拮抗多种植物病原真菌Streptomyces triostinicus C2的分离与鉴定

doi:10.13560/j.cnki.biotech.bull.1985.2016.07.016

生物技术通报 ›› 2016, Vol. 32 ›› Issue (7): 106-111.doi: 10.13560/j.cnki.biotech.bull.1985.2016.07.016

拮抗多种植物病原真菌Streptomyces triostinicus C2的分离与鉴定

彭卫福¹, 吴志明², 陈未², 曾勇军¹, 李昆太²

1. 江西农业大学作物生理生态与遗传育种教育部重点实验室江西省作物生理生态与遗传育种重点实验室,南昌 330045；
2. 江西农业大学生物科学与工程学院江西省农业微生物资源开发与利用工程实验室,南昌 330045

收稿日期:2015-10-30 出版日期:2016-07-25 发布日期:2016-07-25
作者简介:彭卫福,男,博士研究生,研究方向：农业微生物;E-mail：pengwei_fu@126.com
基金资助:
国家科技支撑计划项目（2011BAD16B0402,2013BAD07B12）,江西省青年科学家培养对象计划项目（20142BCB23025）,国家级大学生创新创业训练计划项目（201410410008）

Isolation and Identification of Streptomyces triostinicus C2 Antagonizing on a Variety of Plant Pathogenic Fungi

PENG Wei-fu¹, WU Zhi-ming², CHEN Wei², ZENG Yong-jun¹, LI Kun-tai²

1. Key Laboratory of Crop Physiology,Ecology and Genetic Breeding of Ministry of Education,Key Laboratory of Crop Physiology,Ecology and Genetic Breeding of Jiangxi Province,Jiangxi Agriculture University,Nanchang 330045;
2. Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources,College of Biological Sciences and Technology,Jiangxi Agriculture University,Nanchang 330045

Received:2015-10-30 Published:2016-07-25 Online:2016-07-25

摘要/Abstract

摘要： 旨在分离筛选获得对植物病原真菌具有抑制作用的拮抗菌。以水稻纹枯病菌为指示菌,采用平板对峙培养法进行拮抗菌的分离筛选;根据菌体形态学观察、生理生化特征以及16S rDNA序列分析,对拮抗菌进行菌种鉴定。结果显示,从采集自云南、广东、安徽、湖北、江西等地的18个土样中分离筛选到一株对水稻纹枯病菌具有良好抑制效果的菌株C2,该菌株初步鉴定为Streptomyces triostinicus,并暂命名为链霉菌 C2;进一步测定链霉菌C2的抗菌谱发现,它对水稻纹枯病菌、水稻稻瘟病菌、葡萄炭疽病菌、西瓜枯萎病菌和橘青霉等多种植物病原真菌均具有良好的抑制作用,其抑菌率分别为49.78%、56.62%、80.96%、18.59%和94.10%。链霉菌C2是一株植物病原真菌广谱拮抗菌。

关键词: 分离与鉴定, 放线菌菌株C2, 植物病原真菌, 广谱拮抗

Abstract: The research purposes of this work are to isolate and screen a strain against phytopathogenic fungi. The antagonistic strain was isolated and screened using plate confrontation culture with Rhizoctonia solani as indicator,and was further identified on the basis of morphological characteristics,physiological and biochemical characterization,and 16S rDNA sequence analysis. From 18 soil samples collected from Yunnan,Guangdong,Anhui,Hubei,and Jiangxi Provinces of China,the isolated actinomycete strain C2 presented a strong inhibitory effect on R. solani,and it was preliminarily identified as Streptomyces triostinicus,designated as Streptomyces strain C2 here. The bioassay results showed that Streptomyces strain C2 possessed a broad-spectrum inhibitory effect on a range of plant fungi such as R. solani,Pyricularia grisea,Colletotrichum gloeosporioides,Fusarium oxysporum f. sp. niveum,and Penicillium citrinum,and the inhibition percentage was 49.78%,56.62%,80.96%,18.59%,and 94.10%,respectively. Conclusively,Streptomyces strain C2 was an antagonistic strain with broad-spectrum in suppressing the growth of various plant fungal pathogens.

Key words: isolation and identification, actinomycete strain C2, phytopathogenic fungi, broad-spectrum antagonist

彭卫福, 吴志明, 陈未, 曾勇军, 李昆太. 拮抗多种植物病原真菌Streptomyces triostinicus C2的分离与鉴定[J]. 生物技术通报, 2016, 32(7): 106-111.

PENG Wei-fu, WU Zhi-ming, CHEN Wei, ZENG Yong-jun, LI Kun-tai. Isolation and Identification of Streptomyces triostinicus C2 Antagonizing on a Variety of Plant Pathogenic Fungi[J]. Biotechnology Bulletin, 2016, 32(7): 106-111.

