诱导烟草悬浮细胞氧爆发的海绵共附生真菌HMP-F66的筛选与鉴定

doi:10.13560/j.cnki.biotech.bull.1985.2015.07.026

生物技术通报 ›› 2015, Vol. 31 ›› Issue (7): 174-179.doi: 10.13560/j.cnki.biotech.bull.1985.2015.07.026

诱导烟草悬浮细胞氧爆发的海绵共附生真菌HMP-F66的筛选与鉴定

庞志伟^1，2, 路旭^2，3, 胡江春² , 陈晓琦^2，3, 王楠², 宋艳玲¹

(1.沈阳化工大学制药与生物工程学院，沈阳 110142；2.中国科学院沈阳应用生态研究所，沈阳 110016；中国科学院大学，北京 100049)

收稿日期:2014-12-01 出版日期:2015-07-16 发布日期:2015-07-16
作者简介:庞志伟，男，硕士研究生，研究方向：海洋微生物活性代谢物；E-mail：503492138@qq.com
基金资助:
国家自然科学基金项目(31471814)

Screening and Identification of a Sponge-associated Fungus HMP-F66 Inducing Oxidative Burst in Tobacco Cell Suspensions

Pang Zhiwei^1，2, Lu Xu^2，3, Hu Jiangchun², Cheng Xiaoqi^2，3, Wang Nan², Song Yanling¹

(1. College of Pharmaceutical and Biological Engineering，Shenyang University of Chemical Technology，Shenyang 110142；2. Institute of Applied Ecology，Chinese Academy of Sciences，Shenyang 110016；3. University of Chinese Academy of Sciences，Beijing 100049)

Received:2014-12-01 Published:2015-07-16 Online:2015-07-16

摘要/Abstract

摘要： 激发植物本身的天然免疫系统可作为防控植物病害的新途径。以50株分离自繁茂膜海绵的真菌为受试菌株筛选具有诱导植物抗性活性的菌株及其代谢产物。采用基于H₂DCF-DA的荧光酶标法考察这些真菌的粗代谢物诱导烟草悬浮细胞产生氧爆发的活性，最终筛选到一株真菌HMP-F66能够显著诱导烟草悬浮细胞产生活性氧。根据其形态学特征、培养特征、特征性生理生化反应以及基于rDNA ITS的分子生物学分析，推测该菌株为米曲霉(Aspergillus oryzae)。

关键词: 米曲霉, 黄曲霉, 繁茂膜海绵, 氧爆发, 诱导抗性

Abstract: The plant innate immune system provides an alternative way for crop protection, and the plant immunity triggers have been an important source of biorational pesticide leads. In order to find new plant resistance inducers, fifty sponge-associated fungal isolates were screened for their ability to induce oxidative burst in the tobacco suspensions using the H2DCF-DA fluorescence assay. Strain HMP-F66 showed significant activity in inducing H2O2 production in tobacco suspensions. HMP-F66 was assumed to be Aspergillus oryzae by its morphological, physiological, cultural characters, and rDNA ITS sequence analysis.

Key words: Aspergillus oryzae, Aspergillus flavus, Hymeniacidon perleve, oxidative burst, induced resistance

庞志伟, 路旭, 胡江春 , 陈晓琦, 王楠, 宋艳玲. 诱导烟草悬浮细胞氧爆发的海绵共附生真菌HMP-F66的筛选与鉴定[J]. 生物技术通报, 2015, 31(7): 174-179.

Pang Zhiwei, Lu Xu, Hu Jiangchun, Cheng Xiaoqi, Wang Nan, Song Yanling. Screening and Identification of a Sponge-associated Fungus HMP-F66 Inducing Oxidative Burst in Tobacco Cell Suspensions[J]. Biotechnology Bulletin, 2015, 31(7): 174-179.

