海水小球藻磷酸烯醇式丙酮酸羧化酶基因（Chpepc2）克隆和生物信息学分析

摘要/Abstract

摘要： 为了进一步了解藻类PEPC酶的特征,对海水小球藻pepc2基因进行了克隆并通过生物信息学方法分析了海水小球藻PEPCase2的结构和功能特征。结果表明,海水小球藻pepc2基因与莱茵衣藻pepc2基因相似度为90%,且其对应的氨基酸序列的特征与莱茵衣藻PEPCase2特征相同,可见该基因编码的是海水小球藻细菌型PEPC酶。海水小球藻PEPCase2二级结构主要包括α螺旋、β转角、无规则卷曲和伸展链,其三级结构外部为α螺旋结构,中心为平行β桶结构。海水小球藻PEPCase2具有多种重要的生理功能,主要与多种重要的物质合成有关。

关键词: 海水小球藻, PEPC酶, 生物信息学, 结构, 功能

Abstract: To further understand the characteristics of algae PEPCase, marine Chlorella sp. pepc2 gene was cloned and analyzed the structural and functional characteristics of marine Chlorella sp. PEPCase2 by bioinformatics method. The experimental results showed that, the cDNA sequence of marine Chlorella sp. pepc2 gene, compared with the cDNA sequence of Chlamydomonas reinhardtii pepc2 gene, the similarity was 90%, and its amino acid sequence has the same characteristics with C. reinhardtii PEPCase2, so this gene encodes the bacterial-type PEPCase of marine Chlorella sp.；The secondary structures of marine Chlorella sp. PEPCase2 mainly include α-helix, β-turn, random coli and extended strand, and its tertiary structure is a parallel β-barrel structure in the centre with α-helix structures outside；marine Chlorella sp. PEPCase2 has many important physiological functions, mainly related to a variety of important material synthesis.

Key words: Marine Chlorella sp., Phosphoenolpyruvate carboxylase, Bioinformatics , Structure , Function

黄希文,施定基,贾晓会,田琪琳,贾睿,何培民. 海水小球藻磷酸烯醇式丙酮酸羧化酶基因（Chpepc2）克隆和生物信息学分析[J]. 生物技术通报, 2014, 0(11): 164-173.

Huang Xiwen,Shi Dingji,Jia Xiaohui,Tian Qilin,Jia Rui,He Peimin. Cloning and Bioinformatics Analysis of the Phosphoenolpyruvate Carboxylase(Chpepc2)Gene from Marine Chlorella sp.[J]. Biotechnology Bulletin, 2014, 0(11): 164-173.

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