参考文献

[1] Chakraborty S, Newton AC. Climate change, palnt diseases and food security：an over review[J]. Plant Pathology, 2011, 60：2-14.
[2] 康振生. 我国植物真菌病害的研究现状及发展策略[J]. 植物保护, 2010, 36（3）：9-12.
[3] Berendsen RL, Pieterse CM, Bakker PAHM. The rhizosphere microbiome and plant health[J]. Trends Plant Sci, 2012, 17：478-486.
[4] Doornbos RF, van Loon LC, Bakker PAHM. Impact of root exudates and plant defense signaling on bacterial communities in the rhizosphere. A review[J]. Agron Sustain Dev, 2012, 32：227-243.
[5] Xiong ZQ, Tu XR, Wei SJ, et al. In vitro antifungal activity of antifungalmycin 702, a new polyene macrolide antibiotic, against the rice blast fungus Magnaporthe grisea[J]. Biotechnol Lett, 2013, 35：1475-1479.
[6] Harikrishnan H, Shanmugaiah V, Balasubramanian N, et al. Antagonistic potential of native strain Streptomyces aurantiogriseus VSMGT1014 against sheath blight of rice disease[J]. World J Microbiol Biotechnol, 2014, 30：3149-3161.
[7] Summerell BA, Laurence MH, Liew EC, et al. Biogeography and phylogeography of Fusarium：a review[J]. Fungal Divers, 2010, 44：3-13.
[8] Zhao S, Liu DY, Ling N, et al. Bio-organic fertilizer application significantly reduces the Fusarium oxysporum population and alters the composition of fungi communities of watermelon Fusarium wilt rhizosphere soil[J]. Biol Fert Soils, 2014, 50：765-774.
[9] Li CH, Zhao MW, Tang CM, et al. Population dynamics and identification of endophytic bacteria antagonistic toward plant-pathogenic fungi in cotton root[J]. Microbial Ecol, 2010, 59：344-356.
[10] Araújo L, Gonçalves AE, Stadnik MJ. Ulvan effect on conidial germination and appressoria formation of Colletotrichum gloeosporioides[J]. Phytoparasitica, 2014, 42：631-640.
[11] 彭卫福, 李昆太, 曾勇军. 水稻病害的微生物防治研究进展[J]. 江西农业大学学报, 2015, 37（4）：625-631.
[12] Compant S, Brader G, Muzammil S, et al. Use of beneficial bacteria and their secondary metabolites to control grapevine pathogen diseases[J]. BioControl, 2013, 58（4）：435-455.
[13] Shirling EB, Gottlieb D. Methods for characterization of Streptom-yces species[J]. Int Journal of Syst Bacteriol, 1966, 16（3）：313-340.
[14] 中国科学院微生物研究所放线菌分类组. 链霉菌鉴定手册[M]. 北京：科学出版社, 1975.
[15] 余佳清, 张志斌, 李希茜, 等. 一株具有抗真菌活性的放线菌菌株FXj9的分离和鉴定[J]. 植物保护, 2013, 39（5）：158-164.
[16] 阎逊初. 放线菌的分类和鉴定[M]. 北京：科学出版社, 1992.
[17] Weller DM, Raaijmakers JM, Gardener BBM, et al. Microbial populations responsible for specific soil suppressiveness to plant pathogens 1[J]. Annu Rev Phytopathol, 2002, 40：309-348.
[18] Sivasakthi S, Usharani G, Saranraj P. Biocontrol potentiality of plant growth promoting bacteria（PGPR）-Pseudomonas fluorescens and Bacillus subtilis：A review[J]. African Journal of Agricultural Research, 2014, 9（16）：1265-1277.
[19] Couillerot O, Loqman S, Toribio A, et al. Purification of antibiotics from the biocontrol agent Streptomyces anulatus S37 by centrifugal partition chromatography[J]. J Chromatogr B, 2014, 944：30-34.
[20] Kim BS, Hwang BK. Microbial fungicides in the control of plant diseases[J]. J Phytopathology, 2007, 155：641-653.
[21] 曹琦琦, 周登博, 郑丽, 等. 水稻纹枯病菌拮抗菌的筛选、鉴定及培养条件探索[J]. 中国生物防治学报, 2013, 29（2）：270-276.
[22] Glund K, Schlumbohm W, Bapat M, et al. Biosynthesis of quinoxaline antibiotics：purification and characterization of the quinoxaline-2-carboxylic acid activating enzyme from Streptomyces triostinicus[J]. Biochemistry, 1990, 29（14）：3522-3527.
[23] Praseuth AP, Wang CCC, Watanabe K, et al. Complete sequence of biosynthetic gene cluster responsible for producing triostin A and evaluation of quinomycin-type antibiotics from Streptomyces triostinicus[J]. Biotechnol Prog, 2008, 24（6）：1226-1231.
[24] Sato M, Nakazawa T, Tsunematsu Y, et al. Echinomycin biosynthesis[J]. Curr Opin Chem Biol, 2013, 17（4）：537-545.
[25] Singh V, Khan M, Khan S, et al. Optimization of actinomycin V production by Streptomyces triostinicus using artificial neural network and genetic algorithm[J]. Appl Microbiol Biot, 2009, 82（2）：379-385.

拮抗多种植物病原真菌Streptomyces triostinicus C2的分离与鉴定

Isolation and Identification of Streptomyces triostinicus C2 Antagonizing on a Variety of Plant Pathogenic Fungi

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