地木耳中脂溶性物质抑菌活性研究

生物技术通报 ›› 2014, Vol. 0 ›› Issue (4): 83-86.

地木耳中脂溶性物质抑菌活性研究

刁毅^1，2, 杨足君¹

(1.电子科技大学生命科学与技术学院，成都 610054；
2.攀枝花学院生物与化学工程学院干热河谷特色生物资源开发四川省高等学校重点实验室，攀枝花 617000)

收稿日期:2013-10-30 出版日期:2014-04-29 发布日期:2014-04-29
作者简介:刁毅，男，博士研究生，副教授，研究方向：植物抗病性；E-mail：diaoy163@163.com
基金资助:
攀枝花市科技局项目(2012CY-S-22(9))

Study on Antibacterial Effects of Fat-soluble Compositions in Nostoc commune

DiaoYi^1，2, Yang Zujun¹,

(1. School of Life Science and Technology，University of Electronic Science and Technology of China，Chengdu 610054；
2. College of Biological and Chemical Engineering，Sichuan Province Key Laboratory of Characteristic Biological Resources of Dry and Hot River Valley，Panzhihua University，Panzhihua 617000)

Received:2013-10-30 Published:2014-04-29 Online:2014-04-29

摘要/Abstract

摘要： 采用索氏提取法从地木耳中提取脂溶性物质，经硅胶柱层析将脂溶性物质分离成石油醚洗脱组分(非极性脂)、苯洗脱组分(弱极性脂)和乙醇洗脱组分(强极性脂)，并对脂溶性物质和3种洗脱组分进行抑菌活性研究，以促进地木耳的综合应用。结果表明，地木耳中脂溶性物质含量为2.62%。其中，非极性石油醚洗脱组分含量最高，占总脂的54%，但无抑菌活性；强极性的乙醇洗脱组分含量其次，占总脂的32%，其抑菌活性最强；弱极性苯洗脱组分含量最低，占总脂的14%，有弱抑菌活性。地木耳脂溶性物质对6种菌有较好的抑制作用，其抑制能力大小为：大肠杆菌>金黄色葡萄球菌>枯草芽孢杆菌>黑曲霉>酿酒酵母>假单胞杆菌。

关键词: 地木耳, 脂溶性物质, 抑菌活性

Abstract: The antibacterial effects of fat-soluble compositions from Nostoc commune Vauch. were studied in order to contribute to diverse nutritional and medicinal values of N. commune. Soxhlet extraction method was used to extract crude fats from N. commune, and antimicrobial activities of the liposoluble compounds and fractions isolated by column chromatography were assayed against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas stutzeri, Asparagillus niger and Saccharomyces cerevisiae. The results showed fat-soluble compositions were about 2.62% in N. commune. Ethanol elution fractions showed most greatly antimicrobial activities against six bacterial stains while its contents was 32% of crube fats, the benzene fraction with the yeild of 14% in crube fats had worse antimicrobial activities than ethanol elution fractions, and the petroleum ether extract was not inhibitory to six bacterial stains with the yeild of 54% in crube fats. Fat-soluble compositions had most strongly inhibitory effect on E.coli with IC₅₀ 38.75 mg/mL and MIC 64.0 mg/mL and they had least inhibitory effect on Pseudomonas stutzeri with IC₅₀ 89.58 mg/mL and MIC 106.5 mg/mL. Fat-soluble compositions showed antibacterial effects against six bacterial stains：Escherichia coli > Staphylococcus aureus > Bacillus subtilis > Asparagillus niger > Saccharomyces cerevisiae > Pseudomonas stutzeri.

Key words: Nostoc commune, Vauch. Fat-soluble compositions, Antibacterial

刁毅, 杨足君. 地木耳中脂溶性物质抑菌活性研究[J]. 生物技术通报, 2014, 0(4): 83-86.

DiaoYi, Yang Zujun,. Study on Antibacterial Effects of Fat-soluble Compositions in Nostoc commune[J]. Biotechnology Bulletin, 2014, 0(4): 83-86.

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