miR319a及其靶基因在木薯中的低温响应分析

doi:10.13560/j.cnki.biotech.bull.1985.2015.11.015

生物技术通报 ›› 2015, Vol. 31 ›› Issue (11): 173-178.doi: 10.13560/j.cnki.biotech.bull.1985.2015.11.015

miR319a及其靶基因在木薯中的低温响应分析

曾长英, 周玉飞, 彭明

中国热带农业科学院热带生物技术研究所，海口 571101

收稿日期:2015-05-15 出版日期:2015-11-26 发布日期:2015-11-26
作者简介:曾长英，女，博士，研究方向: 木薯抗逆机理；E-mail: zengchangying@itbb.org.cn
基金资助:
国家“863”计划项目(2012AA101204-2)，海南省自然科学基金项目(313075)，海南省重大科技项目子课题(ZDZX2013023-1)，中央级公益性科研院所基本科研业务费(ITBB2015RC01)

The Differential Expression Characteristics of miR319 and Four Targets Response to Low Temperature Treatments in Two Cassava Varieties

Zeng Changying, Zhou Yufei, Peng Ming

Institute of Tropical Bioscience and Biotechnology，Chinese Academy of Tropical Agricultural Sciences，Haikou 571101

Received:2015-05-15 Published:2015-11-26 Online:2015-11-26

摘要/Abstract

摘要： 旨在探索miRNA在木薯低温逆境中的调控作用，前期小RNA测序筛选出miR319对低温显著响应，通过实时定量PCR方法分析miR319与其4个不同靶基因在两个木薯品种的4种低温处理下的差异表达情况。结果显示，miRNA与其靶基因在两个不同木薯品种间的响应不同，4个低温处理间的响应也不同，miRNA与各个靶基因的相关系数也不同。比较发现在C4中miR319a与3个靶基因(GSTU8除外)负相关性均好于SC124，处理之间在NAH中的负相关表现最典型，miR319与4个靶基因之间的相关系数从大到小的顺序为: MYB33>Unknown>TCP4>GSTU8，预示着MYB33和Unknown(021030m)两个靶基因在C4的NAH中是miR319a更为优先的调控目标。结果表明在木薯的低温逆境适应过程中miR319对MYB33和Unknown两个靶基因的调控作用值得深入研究。

关键词: miR319, 耐低温, 靶基因, 负向调控, 相关系数

Abstract: To study the regulation roles of microRNAs in cassava low temperature adaption, our early small RNA transcriptome profile identified miR319 response to low temperature in cassava. This study, we assayed the expression patterns of miR319 and its 4 different target genes between two cassava varieties under 4 low temperature treatments based on real-time quantitative polymerase chain reaction(PCR)technique. The results showed that the expression patterns of miR319a and its target genes differed in two different cassava varieties, differed among the four cryogenic treatments, and same to the correlation coefficients between microRNAs and four different target genes. After the comparison study, we found that the negative correlations between miR319 with three targets(MYB33, TCP4 and Unknown)are larger in C4 than those in SC124, and the NAH treatment has most typical negative correlation, and the order of negative correlation coefficients between miR319 and its target gene from large to small are: MYB33>Unknown>TCP4>GSTU8. All those data reveal that MYB33 and Unknown target(021030m)are priority target genes miR319 might execute its negative regulation under NAH treatment in C4, and thus the regulation of miR319 on MYB33 and unknown target in cassava low temperature adoption is worth in-depth study in the future.

Key words: miR319, chilling tolerance, targets, negative regulation, correlation coefficients

曾长英, 周玉飞, 彭明. miR319a及其靶基因在木薯中的低温响应分析[J]. 生物技术通报, 2015, 31(11): 173-178.

Zeng Changying, Zhou Yufei, Peng Ming. The Differential Expression Characteristics of miR319 and Four Targets Response to Low Temperature Treatments in Two Cassava Varieties[J]. Biotechnology Bulletin, 2015, 31(11): 173-178.

