三种霉菌产纤维素酶能力分析与培养条件优化

生物技术通报 ›› 2014, Vol. 0 ›› Issue (1): 182-187.

三种霉菌产纤维素酶能力分析与培养条件优化

胡翠英,李良智,赵建,钱玮,顾华杰

苏州科技学院化学生物与材料工程学院，苏州 215009

收稿日期:2013-10-14 出版日期:2014-01-23 发布日期:2014-01-23
作者简介:胡翠英，女，硕士，研究方向: 发酵工程研究；E-mail: 13151170848@163.com

Analysis on Cellulase Producing Abilities of Three Kinds of Fungi and Optimization of Cultivation Conditions

Hu Cuiying, Li Liangzhi, Zhao Jian, Qian Wei, Gu Huajie

College of Chemistry，Biology and Materials Engineering，Suzhou University of Science and Technology，Suzhou 215009

Received:2013-10-14 Published:2014-01-23 Online:2014-01-23

摘要/Abstract

摘要： 对实验室现有3 种真菌产纤维素酶能力的分析及培养条件优化。比较了3 种菌在刚果红培养基上的透明圈大小、#br#并分析产纤维素酶酶活；通过单因素与响应面分析的方法优化毛酶产纤维素酶的培养条件。通过试验得出3 种真菌均能产纤维素酶，#br#毛霉能产较多的纤维素酶。毛霉产纤维素酶的最佳条件为: pH 5.0，转速 220 r/min，发酵时间47 h，发酵温度 35 ℃，纤维素酶活为#br#6.99#br#U/mL。毛霉、青霉、曲霉均产纤维素酶，毛霉能降解玉米芯纤维素。

关键词: 霉菌, 纤维素酶, 培养条件, 优化

Abstract: We analyzed the cellulose degradation ability of three kinds of fungi (Penicillium, Mucor and Aspergillus) preserved in the laboratory and optimalized the culture conditions. The transparent ring size of three kinds of fungi training in the congo red medium and in the corncob congo red medium was observed. The cellulase activity was measured of the three fungi cultured in the CMC-Na medium. Single factor and response surface methodology were used to optimize the culture condition. The three kinds of fungi all could produce cellulase. The cellulase activity of Mucor was higher than the other two cultured in the the CMC-Na medium. The optimized conditions were below : pH was 5.0, the shaking speed was 220 r/min，fermentation time was 47 h，fermentation temperature was 35 ℃. The highest CMCase activity was 6.99 U/mL training on the optimized condition. Penicillium, Mucor and Aspergillus produced cellulase and Mucor could degrade corncob.

Key words: Fungi, Cellulase, Culture condition, Optimize

胡翠英,李良智,赵建,钱玮,顾华杰. 三种霉菌产纤维素酶能力分析与培养条件优化[J]. 生物技术通报, 2014, 0(1): 182-187.

Hu Cuiying, Li Liangzhi, Zhao Jian, Qian Wei, Gu Huajie. Analysis on Cellulase Producing Abilities of Three Kinds of Fungi and Optimization of Cultivation Conditions[J]. Biotechnology Bulletin, 2014, 0(1): 182-187.

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