参考文献

[1]Samson R. Current Systematics of the Genus Aspergillus[M]//. Powell, KA, Renwick A, Peberdy JF. The Genus Aspergillus from taxonomy and genetics to industrial application. New York：Plenum Press, 1994：261-276.
[2]Bennett JW. An overview of the genus Aspergillus[M]//. Machida M, Gomi K. Aspergillus：Molecular Biology and Genomics. Norfolk：Caiser Academic Press, 2010：1-17.
[3]Pfefferle W, Anke H, Bross M, et al. Asperfuran, a novel antifungal metabolite from Aspergillus oryzae[J]. The Journal of antibiotics, 1990, 43(6)：648-654.
[4]Hu X, Xia QW, Zhao YY, et al. Speradines F-H, Three new oxindole alkaloids from the marine-derived fungus Aspergillus oryzae[J]. Chemical and Pharmaceutical Bulletin, 2014, 62(9)：942-946.
[5]Camejo D, Martí MC, Jiménez A, et al. Effect of oligogalacturonides on root length, extracellular alkalinization and O^2--accumulation in alfalfa[J]. Journal of Plant Physiology, 2011, 168(6)：566-575.
[6]Felix G, Regenass M, Boller T. Specific perception of subnanomolar concentrations of chitin fragments by tomato cells：induction of extracellular alkalinization, changes in protein phosphorylation, and establishment of a refractory state[J]. The Plant Journal, 1993, 4(2)：307-316.
[7]O'Brien JA, Daudi A, Butt VS, et al. Reactive oxygen species and their role in plant defence and cell wall metabolism[J]. Planta, 2012, 236(3)：765-779.
[8]Apel K, Hirt H. REACTIVE OXYGEN SPECIES：metabolism, oxidative stress, and signal transduction[J]. Annual Review of Plant Biology, 2004, 55(1)：373-399.
[9]Grant M, Lamb C. Systemic immunity[J]. Current Opinion in Plant Biology, 2006, 9(4)：414-420.
[10] Hammond-Kosack K, Jones JD. Plant Environment and Agriculture：Responses to plant pathogens[M]//. Buchanan B, Gruissem W, Jones RL. Biochemistry and Molecular Biology of Plants. Jonh Wiley & Sons, 2000：1102-1156.
[11] Fobert PR, Despres C. Redox control of systemic acquired resistance[J]. Curr Opin Plant Biol, 2005, 8(4)：378-382.
[12] Chen X, Mou Y, Ling J, et al. Cyclic dipeptides produced by fungus Eupenicillium brefeldianum HMP-F96 induced extracellular alkalinization and H₂O₂ production in tobacco cell suspensions[J]. World Journal of Microbiology and Biotechnology, 31(1)：247-253.
[13]Gerber I， Dubery I. Fluorescence microplate assay for the detection of oxidative burst products in tobacco cell suspensions using 2', 7'-dichlorofluorescein[J]. Methods in Cell Science, 2004, 25(3-4)：115-122.
[14]齐祖同, 孔华忠, 孙曾美. 曲霉属及其相关有性型[M]//. 齐祖同. 中国真菌志. 北京：科学出版社, 1997：76-91.
[15]Murakami H. Classification of the Koji Mold[J]. The Journal of General and Applied Microbiology, 1971, 17(4)：281-309.
[16]Christensen M. A synoptic key and evaluation of species in the Aspergillus flavus group[J]. Mycologia, 1981, 73(6)：1056-1084.
[17]Kurtzman CP, Smiley MJ, Robnett CJ, et al. DNA Relatedness among wild and domesticated species in the Aspergillus flavus group[J]. Mycologia, 1986, 78(6)：955-959.
[18]Machida M, Asai K, Sano M, et al. Genome sequencing and analysis of Aspergillus oryzae[J]. Nature, 2005, 438(7071)：1157-1161.
[19]Amaike S, Keller NP. Aspergillus flavus[J]. Annual Review of Phytopathology, 2011, 49(1)：107-133.
[20]Geiser DM, Pitt JI, Taylor JW. Cryptic speciation and recombination in the aflatoxin-producing fungus Aspergillus flavus[J]. Proceedings of the National Academy of Sciences, 1998, 95(1)：388-393.
[21]Chang PK, Ehrlich KC. What does genetic diversity of Aspergillus flavus tell us about Aspergillus oryzae?[J]. International Journal of Food Microbiology, 2010, 138(3)：189-199.
[22] J?rgensen TR. Identification and toxigenic potential of the industr-ially important fungi, Aspergillus oryzae and Aspergillus sojae[J]. Journal of Food Protection, 2007, 70(12)：2916-2934.
[23]王楠, 刘祎, 胡江春, 等. 一种环匹阿尼酸类化合物及其制备和应用：中国, 201110447701. 6[P]. 2013-07-03.
[24]Bari R, Jones JG. Role of plant hormones in plant defence respon-ses[J]. Plant Molecular Biology, 2009, 69(4)：473-488.
[25]Chen Z, Agnew JL, Cohen JD, et al. Pseudomonas syringae type III effector AvrRpt2 alters Arabidopsis thaliana auxin physiology[J]. Proceedings of the National Academy of Sciences, 2007, 104(50)：20131-20136.
[26]Navarro L, Dunoyer P, Jay F, et al. A plant miRNA contributes to antibacterial resistance by repressing auxin signaling[J]. Science, 2006, 312(5772)：436-439.

