基于高通量测序技术检测全基因组DNA甲基化水平的方法

doi:10.13560/j.cnki.biotech.bull.1985.2015.07.007

摘要/Abstract

摘要：

DNA甲基化的研究最近几年一直是表观遗传学研究的重点。有关DNA甲基化水平检测的方法大体上有十几种，随着第二代测序技术的发展，实现了在全基因组水平上对甲基化状态进行检测。目前，基于第二代高通量测序进行全基因组DNA甲基化水平检测的方法主要有BSP-seq(亚硫酸氢盐修饰结合直接测序法)、MeDIP-seq(甲基化DNA免疫共沉淀测序法)、MBD-seq(甲基化DNA富集结合高通量测序法)。就这3种方法在原理、流程、优缺点、优化使用及后期需要用到的部分生物信息学资源等方面的研究进展作一综述，旨在为研究者在采用高通量测序方法研究DNA甲基化模式时提供一些思路。

关键词: 高通量测序技术, 全基因组, DNA甲基化水平, BSP-seq, MeDIP-seq, MBD-seq

Abstract:

DNA methylation has been being the research focus of epigenetics study in the recent years. There are over 10 kinds of methods for detecting DNA methylation level, and detection of methylation status on genome-wide level has been achieved with the development of the NGS(Next-Generation Sequencing). At present the major detection methods of DNA methylation level on genome-wide scope based on NGS include BSP-seq(Bisulfite Sequencing PCR), MeDIP-seq(Methylated DNA Immunoprecipitation Sequencing)and MBD-seq(Methylated DNA Binding Domain Sequencing). In this paper, the research progress of principles, procedures, advantages and disadvantages, optimization of the usage and some bioinformatics resources on these 3 methods were reviewed, which aims to provide some beneficial ideas to researchers who are studying DNA methylation models while employing methods of high-throughput sequencing.

Key words: high-throughput sequencing technology, genome-wide, DNA methylation level, BSP-seq, MeDIP-seq, MBD-seq

马浪浪, 梁振娟, 先新, 罗仕文, 李清超, 梁黔云. 基于高通量测序技术检测全基因组DNA甲基化水平的方法[J]. 生物技术通报, 2015, 31(7): 45-50.

Ma Langlang, Liang Zhenjuan, Xian Xin, Luo Shiwen, Li Qingchao, Liang Qianyun. Detection Methods of Genome-wide DNA Methylation Level Based on High-throughput Sequencing Technology[J]. Biotechnology Bulletin, 2015, 31(7): 45-50.

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