17β-雌二醇对斑马鱼性别分化的影响

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.032

摘要/Abstract

摘要： 有报道表明，经17β-雌二醇(17β-estradiol，E2)处理后，斑马鱼种群中雌性比例上升。为了研究E2雌性化作用的机制，选取一系列斑马鱼体内与性别分化有关的基因(brca2, sox9a, sox9b, dmrt1和 cyp19a1a)，采用qRT-PCR分析它们在不同的处理条件下表达水平的变化。结果表明，E2能上调雌性相关基因(brca2、sox9b)的表达，下调部分雄性相关基因(sox9a)的表达，对不同发育阶段的斑马鱼体内的性激素转化通路(cyp19a1a)的影响不同。

关键词: 环境内分泌干扰物, 17β-雌二醇, 斑马鱼, 性别分化

Abstract: Previous studies have demonstrated that 17β-estradiol(E2)could increase the female proportion in zebrafish population. In order to study mechanisms of feminization caused by E2, quantitative real-time PCR(qRT-PCR)was used to analyze expression levels of genes involved in sexual differentiation(brca2, sox9a, sox9b, dmrt1 and cyp19a1a)under different conditions. The results indicate that E2 can up-regulate the expression levels of the female-predominant genes(brac2 and sox9b), partially down-regulate the expression levels of male-predominant gene(sox9a)and has different effects on sex hormone conversion pathway(cyp19a1a)in different developmental stages of zebrafish.

Key words: endocrine disrupting chemicals, 17β-estradiol, zebrafish, sexual differentiation

李国超, 余凯敏, 冯为民, 刘丽丽, 张家禹, 闫艳春. 17β-雌二醇对斑马鱼性别分化的影响[J]. 生物技术通报, 2015, 31(6): 200-208.

Li Guochao, Yu Kaimin, Feng Weimin, Liu Lili, Zhang Jiayu, Yan Yanchun. Effects of 17β-estradiol on the Sex Differentiation of Zebrafish(Danio rerio)[J]. Biotechnology Bulletin, 2015, 31(6): 200-208.

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