结球甘蓝ARC1与Exo70A1编码区cDNA的克隆及进化分析

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.012

生物技术通报 ›› 2016, Vol. 32 ›› Issue (6): 76-88.doi: 10.13560/j.cnki.biotech.bull.1985.2016.06.012

结球甘蓝ARC1与Exo70A1编码区cDNA的克隆及进化分析

杨丹, 廉小平, 周燕, 张贺翠, 杜丹, 高启国, 任雪松, 朱利泉

西南大学农学部植物生理生化实验室，重庆 400715

收稿日期:2015-10-07 出版日期:2016-06-27 发布日期:2016-06-28
作者简介:杨丹，女，硕士研究生，研究方向：分子作物育种；E-mail：yangdan042561@163.com
基金资助:
国家自然科学基金项目(31572127)，重庆市自然基金重点项目(cstc2012jjB80010)

Cloning and Phylogenetic Analysis of cDNA Sequences Coding for ARC1 and Exo70A1 in Brassica oleraces var. capitata L.

YANG Dan, LIAN Xiao-ping, ZHOU Yan, ZHANG He-cui, DU Dan, GAO Qi-guo, REN Xue-song, ZHU Li-quan

Laboratory of Physiology and Biochemistry，Southwest University，Chongqing 400715

Received:2015-10-07 Published:2016-06-27 Online:2016-06-28

摘要/Abstract

摘要： 在甘蓝自交不亲和信号传导过程中，ARC1与Exo70A1的相互作用起着承上启下的关键作用。为了研究10种自交不亲和结球甘蓝材料ARC1和Exo70A1的进化关系，对其编码区cDNA进行了克隆和测序；在此基础上采用生物信息学方法，对这10种材料和前人已发表的83条ARC1和73条Exo70A1的相关序列的ARC1-Exo70A1的互作区和非互作区编码序列进行了变异和进化分析。结果发现：(1)所选育的10种结球甘蓝材料的ARC1与Exo70A1的编码区核酸序列存在平行进化关系，且均聚类于芸薹属分支下；(2)ARC1比Exo70A1编码区序列进化速率快；(3)ARC1和Exo70A1互作编码区核酸序列存在明显的协同进化，而ARC1和Exo70A1非互作编码区核酸序列则在进化上呈现明显分异；(4)ARC1和Exo70A1互作编码区cDNA的进化均快于非互作编码区。

关键词: ARC1, Exo70A1, 自交不亲和, 进化关系, 协同进化

Abstract: Interaction between ARC1 and Exo70A1 in Brassica is a key point of self-incompatibility(SI)signal transduction. In order to study the evolutionary relationship of ARC1 and Exo70A1 from 10 cultivars of SI head cabbages developed in recent years，we successfully cloned and sequenced cDNAs of ARC1 and Exo70A1. Furthermore，by bioinformatics，we had mutation and evolutionary analysis of those coding region sequences，including interaction region and non-interaction regions of ARC1-Exo70A1 from the 10 cabbages and prior published 83 ARC1 and 73 Exo70A1 sequences. The results revealed that：1)The coding nucleic acid sequences of ARC1 and Exo70A1 from the 10 cabbage materials presented parallel evolutionary relationships，and belonged to the Brassica branch. 2)The evolution rate of coding sequence of ARC1 was faster than Exo70A1. 3)The interaction coding region of ARC1-Exo70A1 was obviously in the progress of co-evolution，while the non-interaction coding area of ARC1-Exo70A1 showed significant differentiation rate in evolution. 4)The evolutionary rate of interaction coding area was faster than non-interaction coding region.

Key words: ARC1, Exo70A1, self-incompatibility, evolutionary relationship, co-evolution

杨丹, 廉小平, 周燕, 张贺翠, 杜丹, 高启国, 任雪松, 朱利泉. 结球甘蓝ARC1与Exo70A1编码区cDNA的克隆及进化分析[J]. 生物技术通报, 2016, 32(6): 76-88.

YANG Dan, LIAN Xiao-ping, ZHOU Yan, ZHANG He-cui, DU Dan, GAO Qi-guo, REN Xue-song, ZHU Li-quan. Cloning and Phylogenetic Analysis of cDNA Sequences Coding for ARC1 and Exo70A1 in Brassica oleraces var. capitata L.[J]. Biotechnology Bulletin, 2016, 32(6): 76-88.

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结球甘蓝ARC1与Exo70A1编码区cDNA的克隆及进化分析

Cloning and Phylogenetic Analysis of cDNA Sequences Coding for ARC1 and Exo70A1 in Brassica oleraces var. capitata L.

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