生物技术通报 ›› 2016, Vol. 32 ›› Issue (5): 151-157.doi: 10.13560/j.cnki.biotech.bull.1985.2016.05.020

• 研究报告 • 上一篇    下一篇

一株碱蓬内生真菌的鉴定及促生活性产物的初步研究

于飞, 谷玥, 张奇, 卜宁   

  1. 沈阳师范大学化学与生命科学学院,沈阳 110034
  • 收稿日期:2015-09-09 出版日期:2016-05-25 发布日期:2016-05-27
  • 作者简介:于飞,女,硕士研究生,研究方向:资源与应用微生物;E-mail:qq-yf@126.com
  • 基金资助:
    国家自然科学基金资助项目(31270369),沈阳师范大学实验中心主任基金资助项目(SY201102)

The Identification of a Fungal Endophyte of Suaeda salsa and Preliminarily Research on Active Products of Promoting Growth

YU Fei,GU Yue,ZHANG Qi,BU Ning   

  1. College of Chemistry and Life Sciences,Shenyang Normal University,Shenyang 110034
  • Received:2015-09-09 Published:2016-05-25 Online:2016-05-27

摘要: 对辽宁盘锦红海滩的盐生碱蓬内生真菌JP4-1进行鉴定并研究其促生活性产物成分。根据形态学特征及18S rDNA序列分析对菌种进行鉴定,运用过滤离心、超声破碎、萃取、旋蒸等技术提取活性成分,并采用水培法研究该菌株发酵液对水稻幼苗的促生长活性。结果显示,经鉴定,JP4-1菌株为小丛壳属(Glomerella sp.),菌丝分枝,有隔,产孢;以正丁醇处理的JP4-1菌株胞外发酵液的小分子萃取物可使水稻幼苗的株高和叶片干重分别提高10.84%和14.67%,叶绿素含量提高44.22%,促生长效果明显。碱蓬内生真菌JP4-1的胞外发酵产物对水稻幼苗具有明显的促生长作用,其中以正丁醇萃取得到的小分子活性产物促生效果最为显著。

关键词: 碱蓬内生菌, 鉴定, 促生活性

Abstract: The purposes of this work are to identify the endophyte fungus JP4-1 of Suaeda salsa in Red Beach of Panjin,Liaoning Province,and to research the growth promotion activity of its metabolites. Endophytic fungus JP4-1 was identified using its morphological features and 18S rDNA,and several technical means such as filtering centrifuge,ultrasonic crushing,extraction,and rotary evaporateion were used to extract its active components,and hydroponic experiments were taken to test the growth promotion activity of its fermentation broth to rice seedlings. The JP4-1 was identified as Glomerella sp. with hyphae branched,septate,and producing spores. The result of hydroponic experiment revealed that the micromolecular products extracted from fermented liquid with n-butanol promoted the plant height 10.84%,dry weight of leaves 14.67%,and chlorophyll content 44.22% of the rice seedlings,i.e.,the growth promotion effects were remarkable. In conclusion,the extracellular fermentation products by the endophyte fungus JP4-1 of S. salsa significantly promoted the growth of rice seedlings,and the micromolecular active products extracted by n-butanol had the most significant promotion effects.

Key words: fungal endophyte, identification, growth promotion activity