金川多肋牦牛HOXC10基因的克隆、序列分析及组织表达

生物技术通报 ›› 2014, Vol. 0 ›› Issue (10): 139-143.

金川多肋牦牛HOXC10基因的克隆、序列分析及组织表达

张亚南¹, 熊显荣¹, 兰道亮¹, 符梅¹, 张雁¹, 侯定超², 马定美², 李善荣², 李键¹

1.西南民族大学生命科学与技术学院,成都 610041; 2.金川县畜牧兽医局,金川 624100

收稿日期:2014-04-22 出版日期:2014-10-20 发布日期:2014-10-17
作者简介:张亚南,女,硕士研究生,研究方向：细胞与胚胎工程
基金资助:
国家科技支撑计划（2012BAD13B06）,国家公益性行业(农业)科研专项（201203009）,西南民族大学研究生创新性项目（CX-2014SZ72）

Cloning,Sequence and Tissue Expression Pattern Analysis of HOXC10 Gene in Multi-costa Properties Yaks of Jinchuan

Zhang Yanan¹, Xiong Xianrong¹, Lan Daoliang¹, Fu Mei¹, Zhang Yan¹, Hou Dingchao², Ma Dingmei², Li Shanrong², Li Jian¹

1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041; 2. Animal Husbandry and Veterinary Bureau of Jinchuan County, Jinchuan 624100

Received:2014-04-22 Published:2014-10-20 Online:2014-10-17

摘要/Abstract

摘要： 旨在克隆牦牛HOXC10基因,并进一步分析该基因的结构与功能以及揭示其组织特异性表达规律。以金川多肋牦牛为材料,根据GenBank已公布的绵羊HOXC10基因序列设计引物,采用RT-PCR技术对HOXC10基因的cDNA全长进行扩增,并对其进行生物信息学分析及在各组织中的表达谱。对测序结果进行生物信息学分析表明,扩增的牦牛HOXC10基因长度是1 272 bp,其中包含一个1 029 bp的开放阅读框,共编码342个氨基酸;同源性分析表明,牦牛与黄牛的相似性较高,为93.2%;预测HOXC10蛋白质的分子量为38.2 kD,理论等电点为8.45;进化树分析显示,牦牛与黄牛的亲缘关系最近,处于同一个分支上;组织表达谱分析表明,该基因在牦牛各组织中均有不同程度的表达,其中在肝脏中的表达量最高,胃中的表达量最低。

关键词: 牦牛, 克隆, HOXC10, 序列分析, 组织表达

Abstract: The present study aimed at cloning yak HOXC10 gene, and further analysis of the gene structure and function, and reveals the tissue specificity expression pattern. The Jinchuan multi-costa properties yak as materials, this study based on the GenBank published Ovis aries HOXC10 gene sequences for primier designing, using RT-PCR technology to amplification the cDNA full length of HOXC10 gene, and its bioinformatic analysis and expression profiling in various tissues. The sequencing result showed that the length of yak HOXC10 gene is 1 272 bp and contains a 1 029 bp open reading frame（ORF）, encoding 342 amino acids. The results of homology analysis showed that yak and bos taurus has the higher similarity（93.2%）;Followed by predicting HOXC10 protein molecular weight 38.2 kD, the theory of isoelectric point is
8.45. Evolutionary tree analysis results showed that yak was closest relative with bos taurus, and in the same branch. Tissue expression profile analysis showed that HOXC10 gene is expressed in different degrees in various organizations, which had the highest expression in the liver, the lowest expression in the stomach.

Key words: Yak, Cloning, HOXC10, Sequence analysis, Tissue expression

张亚南, 熊显荣, 兰道亮, 符梅, 张雁, 侯定超, 马定美, 李善荣, 李键. 金川多肋牦牛HOXC10基因的克隆、序列分析及组织表达[J]. 生物技术通报, 2014, 0(10): 139-143.

Zhang Yanan, Xiong Xianrong, Lan Daoliang, Fu Mei, Zhang Yan, Hou Dingchao, Ma Dingmei, Li Shanrong, Li Jian. Cloning,Sequence and Tissue Expression Pattern Analysis of HOXC10 Gene in Multi-costa Properties Yaks of Jinchuan[J]. Biotechnology Bulletin, 2014, 0(10): 139-143.

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