization of NFkappa B/I kappa B proteins in zebrafish and their involvement in notochord development[J]. Molecular and Cellular Biology,2004, 24(12): 5257-5268.
[11] Huxford T, Ghosh G. A structural guide to proteins of the NF- κB signaling module[J]. Cold Spring Harbor Perspectives in Biology, 2009, 1(3): a000075.
[12] Huxford T, Huang DB, Malek S, et al. The crystal structure of the I kappa B alpha / NF-kappa B complex reveals mechanisms of NFkappaB inactivation[J]. Cell, 1998, 95: 759-770.
[13] Caamano J, Hunter CA. NF-κB family of transcription factors: central regulators of innate and adaptive immune functions[J].Clinical Microbiology Reviews, 2002, 15(3): 414-429.
[14] Grilli M, Chiu JJS, Lenardo M. NF-κB and Rel: participants in a multiform transcriptional regulatory system[J]. International Review of Cytology, 1993, 143: 1-62.
[15] Elizabeth B. NF-κB and Rel proteins in innate immunity[J].Advances in Immunology, 1995, 58: 1.
[16] Hatada EN, Krappmann D, Scheidereit C. NF-κB and the innate immune response[J]. Current Opinion in Immunology, 2000,12: 52-58.
[17] Wang T, Zou J, Cunningham C, et al. Cloning and functional characterisation of the interleukin-1β1 promoter of rainbow trout (Oncorhynchus mykiss)[J]. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression, 2002, 1575(1): 108- 116.
[18] Nascimento DS, Vale A, Tomás AM, et al. Cloning, promoter analysis and expression in response to bacterial exposure of sea bass(Dicentrarchus labrax L.)interleukin-12 p40 and p35 subunits[J]. Molecular Immunology, 2007, 44(9): 2277- 2291.
[19] Lin B, Chen S, Cao Z, et al. Acute phase response in zebrafish upon Aeromonas salmonicida and Staphylococcus aureus infection: Striking similarities and obvious differences with mammals[J].Molecular Immunology, 2007, 44(4): 295-301.
[20] Collet B, Boudinot P, Benmansour A, et al. An Mx1 promoter? reporter system to study interferon pathways in rainbow trout[J].Developmental & Comparative Immunology, 2004, 28(7): 793- 801.
[21] Chen YM, Su YL, Lin JHY, et al. Cloning of an orange-spotted grouper(Epinephelus coioides)Mx cDNA and characterisation of its expression in response to nodavirus[J]. Fish & Shellfish Immunology, 2006, 20(1): 58-71.
[22] Moon JY, Nam BH, Kong HJ, et al. Maximal transcriptional activation of piscine soluble Toll-like receptor 5 by the NF-κB subunit p65 and flagellin[J]. Fish & Shellfish Immunology,2011, 31(6): 881-886.
[23] Su J, Jang S, Yang C, et al. Genomic organization and expression analysis of Toll-like receptor 3 in grass carp(Ctenopharyngodon idella)[J]. Fish & Shellfish Immunology, 2009, 27(3): 433- 439.
[24] Dann SG, Ted Allison W, Veldhoen K, et al. Chromatin immunoprecipitation assay on the rainbow trout opsin proximal promoters illustrates binding of NF-κB and c-jun to the SWS1 promoter in the retina. Experimental eye research, 2004, 78: [J] (5) 1015-1024.
[25] Ghosh S, May MJ, Kopp EB. NF-kappaB and Rel proteins: evolutionarily conserved mediators of immune responses[J].Science Signaling, 1998, 16(1): 225.
[26] Kong HJ, Moon JH, Moon JY, et al. Cloning and functional characterization of the p65 subunit of NF-κB from olive flounder (Paralichthys olivaceus)[J]. Fish and Shellfish Immunology,2011, 30: 406-411.
[27] Yazawa R, Kondo H, Hirono I, et al. Cloning and characterization of the IκBα gene from Japanese flounder, Paralichthys olivaceu[J] s. Fish and Shellfish Immunology, 2007, 23: 808 -814.
[28] Wang L, Zhou ZH, Guo CJ, et al. The alpha inhibitor of NF-κB (IκBα)from the mandarin fish binds with p65 NF-κB. Fish and Shellfish Immunology, 2009, 26: 473-482.
[29] Sangrador-Vegas A, Smith TJ, Cairns, MT. Cloning and characterization of a homologue of the alpha inhibitor of NF-kappaB in Rainbow trout(Oncorhynchus mykiss)[J]. Veterinary Immunology and Immunopathology, 2005, 13: (1-2): 1-7.
[30] Yamamoto M, Takeda K. Role of nuclear IκB proteins in the regulation of host immune responses[J]. Journal of Infection and Chemotherapy, 2008, 14(4): 265-269.
[31] Zhang M, Xiao Z, Sun L. Overexpression of NF-κB inhibitor alpha in Cynoglossus semilaevis impairs pathogen-induced immune response[J]. Developmental & Comparative Immunology, 2012,36(1): 253-257.
[32] Roach JC, Glusman G, Rowen L, et al. The evolution of vertebrate Toll-like receptors[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(27): 9577- 9582.
[33] Jault C, Pichon L, Chluba J. Toll-like receptor gene family and TIR-domain adapters in Danio rerio[J]. Molecular Immunology,2004, 40(11): 759-771.
[34] Tsujita T, Ishii A, Tsukada H, et al. Fish soluble Toll-like receptor (TLR)5 amplifies human TLR5 response via physical binding to flagellin[J]. Vaccine, 2006, 24(12): 2193-2199.
