星星草PtLIR1基因克隆及其在NaCl胁迫下的表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2015.11.022

生物技术通报 ›› 2015, Vol. 31 ›› Issue (11): 166-172.doi: 10.13560/j.cnki.biotech.bull.1985.2015.11.022

星星草PtLIR1基因克隆及其在NaCl胁迫下的表达分析

周琪^1,2, 罗春², 郭敏², 付畅^1,2

1. 植物生物学黑龙江省高校重点实验室哈尔滨师范大学，哈尔滨 150025；
2. 哈尔滨师范大学生命科学与技术学院，哈尔滨150025

收稿日期:2015-05-06 出版日期:2015-11-26 发布日期:2015-11-26
作者简介:周琪，女，硕士研究生，研究方向: 植物抗逆分子生物学；E-mail: 1185713326@qq.com
基金资助:
植物生物学黑龙江省高校重点实验室(哈尔滨师范大学)开放基金项目(ZK201204)，哈尔滨师范大学青年学术骨干资助计划项目(09KXQ-01)

Cloning and Expression Analysis of PtLIR1 Gene from Puccinellia tenuiflora Under NaCl Stress

Zhou Qi^1，2, Luo Chun², Guo Min², Fu Chang^1，2

1. KeyLaboratoryofPlantBiology，CollegeofHeilongjiangProvince，HarbinNormalUniversity，Harbin 150025；
2. College of Life Sciences and Technology，Harbin Normal University，Harbin 150025

Received:2015-05-06 Published:2015-11-26 Online:2015-11-26

摘要/Abstract

摘要： 类光诱导蛋白1基因LIR1与植物的抗逆性密切相关，可能与蔗糖水平的调节有关。克隆了星星草PtLIR1基因的编码区序列，在此基础上分析了NaCl胁迫下星星草根中PtLIR1的表达特性。PtLIR1基因的开放读码框全长408 bp，编码135个氨基酸。PtLIR1与黑麦草、水稻、玉米、芜青、小盐芥、拟南芥、短花药野生稻、粟、马铃薯、葡萄、二穗短柄草和大豆等的植物LIR1具有较高的同源性，其中与黑麦草类光诱导蛋白1的一致性最高，达80%。PtLIR1响应盐胁迫的实验表明，在盐胁迫早期，可溶性糖的积累与星星草根中PtLIR1基因的表达呈现出负相关性，在蔗糖水平的调节中其他蔗糖代谢相关基因可能发挥了更主要的作用。但在盐胁迫晚期，星星草根中的PtLIR1基因响应盐胁迫上调表达，可能在蔗糖水平的调节中发挥了主要作用。研究结果表明PtLIR1基因参与星星草根系对NaCl胁迫的应答，可能在星星草根抵御盐胁迫的过程中具有重要的调控作用。

关键词: 星星草, 类光诱导蛋白1, PtLIR1基因, 根, 盐胁迫

Abstract: Light regulated protein 1-like gene is closely associated with plant stress resistance and may mediated sucrose level. In this study, the open reading frame of PtLIR1 gene was cloned from Puccinellia tenuiflora, and its expression characteristics under NaCl stress were analyzed. The full length open reading frame of PtLIR1 was 408 bp, which encoded 135 amino acids. The deduced amino acid sequence of PtLIR1 gene shared high identity with its homologs from Oryza sativa, Zea mays, Brassica rapa, Thellungiella halophila, Arabidopsis thalian, Oryza brachyantha, Setaria italica, Solanum tuberosum, Vitis vinifera, Brachypodium distachyon, Glycine max, respectively, and showed the highest identity of 80% with Lolium perenne. At the early stage of salt stress, the expression of PtLIR1 was negatively correlated with the accumulation of soluble sugar, and the other sucrose metabolism related genes may play more important roles in the regulation of sucrose level. However, during the late stage of salt stress, PtLIR1 gene was up-regulated in Puccinellia tenuiflora roots and may play a vital role in regulating sucrose level. PtLIR1 gene was involved in response to NaCl stress, and maybe contribute to the salt tolerance of Puccinellia tenuiflora.

Key words: Puccinellia tenuiflora, light regulated protein 1-like protein, PtLIR1 gene, root, salt stress

周琪, 罗春, 郭敏, 付畅. 星星草PtLIR1基因克隆及其在NaCl胁迫下的表达分析[J]. 生物技术通报, 2015, 31(11): 166-172.

Zhou Qi, Luo Chun, Guo Min, Fu Chang. Cloning and Expression Analysis of PtLIR1 Gene from Puccinellia tenuiflora Under NaCl Stress[J]. Biotechnology Bulletin, 2015, 31(11): 166-172.

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星星草PtLIR1基因克隆及其在NaCl胁迫下的表达分析

Cloning and Expression Analysis of PtLIR1 Gene from Puccinellia tenuiflora Under NaCl Stress

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