高温短程反硝化菌Brevibacillus sp.XF-03 特性及其降解动力学

摘要/Abstract

摘要： 采用梯度驯化方法，使得菌株Brevibacillus sp.XF-03 在高温(50℃)条件下，能够降解1 000 mg/L 亚硝态氮，并#br#通过单因素试验对其生长碳源和C/N 进行优化，结果显示，菌株Brevibacillus sp.XF-03 短程反硝化最适碳源为琥珀酸钠，C/N 为#br#12∶1。在此最佳条件下，42 h 对初始浓度为100 mg/L 亚硝态氮去除率为95.1%。对该菌株亚硝态氮降解动力学过程进行模拟，符#br#合基质抑制型的 Haldane 模型，各参数分别为: 最大比降解速率(μmax)=1.28 h-1，半饱和常数(KS)=451.42 mg/L，底物抑制常#br#数#br#(Ki)=176.77 mg/L。初步探讨了亚硝酸盐还原酶(NIR)活性，在该菌株生长指数期的后期，亚硝酸盐还原酶比活力达0.279(U/#br#mg protein)。

关键词: Brevibacillus sp.XF-03, 高温, 短程反硝化, 亚硝态氮降解动力学, 酶活性

Abstract: The denitrifier Brevibacillus sp. XF-03 could reduce high nitrite concentration(1 000 mg/L)under the condition of high temperature(50℃)by the method of gradient domestication. The effects of carbon source and C/N on growth and denitrification of the denitrifier were optimized by single factor experiments. The results showed that the optimum shortcut denitrification carbon source of Brevibacillus sp. XF- 03 was sodium succinate, optimum C/N ratio 12∶1. Under the optimal condition, the nitrite removal efficiency reached 95.1% within 42 hours when the original concentration of NO- 2-N was 100 mg/L. Nitrite degradation kinetic studies indicated that the strain followed Haldane’s model,and the parameters were: μmax(maximum specific rate)=1.28 h-1, Ks(half-satruration constant)=451.42 mg/L, K i (inhibition constant)= 176.77 mg/L. Nitrite reductase(NIR)activity was preliminary discussed. The NIR specific activity reached 0.279(U/mg protein)at the lag phase of exponential growth.

Key words: Brevibacillus sp.XF-03, High temperature, Shortcut denitrification, Nitrite degradation kinetics, Enzyme activity

郝敏娜,杨云龙,葛启隆. 高温短程反硝化菌Brevibacillus sp.XF-03 特性及其降解动力学[J]. 生物技术通报, 2014, 0(1): 177-181.

Hao Minna, Yang Yunlong, Ge Qilong. Characteristics and Degradation Kinetics of Thermophilic Shotcut Denitrifier Brevibacillus sp.XF-03[J]. Biotechnology Bulletin, 2014, 0(1): 177-181.

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