参考文献

[1]Jones-Rhoades MW， Bartel DP. Computational identification of plant microRNAs and their targets, including a stress-induced miRNA[J]. Molecular Cell, 2004, 14(6): 787-799.
[2] Schwab R, Palatnik JF, Riester M, et al. Specific effects of microRNAs on the plant transcriptome[J]. Development Cell, 2005, 8(4): 517-527.
[3]Palatnik JF, Allen E, Wu XL, et al. Control of leaf morphogenesis by microRNAs[J]. Nature, 2003, 425(6955): 257-263.
[4]Bartlett ME, Specht CD. Changes in expression pattern of the teosinte branched l-like genes in the Zingiberales provide a mechanism for evolutionary shifts in symmetry across the order[J]. American Journal of Botany, 2011, 98(2): 227-243.
[5]Schommer C, Palatnik JF, Aggarwal P, et al. Control of jasmonate biosynthesis and senescence by miR319 targets[J]. PLoS Biology, 2008, 6(9): e230.
[6]Nag A, King S, Jack T. miR319a targeting of TCP4 is critical for petal growth and development in Arabidopsis[J]. Proc Natl Acad Sci USA, 2009, 106(52): 22534-22539.
[7]Sunkar R, Zhu JK. Novel and stress-regulated microRNAs and other small RNAs from Arabidopsis[J]. Plant Cell, 2004, 16(8): 2001-2019.
[8]贾蓓. 水稻miR319的耐冷功能分析与分子机制研究[D]. 哈尔滨: 东北农业大学, 2012.
[9]WangST, Sun XL, Hoshino Y, et al. MicroRNA319 positively regulates cold tolerance by targeting OsPCF6andOsTCP21in rice(Oryza sativaL. )[J]. PLoS One, 2014, 9(3): e91357.
[10]Wang X. A PCR-based platform for microRNA expression profiling studies[J]. RNA, 2009, 15(4): 716-723.
[11]周玉飞, 曾长英, 陈新, 等. 低温驯化对木薯耐寒性形态、生理特性的影响[J]. 热带农业科学, 2011, 31(6): 1-6.
[12] 罗茂, 张志明, 高健, 等. miR319在植物器官发育中的调控作用[J]. 遗传, 2011, 33(11): 1203-1211.
[13]Zeng CY, Chen Z, Xia J, et al. Chilling acclimation provides immunity to stress by altering regulatory networks and inducing genes with protective functions in cassava[J]. BMC Plant Biology, 2014, 14: 207.
[14]Millar AA, Gubler F. The Arabidopsis GAMYB-like genes, MYB33 and MYB65, are microRNA-regulated genes that redundantly facilitate anther development[J]. Plant Cell, 2005, 17(3): 705-721.

[1]	史建磊, 宰文珊, 苏世闻, 付存念, 熊自立. 番茄青枯病抗性相关miRNA的鉴定与表达分析[J]. 生物技术通报, 2023, 39(5): 233-242.
[2]	王楠楠, 王文佳, 朱强. 植物胁迫相关microRNA研究进展[J]. 生物技术通报, 2022, 38(8): 1-11.
[3]	寇航, 王艳梅, 李彤, 薄明井, 张惟材, 熊向华, 黎明. 基于Methylovorus sp. J1-1基因组尺度代谢网络优化吡咯喹啉醌合成[J]. 生物技术通报, 2022, 38(2): 173-183.
[4]	张廷焕, 郭宗义, 柴捷, 潘红梅, 张亮, 陈磊, 龙熙. 序列变异对miR-378生物发生以及靶标关系的影响[J]. 生物技术通报, 2022, 38(1): 205-214.
[5]	洪军, 卫夏怡, 吉冰洁, 叶延欣, 程天赐. 铜绿假单胞菌对鲎素耐药前后的差异表达基因及SNP变化研究[J]. 生物技术通报, 2021, 37(9): 191-202.
[6]	李玲, 杨丽霞, 郭梅. CNR转录因子在番茄果实成熟过程中的功能[J]. 生物技术通报, 2021, 37(2): 51-62.
[7]	张廷焕, 龙熙, 郭宗义, 柴捷. miR-378促进脂质生成相关靶基因鉴定[J]. 生物技术通报, 2021, 37(2): 80-87.
[8]	李泽卿, 刘彩贤, 邢文, 文亚峰. miRNA在植物响应高温胁迫中的研究进展[J]. 生物技术通报, 2020, 36(2): 149-157.
[9]	胡积祥, 曹雅倩, 朱秀梅, 余超, 田芳, 杨凤环, 陈华民, 何晨阳. 基于瞬时表达系统的水稻miRNA靶基因快速验证系统的建立[J]. 生物技术通报, 2019, 35(10): 57-63.
[10]	谢洁 ,王明 ,李青 ,潘妃 ,熊兴耀 ,秦玉芝. 植物miR390的研究进展[J]. 生物技术通报, 2018, 34(6): 1-10.
[11]	刘伟灿,周永刚,王兴超,王法微,王南,董园园,李晓薇,李海燕. 植物MicroRNA介导的基因调控在作物改良中的应用潜能[J]. 生物技术通报, 2016, 32(4): 6-15.
[12]	王维,张玉娟,陈洁,刘聚波,夏民旋,沈法富. 植物逆境胁迫相关miRNA研究进展[J]. 生物技术通报, 2015, 31(1): 1-10.
[13]	李静,黄英,黄丽梅,杨明华,李琦华,贾俊静,赵素梅. miR-143在脂肪细胞分化和脂类代谢中的作用[J]. 生物技术通报, 2014, 0(9): 34-38.
[14]	李婧;熊莉丽;胡久梅;郭志云;. 基于EST和GSS序列的玉米未知微RNA的数据挖掘[J]. , 2011, 0(12): 108-112.
[15]	彭娟;李思光;罗丹丹;罗玉萍;. miR-124基因家族的分子进化与靶基因预测[J]. , 2011, 0(10): 167-178.

miR319a及其靶基因在木薯中的低温响应分析

The Differential Expression Characteristics of miR319 and Four Targets Response to Low Temperature Treatments in Two Cassava Varieties

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价