诱导烟草悬浮细胞氧爆发的海绵共附生真菌HMP-F66的筛选与鉴定

Screening and Identification of a Sponge-associated Fungus HMP-F66 Inducing Oxidative Burst in Tobacco Cell Suspensions

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	杨冬, 唐璎. 枯草芽孢杆菌WTX1胞外酶降解AFB₁酶学特性及降解位点分析[J]. 生物技术通报, 2023, 39(4): 93-102.
[2]	史亚楠, 王德培, 王一川, 周昊, 薛鲜丽. 敲除msn2对米曲霉生长和发酵产曲酸的影响[J]. 生物技术通报, 2022, 38(8): 188-197.
[3]	赵雅茹, 许庆方, 高文俊, 郭刚, 陈雷, 玉柱. 抑霉乳酸菌脱毒特性及青贮应用的研究[J]. 生物技术通报, 2021, 37(9): 95-105.
[4]	唐璎, 黄佳, 邓展瑞, 杨晓楠. 一株枯草芽孢杆菌降解黄曲霉毒素B₁产物分析[J]. 生物技术通报, 2021, 37(12): 82-90.
[5]	王卫雄, 沈博, 贾洪柏, 乔俊卿, 牛犇. 根际生防菌群的应用及其防病增效的潜在机制[J]. 生物技术通报, 2020, 36(9): 31-41.
[6]	刘端木, 吴怿, 刘沄, 梁志宏. 一株产毒曲霉拮抗细菌的筛选、鉴定及抑菌活性研究[J]. 生物技术通报, 2019, 35(8): 42-50.
[7]	吴琴琴, 孙敏, 陈雨, 付雅琴, 曾斌, 贺斌. 米曲霉功能基因组研究策略和进展[J]. 生物技术通报, 2019, 35(8): 186-192.
[8]	张智敏, 庄淼, 金锋杰. 米曲霉基因工程技术的进展[J]. 生物技术通报, 2018, 34(9): 170-176.
[9]	耿龙泼, 王鑫旺, 黄路华, 邓基利, 汪世华, 张峰. 黄曲霉核糖体蛋白基因在不同生长时期的表达分析[J]. 生物技术通报, 2018, 34(4): 194-200.
[10]	任亚林, 李耘, 吴静. AFB1和ZEN对HepG2细胞体外联合毒性效应及机制研究[J]. 生物技术通报, 2018, 34(11): 160-167.
[11]	彭智超, 仝赞华, 邱德文. 蛋白激发子PeaT1与井冈霉素混合使用诱导烟草对TMV抗性的研究[J]. 生物技术通报, 2017, 33(11): 92-95.
[12]	曾昆,杜道林,薛永来. 基于特异性生物识别分子的黄曲霉毒素快速分析方法研究进展[J]. 生物技术通报, 2016, 32(8): 47-55.
[13]	汤鸣强;薛盛果;叶鹏;. 米曲霉F-81产中性蛋白酶的固定化条件及其特性[J]. , 2012, 0(03): 159-165.
[14]	路子显;伍松陵;孙长坡;. 黄曲霉素合成相关基因表达与环境因素的关系[J]. , 2010, 0(11): 56-61.
[15]	汤鸣强;曾小芳;. 米曲霉F-81菌株产中性蛋白酶的分离纯化[J]. , 2010, 0(04): 194-197.