抗氧化系统参与不同抗性烟草品种幼苗对干旱和低温综合抗性的形成

生物技术通报 ›› 2014, Vol. 0 ›› Issue (1): 132-142.

抗氧化系统参与不同抗性烟草品种幼苗对干旱和低温综合抗性的形成

王莎莎¹,盛业龙¹,马文广²,郝大海¹,张建波¹,龚明¹

1. 云南师范大学生命科学学院生物能源持续开发利用教育部工程研究中心云南省生物质能与环境生物技术重点实验室，昆明 650500
2. 云南省烟草农业科学研究院，玉溪 653100

收稿日期:2013-09-29 出版日期:2014-01-23 发布日期:2014-01-23
作者简介:王莎莎，女，博士，研究方向: 植物逆境生理和分子生物学；E-mail: vanessa.shasha83@gmail.com ；盛业龙同为本文第一作者
基金资助:
国家自然科学基金项目(30460016)，中国烟草总公司云南省公司科技计划项目(2011YN10)

Involvement of Antioxidant Defense System in Drought and Chilling Comprehensive Resistance Formation in Different#br#Resistant Varieties of Tobacco Seedlings

Wang Shasha¹, Sheng Yelong¹, Ma Wenguang², Hao Dahai¹, Zhang Jianbo¹, Gong Ming¹

1. School of Life Sciences，Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province， Engineering Research Center of Sustainable Development and Utilization of Biomass Energy of Ministry of Education，Yunnan Normal University ，Kunming 650500；
2. Yunnan Academy of Tobacco Agricultural Sciences，Yuxi 653100

Received:2013-09-29 Published:2014-01-23 Online:2014-01-23

摘要/Abstract

摘要： 以对干旱和低温胁迫具不同抗性的两个烟草品种MSK326 和云烟203 为材料，研究了抗氧化系统在不同抗性烟草#br#品种幼苗对干旱和低温综合抗性形成中的作用。在干旱和低温胁迫下，MSK326 的存活率均显著高于云烟203，并具较低的叶片电#br#解质渗漏率和丙二醛含量，显示了较强的综合抗性。对细胞抗氧化剂和抗氧化酶的测定显示，在干旱和低温胁迫下，MSK326 幼苗#br#中还原型抗氧化剂AsA、GSH 含量及其在总抗氧化剂中的比例，以及重要的抗氧化酶SOD、CAT、POD、APX 的活性均高于云烟#br#203，并且在胁迫后期差异尤为明显。上述结果表明，MSK326 在胁迫下具较强的氧化还原能力，有助于减轻干旱和低温胁迫引发#br#的氧化损伤，抗氧化系统参与了两个抗性不同的品种对干旱和低温胁迫综合抗逆性的形成。

关键词: 抗氧化系统, 烟草, 干旱胁迫, 低温胁迫, 综合抗逆性

Abstract: Two varieties of tobacco with different drought/chilling-resistance, MSK326 and yunyan203, were taken as experimental materials in this study, aiming at understanding the involvement of antioxidant defense system in drought and chilling comprehensive resistance formation of tobacco seedlings. MSK326 had a higher viabillity than yunyan203, with a lower relative electrolytic leakage and MDA content of leaves under both drought and chilling stress, demonstrating a higher comprehensive resistance to drought and chilling stress than yunyan203. The results of cellular antioxidant contents and enzymatic activity determination showed that, under drought or chilling stress, MSK326 could retain higher contents of AsA and GSH, ratio of reduced to total antioxidants and activities of the antioxidant enzymes(SOD, CAT, POD,APX)than yunyan203, especially at the late stage of stress. All these results indicated that an enhanced intracellular anti-oxidative capacity of MSK326 could be helpful to alleviate stress-induced oxidative damages, indicating the involvement of antioxidant system in drought and chilling comprehensive resistance formation of tobacco seedlings.

Key words: Antioxidant defense system, Tobacco, Drought stress, Chilling stress, Comprehensive resistance

王莎莎,盛业龙,马文广,郝大海,张建波,龚明. 抗氧化系统参与不同抗性烟草品种幼苗对干旱和低温综合抗性的形成[J]. 生物技术通报, 2014, 0(1): 132-142.

Wang Shasha, Sheng Yelong, Ma Wenguang, Hao Dahai, Zhang Jianbo, Gong Ming. Involvement of Antioxidant Defense System in Drought and Chilling Comprehensive Resistance Formation in Different#br#Resistant Varieties of Tobacco Seedlings[J]. Biotechnology Bulletin, 2014, 0(1): 132-142.

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