过表达VHA-c3基因拟南芥对黑暗、ABA与糖的响应

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.013

生物技术通报 ›› 2016, Vol. 32 ›› Issue (6): 89-95.doi: 10.13560/j.cnki.biotech.bull.1985.2016.06.013

过表达VHA-c3基因拟南芥对黑暗、ABA与糖的响应

苏杰^1,2, 郭荣起^2,3, 李国婧², 王瑞刚²

1. 内蒙古农业大学职业技术学院，土右旗 014109
2. 内蒙古农业大学生命科学学院，呼和浩特 010018
3. 内蒙古呼伦贝尔市农业科学研究所，牙克石 162650

收稿日期:2015-09-14 出版日期:2016-06-27 发布日期:2016-06-28
作者简介:苏杰，女，硕士，讲师，研究方向：植物生物化学与分子生物学；E-mail：sujie_1546@163.com
基金资助:
国家自然科学基金项目(30460018)，内蒙古自治区科技创新团队(201503004)

Responses of Overexpressed Arabidopsis VHA-c3 to Dark，ABA and Sugar

SU Jie^1，2, GUO Rong-qi^2，3, LI Guo-jing², WANG Rui-gang²

1. School of Vocational and Technical, Inner Mongolia Agricultural University, Tuyouqi 014109
2. School of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018
3. Agricultural Sciences of Hulunbeier in Inner Mongolia，Yakeshi 162650

Received:2015-09-14 Published:2016-06-27 Online:2016-06-28

摘要/Abstract

摘要： 为研究液泡H⁺-ATPase c亚基基因(VHA-c3)在植物生长发育及非生物胁迫应答过程中的作用，构建了VHA-c3过表达载体转化拟南芥，获得过表达VHA-c3的转基因纯合体植株，采用半定量RT-PCR技术分析了转基因拟南芥中VHA-c3的表达量，然后对转基因拟南芥进行暗培养、ABA和糖处理。结果获得6个T2代株系转基因纯合体株系，其mRNA表达量均高于对照；黑暗条件下，5个VHA-c3转基因株系的根长变短；在正常光照下，3个转基因株系主根伸长和子叶的展开以及5个转基因株系的种子萌发对ABA的抑制不敏感；分别有5个和6个转基因株系的种子萌发对葡萄糖和蔗糖的抑制不敏感。推测VHA-c3可能影响根细胞的扩展，并可能参与ABA和糖介导的信号转导途径。

关键词: 拟南芥, VHA-c3, 黑暗, ABA, 糖

Abstract: For studying the role of vacuole H⁺-ATPase subunit c gene(VHA-c3)in plant growth and abiotic stress response，an over-expression vector of VHA-c3 was constructed and introduced into wild-type Arabidopsis thaliana. The expression levels of VHA-c3 in the transgenic homozygote’s plants were detected by semi-quantitative RT-PCR，then the transgenic homozygote were treated by dark，ABA and sugar. The results indicated that 6 lines of homozygous T2 transgenic plants were obtained，and their corresponding transcript levels were increased. The root lengths of 5 lines were reduced among the dark-grown seedlings. Among the ABA-treated seedlings，3 lines were insensitive in primary root growth and the extent of cotyledon unfolding，and 5 lines were insensitive in seed germination. When treated by glucose and sucrose，5 lines and 6 lines reduced the sensitivity in seed germination，respectively. These results indicate that VHA-c3 may affect the cell expansion of root，and be involved in the signal transduction pathways mediated by ABA and sugar.

Key words: Arabidopsis thaliana, VHA-c3, dark, ABA, sugar

苏杰, 郭荣起, 李国婧, 王瑞刚. 过表达VHA-c3基因拟南芥对黑暗、ABA与糖的响应[J]. 生物技术通报, 2016, 32(6): 89-95.

SU Jie, GUO Rong-qi, LI Guo-jing, WANG Rui-gang. Responses of Overexpressed Arabidopsis VHA-c3 to Dark，ABA and Sugar[J]. Biotechnology Bulletin, 2016, 32(6): 89-95.

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过表达VHA-c3基因拟南芥对黑暗、ABA与糖的响应

Responses of Overexpressed Arabidopsis VHA-c3 to Dark，ABA and Sugar

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