锑在斑马鱼不同组织中的积累及其对抗氧化系统的影响

doi:10.13560/j.cnki.biotech.bull.1985.2020-1060

摘要/Abstract

摘要：

研究在不同Sb浓度胁迫下,斑马鱼各组织中Sb的积累以及各类酶活性及含量变化。以斑马鱼为受试对象,暴露于Sb溶液（0、13.26、26.53、39.79 mg/L）中14 d后,测定各组织Sb积累量、SOD、CAT活性及GSH、MDA含量。结果显示Sb在各组织中积累顺序为肝脏>鳃>肌肉>脑,且随着Sb胁迫浓度增大而逐渐增多。随着Sb胁迫浓度增高,肝脏、脑、鳃、肌肉SOD活性呈现不同变化趋势;CAT活性及GSH含量均呈先上升后下降的趋势,表现为低浓度促进,高浓度抑制;MDA含量呈逐渐升高的趋势。综上表明,Sb可在斑马鱼不同组织积累,激活机体抗氧化防御系统,并使机体产生脂质过氧化。

关键词: 锑, 斑马鱼, 积累, 抗氧化, 脂质过氧化

Abstract:

This study aimed to study the accumulation of antimony in zebrafish tissues and changes in various enzyme activities and contents under the stress of different concentration of antimony. Danio rerio were exposed to antimony solution（0,13.26,26.53,and 39.79 mg/L）for 14 d to determine the accumulation of antimony,the activity of superoxide dismutase（SOD）,catalase（CAT）,content of glutathione（GSH）and malondialdehyde（MDA）in each tissue. The results showed that the order of antimony accumulation in each tissue was as：Liver > Gill> Muscle > Brain and it gradually increased with the increase of antimony stress concentration. With the increased of antimony stress concentration,SOD activity in the liver,brain,gills and muscles presented different trends;CAT activity and GSH content both increased first and then decreased,which was as promotion at low concentration and inhibition at high concentration;MDA content tended gradually increasing. In conclusion,antimony can accumulate in different tissues of Danio rerio,activating antioxidant defense system and causing lipid peroxidation.

Key words: antimony, zebrafish, accumulation, antioxidant, lipid peroxidation

徐琨, 杨爱江, 胡霞, 邹海洮, 李彬, 刘吉. 锑在斑马鱼不同组织中的积累及其对抗氧化系统的影响[J]. 生物技术通报, 2021, 37(4): 145-154.

XU Kun, YANG Ai-jiang, HU Xia, ZOU Hai-tao, LI Bin, LIU Ji. Antimony Accumulation and Its Effect on Antioxidation System in Different Tissues of Danio rerio[J]. Biotechnology Bulletin, 2021, 37(4): 145-154.

图/表 5

图1 对照组和不同浓度锑处理组中斑马鱼肝、脑、鳃、肌肉中总锑含量对照组浓度ND（未检测出）,不同字母表示各组间差异显著（P<0.05）,（n=3）下同

Fig.1 Total antimony content in liver, brain, gill and muscle of zebrafish under different concentrations of antimony treatment The concentration in the control group was ND (not detected), and different letters indicate significant differences between the groups (P<0.05),the same below(n=3)

图2 不同浓度锑处理组中斑马鱼肝、脑、鳃、肌肉SOD活性

Fig.2 SOD activity in liver, brain, gill and muscle of zebrafish under different concentrations of antimony treatment

图3 不同浓度锑处理组中斑马鱼肝、脑、鳃、肌肉CAT活性

Fig.3 SOD activity in liver, brain, gill and muscle of zebrafish under different concentrations of antimony treatment

图4 不同浓度锑处理组中斑马鱼肝、脑、鳃、肌肉GSH含量

Fig.4 GSH content in liver, brain, gill and muscle of zebrafish under different concentrations of antimony treatment

图5 不同浓度锑处理组中斑马鱼肝、脑、鳃、肌肉MDA含量

Fig.5 MDA content in liver, brain, gill and muscle of zebrafish under different concentrations of antimony treatment

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