甘肃不同地区绵羊TLR9基因多态性分析

生物技术通报 ›› 2014, Vol. 0 ›› Issue (5): 107-111.

甘肃不同地区绵羊TLR9基因多态性分析

吕伟丽¹ 张小丽¹ 马小军^1，2 张国华¹

(1.甘肃农业大学动物医学院，兰州 730070；2.甘肃省草食动物生物技术重点实验室，兰州 730070)

收稿日期:2013-10-17 出版日期:2014-05-23 发布日期:2014-05-24
作者简介:吕伟丽，女，硕士研究生，研究方向：动物免疫与抗病；E-mail：350186368@qq.com
基金资助:
甘肃农业大学创新基金项目(GAU-CX1035)，甘肃省农牧厅生物技术研究与应用开发项目(GNSW-2013-24)

Polymorphisms of TLR9 Gene in Sheep in Gansu Province

Lü Weili¹ Zhang Xiaoli¹ Ma Xiaojun^1，2 Zhang Guohua¹

(1. College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070；2. Gansu Key Laboratory of Herbivorpous Animal Biotechnology，Lanzhou 730070)

Received:2013-10-17 Published:2014-05-23 Online:2014-05-24

摘要/Abstract

摘要： 针对由于急、慢性呼吸道引起的生产性能降低甚至死亡，选择与该病相关的基因TLR9为研究对象，分析群体中该基因的遗传多态性及变异特征，为进一步揭示舍饲绵羊的遗传特性和生产利用提供基础数据。采用PCR-SSCP检测427只表型正常和58只患呼吸道疾病的舍饲绵羊TLR9基因的多态性，测序群体内变异的各等位基因数列，并构建系统发育树以明确舍饲绵羊TLR9基因等位基因之间的遗传关系。结果显示，甘肃地区绵羊在TLR9基因中发现了4个等位基因，共7个核苷酸多态位点，这些位点多是由点突变形成，其中转换4个(占57.14%)，颠换3个(占42.86%)。甘肃地区绵羊TLR9基因具有较丰富的多态性；TLR9基因多态性与绵羊呼吸道疾病有一定的相关性。

关键词: 甘肃地区绵羊, TLR9基因, 多态性, PCR-SSCP

Abstract: There is a acute and chronic respiratory disease in population of sheep，which leads to productive reduces even death. Gene TLR9 is associated with respiratory disease. The Polymorphism and variation of gene TLR9 was investigated in order to find the best alleles for respiratory disease resistance in the further observation by means of phenotype and genotype，reveal the genetic characteristics of sheep and provide data for future search. Fragments of DNA containing TLR9 were amplified using PCR. Single-strand conformational polymorphism(SSCP)analysis and DNA sequence analysis were employed to detect genetic variation in 427 healthy sheep and 58 with respiratory disease. Results showed that four alleles were identified，seven nucleotide polymorphisms were in the analysis of gene sequences，which mainly came from point mutation，including transition of 4(57.14%)and transversion of 3(42.86%). Sheep TLR9 gene in Gansu has high level of sequence polymorphism. TLR9 gene polymorphisms with respiratory disease in sheep has some relevance.

Key words: Sheep in, Gansu TLR9, Gene, Genetic, Polymorphism, PCR-SSCP

吕伟丽, 张小丽, 马小军, 张国华. 甘肃不同地区绵羊TLR9基因多态性分析[J]. 生物技术通报, 2014, 0(5): 107-111.

Lü Weili, Zhang Xiaoli, Ma Xiaojun, Zhang Guohua. Polymorphisms of TLR9 Gene in Sheep in Gansu Province[J]. Biotechnology Bulletin, 2014, 0(5): 107-111.

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