温度对裂片石莼生长及叶绿素荧光特性的影响

doi:10.13560/j.cnki.biotech.bull.1985.2016.07.015

摘要/Abstract

摘要： 旨在研究裂片石莼（Ulva fasciata Delile）对温度适应机制,将藻体于5℃、10℃、15℃、20℃、25℃、30℃、33℃、35℃培养7 d,再经过25℃培养2 d,通过测定不同温度下裂片石莼的生长变化、叶绿素含量、叶绿素荧参数及25℃再培养后叶绿素荧光参数探讨不同温度对裂片石莼的影响。结果显示,25℃培养的藻体的生长、叶绿素含量、叶绿素荧光参数（F_v/F_m、F_v'/F_m'、α、qP）等最大;随着温度的升高或者降低,各项生理指标逐渐降低,而非光化学淬灭系数NPQ随着温度增大或者降低逐渐升高,10℃培养最大,5℃、35℃条件下的各项生理指标最低。5℃、10℃、15℃、20℃、30℃下的藻体经过25℃培养2 d后F_v/F_m、F_v'/F_m'都有所提高,10℃培养后的藻体提高最大,33℃、35℃再培养后F_v/F_m、F_v'/F_m'几乎没有变化。研究表明,裂片石莼生长和光合作的最适温度范围为15℃-30℃,5℃和35℃分别为其最高和最低耐受温度,裂片石莼对低温的耐受性大于高温。

关键词: 裂片石莼, 温度, 生长, 叶绿素荧光

Abstract: In order to study the temperature adaptation mechanism of Ulva fasciata Delile,culturing the U. fasciata under the temperatures：5℃,10℃,15℃,20℃,25℃,30℃,33℃ and 35℃ for 7 d,and the growth change,chlorophyll content,and indexes of chlorophyll fluorescence of U. fasciata at different temperatures as well as the parameters of chlorophyll fluorescence after re-culturing at 25℃ were examined to understand the effects of different temperatures on the U. fasciata. Results showed that growth rate,chlorophyll content,the indexes of fluorescence（optimal photo-chemical quantum yield（F_v/F_m）,effective optimal photo-chemical quantum yield（F_v'/F_m'）,photosynthetic light use efficiency（α）,and photo-chemical quenching（qP））all reached the maximum at 25℃. All these physiological indexes of the seaweeds decreased gradually when the temperature were higher or lower than 25℃,and the lowest at 5℃ and 35℃;however,the non-photo-chemical quenching（NPQ）gradually rose with the increase or decrease of temperature,and till its maximum value at 10℃. The F_v/F_m and F_v'/F_m' values of the samples of 5℃ to 30℃ groups increased a bit after they were recovered at 25℃ for 2 d,and the highest increment occurred in 10℃ group. Meanwhile,the indexes did not change in the group of 33℃ and 35℃ after recovered cultivation. The results indicated that the most appropriate temperature for U. fasciata to grow and photo-synthesis was in a range of 15℃ to 30℃,5℃and 35℃ were the lowest and highest growth temperature respectively,and U. fasciata showed greater tolerance to low temperature than to high temperature.

Key words: Ulva fasciata, temperature, growth, chlorophyll fluorescence

黄艳花, 杨锐, 孙庆海. 温度对裂片石莼生长及叶绿素荧光特性的影响[J]. 生物技术通报, 2016, 32(7): 99-105.

HUANG Yan-hua, YANG Rui, SUN Qing-hai. Effect of Temperature on Growth and Chlorophyll Fluorescence of Ulva fasciata[J]. Biotechnology Bulletin, 2016, 32(7): 99-105.

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