Dof转录因子在植物中的调控作用

doi:10.13560/j.cnki.biotech.bull.1985.2018-1062

摘要/Abstract

摘要： DNA binding with one finger（Dof）蛋白是植物中特有的一类转录因子,自1993年在玉米中发现该转录因子到如今已有26年的历史。Dof蛋白只有一个由52个氨基酸组成的单锌指保守结构域,该结构域包含4个保守的半胱氨酸残基和一个二价锌离子,这两者都是Dof转录因子重要的组成部分,二者缺一不可,若半胱氨酸发生突变,或存在其他二价离子螯合剂都将会影响锌指结构的生成,进而使Dof蛋白不能与DNA结合,导致其丧失活性。半胱氨酸残基和Zn²⁺可以共价结合构成CX2CX21CX2C基序,此基序能够特异识别启动子中的AAAG（或CTTT）序列,从而激活或抑制靶基因的表达。Dof蛋白C-末端氨基酸序列的多变性导致该转录因子进化的多样性,从而在植物的生长发育过程中发挥着不同且重要的功能,如种子萌发、维管的发育和叶片极性、花和花粉发育、光周期反应、参与碳和氮的代谢、合成次生代谢产物、植物的逆境响应过程等。围绕着Dof转录因子的结构、系统进化关系、motif的组成和在植物中发挥的功能展开综述,以期为后续Dof转录因子的研究奠定相应的理论基础。

关键词: Dof转录因子, 单锌指保守结构域, 进化关系, 调控, 代谢

Abstract: As a plant-specific transcription-factor,DNA binding with one finger（Dof）protein was firstly discovered in corn in 1993. Dof has only one conserved zinc finger domain containing 52 amino acids. In general,the zinc finger domain contains 4 conserved Cys residues and one Zn²⁺,and these two components are both indispensable for Dof protein. Cysteine mutation or the presence of a divalent ion chelating agent will affect the formation of the zinc finger structure,and then the Dof protein cannot bind to the target DNA,resulting in the loss of its activity. The Cys residue and Zn²⁺ form a CX2CX21CX2C motif by covalent binding of other amino acid residues,and this motif may specifically recognize AAAG（or CTTT）sequences in promoters,thus activating or inhibiting the expression of target genes. Besides,the C-terminal amino acid sequence of the Dof protein was volatile,which led to the evolutionarily diverse and functional diversity of Dof transcription factor. To date,the Dof transcription factor was reported to play an important role in many aspects of plant growth and development,such as seed germination,development of vascular,leaf polarity,development of flower and pollen,photoperiod reaction,metabolism of carbon and nitrogen,regulation of secondary metabolites,and stress responses. This review focuses on the structure,phylogenetic relationship,and motif composition and regulation metabolism of Dof transcription factors,aiming at providing a theoretical basis for the further study of Dof transcription factors.

Key words: Dof transcription factor, conserved domain of single zinc finger, evolutionary relationship, regulate and control, metabolism

郭彦秀, 陈静, 王艳芳, 孙春玉, 王义, 张美萍. Dof转录因子在植物中的调控作用[J]. 生物技术通报, 2019, 35(5): 146-156.

GUO Yan-xiu, CHEN Jing, WANG Yan-fang, SUN Chun-yu, WANG Yi, ZHANG Mei-ping. Roles of Dof Transcription Factors in the Regulation of Plant[J]. Biotechnology Bulletin, 2019, 35(5): 146-156.

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