生物技术通报 ›› 2014, Vol. 0 ›› Issue (11): 138-141.

• 研究报告 • 上一篇    下一篇

抗虫转基因银河杨的生长特性和抗虫性

陈虞超,张丽,巩檑,甘晓燕,石磊,宋玉霞   

  1. 宁夏农业生物技术重点实验室,银川 750002
  • 收稿日期:2014-03-07 出版日期:2014-11-07 发布日期:2014-11-07
  • 作者简介:陈虞超,男,硕士,助理研究员,研究方向:植物基因工程
  • 基金资助:
    国家林业转基因生物安全性监测项目(JJ-2012-07)

Growth Characteristic and Insect-Resistance of Transgenic Populus alba Populus hopeiensis

Chen Yuchao ,Zhang Li Gong, Lei Gan ,Xiaoyan, Shi Lei ,Song Yuxia   

  1. Key Laboratory of Agricultural Biotechnology of Ningxia, Yinchuan 750002
  • Received:2014-03-07 Published:2014-11-07 Online:2014-11-07

摘要: 以非转基因银河杨(Populus alba×Populus hopeiensis)为对照,对抗虫转基因银河杨抗虫基因Cry3A的稳定性,转基因银河杨的物候期、生长状况和抗虫性进行研究。结果发现,Cry3A基因整合稳定,在根、茎、叶中RNA和蛋白水平均有表达,不同组织中Bt蛋白水平差异显著,茎中Bt蛋白水平最高。抗虫转基因银河杨的物候期、生长状况未发生显著变化,但表现出显著的抗虫性。

关键词: 抗虫转基因银河杨, Cry3A基因, 物候期, 生长状况, 抗虫性

Abstract: The stability of insect-resistance gene Cry3A, phenophase, growth status and insect-resistance of transgenic poplar hybrid(Populus alba×Populus hopeiensis)was investigated, non- transgenic Populus alba×Populus hopeiensis as control. The results showed that the Cry3A gene in transgenic Populus alba×Populus hopeiensis integrated stably. The Cry3A gene could be expressed at RNA level and protein level in root, shoot and leaf. The Bt protein level was significantly different in different tissues, and was highest in shoot. The phenophase and growth status of transgenic Populus alba×Populus hopeiensis changed indistinctively. But it showed distinct insect-resistance.

Key words: Insect-resistance transgenic, Populus alba×, Populus hopeiensis Cry3A gene , Phenophase , Growth status Insect-resistance