一株西沙群岛野生诺尼内生细菌NG14的分类鉴定及拮抗活性

生物技术通报 ›› 2014, Vol. 0 ›› Issue (3): 100-105.

一株西沙群岛野生诺尼内生细菌NG14的分类鉴定及拮抗活性

刘洋, 李金霞, 姚粟, 张明娟, 陈建国, 程池

(中国食品发酵工业研究院中国工业微生物菌种保藏管理中心，北京 100015)

收稿日期:2013-09-06 出版日期:2014-03-29 发布日期:2014-03-31
作者简介:刘洋，男，博士，工程师，研究方向:微生物分子生物学与生态学；E-mail:ly81150@163.com
基金资助:
国家自然科学基金项目(31300008)，中国食品发酵工业研究院科技发展基金(博士基金)项目(2012KJFZ-BS-01)

Identification and Antagonistic Activity of Endophytic Bacterial Strain NG14 Isolated from the Fruits of Paracel Islands Noni(Morinda citrifolia L.)

Liu Yang, Li Jingxia, Yao Su, Zhang Mingjuan, Chen Jianguo, Cheng Chi

(China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries, Beijing 100015)

Received:2013-09-06 Published:2014-03-29 Online:2014-03-31

摘要/Abstract

摘要： 植物的内生菌与植物生长健康及其功效性成分产生具有一定的相关性。以分离自我国西沙群岛野生诺尼成熟果实的内生细菌菌株NG14为对象，利用形态学观察，生理生化特征鉴定及系统发育学分析，对其菌种的分类地位进行研究，将其鉴定为多黏类芽孢杆菌(Paenibacillus polymyxa)；并通过选择多种植物及人体病原菌作为生防对象进行拮抗试验，发现了该菌株具有广谱且良好的拮抗病原菌活性。

关键词: 诺尼, 内生细菌, NG14, 鉴定, 拮抗活性

Abstract: Endophytes related with healthy and efficacy component production of plants. In this study，the bacterial strain NG14 isolated from Paracel Islands Noni(Morinda citrifolia L.)fruit. NG14 was identified as Paenibacillus polymyxa by polyphasic taxonomy basing on morphologicial and physiological methods，and 16S rRNA gene phylogenetic analysis. Through the antagonism test，results showed that the strain NG14 had a comprehensive and fine antagonistic activity against pathogens chosen for this research.

Key words: Noni, Endophytes, NG14, Identification, Antagonistic activity

刘洋, 李金霞, 姚粟, 张明娟, 陈建国, 程池. 一株西沙群岛野生诺尼内生细菌NG14的分类鉴定及拮抗活性^{[J]. 生物技术通报, 2014, 0(3): 100-105.}

Liu Yang, Li Jingxia, Yao Su, Zhang Mingjuan, Chen Jianguo, Cheng Chi. Identification and Antagonistic Activity of Endophytic Bacterial Strain NG14 Isolated from the Fruits of Paracel Islands Noni(Morinda citrifolia L.)[J]. Biotechnology Bulletin, 2014, 0(3): 100-105.

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