原始热带雨林鹦歌岭土壤抗MRSA放线菌的分离与筛选

doi:10.13560/j.cnki.biotech.bull.1985.2017-1085

摘要/Abstract

摘要： 鹦歌岭是位于海南省乐东县的原始热带雨林，从鹦歌岭土壤中分离与筛选抗耐甲氧西林金黄色葡萄球菌（MRSA）的放线菌。采用平板稀释法分离，以MRSA作为受试菌株，测试抗菌活性，对具有抗菌活性的菌株进行16S rDNA序列测定。采用滤纸片法，测定菌株发酵粗提液对MRSA抑菌活性，并用高效液相色谱仪检测粗提液的活性物质。从鹦歌岭土壤中共分离到168株放线菌，其中10株具有较强的抗MRSA活性，发酵液粗提物抑菌圈直径在8 mm-25 mm之间；16S rDNA序列比对发现，10株菌均与链霉菌属（Streptomyces）的相似性达99%以上，初步确定是链霉菌属的放线菌。通过对比10株菌发酵液HPLC指纹图谱，发现菌株7、5和9-1的发酵液次级代谢产物丰富，活性测试表明，10株放线菌对MRSA的生长均有明显的抑制作用。

关键词: 鹦歌岭, 放线菌, MRSA, 抑菌活性

Abstract: Yingge Ridge is a primitive tropical rainforest located in Ledong County，Hainan Province. In this study，the actinomycetes were isolated from the Yingge Ridge soil and screened for antibacterial activities against methicillin-resistant Staphylococcus aureus（MRSA）. Actinomycete strains were isolated by plate dilution，and MRSA was used as the target to test antibacterial activities. Strains with antibacterial activity were subjected to 16S rDNA sequence determination. The antibacterial activity of crude extract of strain was determined by filter paper method，and the active substance of crude extract was detected by high performance liquid chromatography. A total of 168 strains of actinomycetes were obtained from the soil of the Yingge Ridge. Ten of them had strong anti-MRSA activity，and the inhibition zone of the crude extract of the fermentation broth was between 8 mm and 25 mm. The identity of 16S rDNA sequences from 10 strains of anti-MRSA Actinobacteria was over 99% in comparison with those of Streptomyces in Genbank. By HPLC fingerprints of 10 strains of fermentation broth，the secondary metabolites of strain 7，5，and 9-1 were abundant，and the activity test showed that the growth of MRSA was obviously inhibited，which provided a certain scientific basis for further research.

Key words: Yingge Ridge, actinomycetes, MRSA, antibacterial activity

潘洁明 ,张荣意 ,邓加艾, 谭志琼. 原始热带雨林鹦歌岭土壤抗MRSA放线菌的分离与筛选[J]. 生物技术通报, 2018, 34(6): 128-133.

PAN Jie-ming ,ZHANG Rong-yi ,DENG Jia-ai ,TAN Zhi-qiong. Isolation and Screening of Anti-MRSA Actinomycetes in the Soil from the Primitive Tropical Rainforest Yingge Ridge[J]. Biotechnology Bulletin, 2018, 34(6): 128-133.

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