[35] Bilodeau AL, Peterson BC, Bosworth BG. Response of toll-like receptors, lysozyme, and IGF-I in back-cross hybrid(F1 male (blue× hannel)× emale channel)catfish challenged with virulent Edwardsiella ictaluri[J]. Fish & Shellfish Immunology,2006, 20(1): 29-39.
[36] Peterson BC, Bosworth BG, Bilodeau AL. Differential gene expression of IGF-I, IGF-II, and toll-like receptors 3 and 5 during embryogenesis in hybrid(channel× lue)and channel catfish[J]. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2005, 141(1): 42-47.
[37] Doyle SE, Oconnell R, Vaidayasa SA, et al.Toll-like receptor 3 mediates a more potent antiviral resonse than toll-like receptor 4 [J]. Journal of Immunology, 2003, 170(7): 3565-3571.
[38] Kawai T, Akira S. TLR signaling[J]. Cell Death & Differentiation,2006, 13(5): 816-825.
[39] Jault C, Pichon L, Chluba J. Toll-like receptor gene family, TIRdomain adapters in Danio rerio[J]. Molecular Immunology,2004, 40: 759-771.
[40] Kawai T, Akira S. The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors[J]. Nature Immunology, 2010, 11(5): 373-384.
[41] O’Neill LAJ, Bowie AG. The family of five: TIR-domaincontaining adaptors in Toll-like receptor signalling[J]. Nature Reviews Immunology, 2007, 7(5): 353-364.
[42] Dong C, Davis RJ, Flavell RA. MAP kinases in the immune response[J]. Annual Review of Immunology, 2002, 20: 55-72.
[43] Takano T, Kondo H, Hirono I, et al. Identification and characterization of a myeloid differentiation factor 88(MyD88) cDNA and gene in Japanese flounder, Paralichthys olivaceus[J].Developmental and Comparative Immunology, 2006, 30: 807-816.
[44] Skjaeveland I, lliev DB, Strandskog G, et al. Identification and characterization of TLR8 and MyD88 homologs in Atlantic salmon (Salmon salar)[J]. Development and Comparative Immunology,2009, 33: 1011-1017.
[45] Oshiumi H, Matsuo A, Matsumoto M, et al. Pan-vertebrate toll-like receptors during evolution[J]. Current Genomics, 2008, 9: 488493.
[46] Fan S, Chen S, Liu Y, et al. Zebrafish TRIF, a Golgi-localized protein,participates in IFN induction and NF-kappaB activation[J].Journal of Immunology, 2008, 180: 5373-5383.
[47] Matsuo A, Oshiumi H, Tsujita T, et al. Teleost TLR22 recognizes RNA duplex to induce IFN and protect cells from birnaviruses[J].Journal of Immunology, 2008, 181(5): 3474-3485.
[48] 丁旭. 斜带石斑鱼Toll 样受体22 基因的cDNA 克隆, 模式分析及其信号通路的初步探讨[D]. 海口: 海南大学, 2012.
[49] Engeszer RE, Barbiano D, Alberici L, et al. Timing and plasticity of shoaling behavior in the zebrafish, Danio rerio[J]. Animal Behavior, 2007, 74(5): 1269-1275.
[50] Kagan JC, Medzhitov R. Phosphoinositide-mediated adaptor recruitment controls Toll-like receptor signaling[J]. Cell, 2006,125(5): 943-955.
[51] Kagan JC, Su T, Horng T, et al. TRAM couples endocytosis of Toll-like receptor 4 to the induction of interferon-β[J]. Nature Immunology, 2008, 9(4): 361-368.
[52] Saeij JPJ, Vries BJ, Wiegertjes GF. The immune response of carp to Trypanoplasma borreli: kinetics of immune gene expression and polyclonal lymphocyte activation[J]. Developmental & Comparative Immunology, 2003, 27(10): 859-874.
[53] Pan CY, Huang TC, Wang YD, et al. Oral administration of recombinant epinecidin-1 protected grouper(Epinephelus coioides) and zebrafish(Danio rerio)from Vibrio vulnificus infection and enhanced immune-related gene expressions[J]. Fish & Shellfish Immunology, 2012, 32(6): 947-957.
[54] Levraud JP, Boudinot P, Colin I, et al. Identification of the zebrafish IFN receptor: implications for the origin of the vertebrate IFN system[J]. Journal of Immunology, 2007, 178(7): 4385-4394.
[55] Wang WL, Liu W, Gong HY, et al. Activation of cytokine expression occurs through the TNFα/NF-κB-mediated pathway in birnavirusinfected cells[J]. Fish & Shellfish Immunology, 2011, 31: 1021.
[56] del Castillo CS, Hikima J, Ohtani M, et al. Characterization and functional analysis of two PKR genes in fugu(Takifugu rubripes)[J]. Fish & Shellfish Immunology, 2012, 32: 79-88.
[57] Bergan V, Steinsvik S, Xu H, et al. Promoters of type I interferon genes from Atlantic salmon contain two main regulatory regions [J]. FEBS Journal, 2006, 273(17): 3893-3906.
[58] Robertsen B. Expression of interferon and interferon-induced genes in salmonids in response to virus infection, interferon-inducing compounds and vaccination[J]. Fish & Shellfish Immunology,2008, 25(4): 351-357.
[59] Hong JR, Guan BJ, Her GM, et al. Aquatic birnavirus infection activates the transcription factor NF-kappaB via tyrosine kinase signalling leading to cell death[J]. Journal of Fish Diseases,2008, 31: 451-460. 通讯作者: 王克坚，男，博士，教授，研究方向: 海洋分子生物学与免疫毒理学；E-mail: wkjian@xmu.edu.cn