miRNA qPCR检测方法研究进展及其应用

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.008

摘要/Abstract

摘要： miRNA是目前国际研究的热点领域之一，而miRNA表达检测是miRNA研究的基础内容。针对miRNA序列短小的特点，开发出了多种检测方法，其中miRNA qPCR定量检测是应用最广泛的方法。就miRNA抽提，miRNA qPCR定量检测原理及miRNA qPCR定量检测流程包括引物设计、反应体系设置、内参基因筛选等方面进行了深入探讨，并对miRNA定量检测研究方法的发展趋势进行了展望，以期为研究者进行miRNA qPCR定量检测提供参考。

关键词: miRNA, stem-loop RT-PCR, poly(A)加尾法RT-PCR, 延伸法RT-PCR, 数字PCR

Abstract: miRNAis one of thehottestresearch fields in the world.The detection of miRNA expression is the fundamental content in miRNA research.Due to the feature of miRNAs being short and small, varied methods were developed, and quantitative miRNA qPCR isthe most widely used one.This paper reviews thedevelopments of miRNA extraction method, quantitative detection principle of miRNAqPCR, primer design, the reaction condition, and reference gene selection.The developments ofmiRNA qPCR methods in the future are also discussed.All of these may provide a reference for researchers in the field when they detect miRNA by qPCR method.

Key words: miRNA, stem-loop RT-PCR, poly(A)tailing RT-PCR, extension RT-PCR, digital PCR

冯世鹏. miRNA qPCR检测方法研究进展及其应用[J]. 生物技术通报, 2016, 32(2): 59-69.

FENG Shi-peng. Research Advance on miRNA qPCR Methods and Its Application[J]. Biotechnology Bulletin, 2016, 32(2): 59-69.

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