红花油体提取条件优化及稳定性研究

doi:10.13560/j.cnki.biotech.bull.1985.2015.04.027

生物技术通报 ›› 2015, Vol. 31 ›› Issue (3): 185-190.doi: 10.13560/j.cnki.biotech.bull.1985.2015.04.027

红花油体提取条件优化及稳定性研究

杨晶^1，2, 韩高强², 刘忠良², 卢振², 吴治邦², 王清曼², 邹德毅², 强卫东², 陈伟²

(1.吉林农业大学生物反应器与药物开发教育部工程研究中心，长春 130118；2.吉林农业大学生命科学学院，长春 130118)

收稿日期:2014-08-04 出版日期:2015-03-16 发布日期:2015-03-16
作者简介:杨晶，女，博士，副研究员，研究方向：植物生物反应器；E-mail：yangjing5122010@163.com
基金资助:
十二五国家高新技术研究与发展计划项目(“863”计划项目)，2012年国家大学生创新创业项目(2011AA100606)，国家教育部博士点基金-青年教师基金项目(20122223120002)，吉林省中医药管理局项目(2012162)，吉林省教育厅项目(201458)

Optimization of Extract Condition of Safflower Oil Body and Analysis of Stability

Yang Jing^1，2 Han Gaoqiang² Liu Zhongliang² Lu Zhen² Wu Zhibang² Wang Qingman² Zou Deyi²Qiang Weidong² Chen Wei²

(1. Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development，Changchun 130118；2. College of Life Sciences，Jilin Agricultural University，Changchun 130118)

Received:2014-08-04 Published:2015-03-16 Online:2015-03-16

摘要/Abstract

摘要： 油体是储藏脂肪的亚细胞单位，其表面包裹一层磷脂和油体蛋白。这种稳定的结构可以保护油体面对环境的压力，使油体可以应用在食品、化妆品及制药工业中。研究红花油体的提取方法及红花油体乳液的基本性质，旨在为以油体为基质的载体体系研究奠定基础。以pBS为介质，采用梯度离心法，比较了不同提取条件对红花油体的提取效率的影响；对其在不同pH值、NaCl浓度条件下红花油体的平均粒径和稳定性进行测定。结果表明，红花油体在pH值≥6条件下，平均粒径为1.75-2.05 μm和pH值≤6条件下，平均粒径1.50-1.75 μm；NaCl浓度 0.2和0.4 mg/mL时，红花油体分散较为均匀，NaCl浓度 1.2和2.0 mg/mL时，红花油体出现聚集现象。蔗糖浓度0.1和0.2 mg/mL时，红花油体分散较为均匀，蔗糖浓度为0.4-1.0 mg/mL时，红花油体比较密集，随着蔗糖浓度的增加，红花油体的粒径逐渐开始不均一。红花油体的最佳提取条件是pH7，NaCl浓度0.2 mg/mL，蔗糖浓度0.1 mg/mL，稀释后的红花油体溶液在不加入保护剂或者不经过物理方法处理下，保存起来不稳定。

关键词: 红花, 油体, 提取, 粒径, 稳定性

Abstract: In order to achieve a wide range of applications in many fields, especially on oil body as a substrate carrier system of the foundation, extraction method of safflower oil and characteristics of safflower oil body lotion was researched. Extraction efficiency of safflower oil body was compared, including its value at different pH, NaCl concentration conditions, an average particle size of safflower oil and stability, as well as its viscosity to conduct investigations. Results showed that the safflower oil body dispersed more evenly, at pH≥6, the average particle size of 1.75-2.05 μm and pH ≤ 6 conditions, the average particle size of 1.50-1.75 μm; while NaCl concentration of 0.2 and 0.4 mg/mL, NaCl concentration 1.2 and 2.0 mg/mL, the safflower oil bodies appeared aggregation. When sucrose concentration between 0.1 and 0.2 mg/mL, safflower oil dispersion is uniform; when the sugar concentration of 0.4-1.0 mg/mL, safflower body intensive, with increasing concentration of sucrose, safflower oil body size gradually began to heterogeneity. The optimum extraction condition for safflower oil body is pH7, NaCl 0.2 mg/mL, sucrose concentration of 0.1 mg/mL. The study proved that safflower oil body without adding detergent or without physical approach, the saved is unstable.

Key words: safflower, oil-body, extraction, size, stability

杨晶, 韩高强, 刘忠良, 卢振, 吴治邦, 王清曼, 邹德毅, 强卫东, 陈伟. 红花油体提取条件优化及稳定性研究[J]. 生物技术通报, 2015, 31(3): 185-190.

Yang Jing, Han Gaoqiang, Liu Zhongliang, Lu Zhen, Wu Zhibang, Wang Qingman, Zou Deyi,Qiang Weidong, Chen Wei. Optimization of Extract Condition of Safflower Oil Body and Analysis of Stability[J]. Biotechnology Bulletin, 2015, 31(3): 185-190.

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红花油体提取条件优化及稳定性研究

Optimization of Extract Condition of Safflower Oil Body and Analysis of Stability

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