基于高通量测序技术分析糖尿病与肠道菌群相关性究进展

doi:10.13560/j.cnki.biotech.bull.1985.2016.09.009

摘要/Abstract

摘要： 肠道菌群与糖尿病的发生发展密切相关,随着高通量测序技术的发展,越来越多的研究表明,糖尿病状态下,宿主肠道菌群的群落结构发生了变化。综述了国际上利用高通量技术研究的糖尿病状态下肠道菌群的差异的最新进展,同时从食物、药物、益生元和粪便菌群移植等方面阐述肠道菌群对糖尿病的调节机制,旨在为预防和治疗糖尿病提供新的思路。

关键词: 糖尿病, 高通量测序, 肠道菌群

Abstract: There is a metabolic link between gut microbiota and Diabetes mellitus. With the development of high throughput technologies, more and more studies show that the community structure of the gut microbiota has changed in the diabetic state. This paper reviewed the latest progress on the formation of the differences of gut microbiota in the diabetic state by the application of high throughput technologies,and also the regulatory mechanisms of gut microbiota on D. mellitus were explained from the aspects of food,drugs,prebiotics and fecal transplants.This paper provided a more methodical idea for prevention and treatment of D. mellitus.

Key words: Diabetes mellitus, high throughput sequencing, gut microbiota

刘冬恋, 廖梦玲, 周欢. 基于高通量测序技术分析糖尿病与肠道菌群相关性究进展[J]. 生物技术通报, 2016, 32(9): 59-64.

LIU Dong-lian, LIAO Meng-ling, ZHOU Huan. Research Progress on the Correlation Analysis Between Diabetes and Gut Microbiota Using High Throughput Sequencing[J]. Biotechnology Bulletin, 2016, 32(9): 59-64.

参考文献

[1] 郑艺, 张家超, 郭壮, 等. 基于高通量测序技术分析肠道菌群及其影响因素的研究进展[J]. 中国食品学报, 2014, 14（11）：157-165.
[2] 周雪雁, 刘翊中, 陈轶霞, 等. 动物肠道菌群结构分析方法进展[J]. 微生物学杂志, 2013, 33（5）：81-87.
[3] 李东萍, 郭明璋, 许文涛. 16S rRNA测序技术在肠道微生物中的应用研究进展[J]. 生物技术通报, 2015, 31（2）：71-77.
[4] Sekirov I, Russell SL, Antunes LC, et al. Gut microbiota in health and disease[J]. Physiolog Rev, 2010, 90（3）：859-904.
[5] 谷莉. 不同鼠种的肠道菌群在不同饮食结构干预中的组成改变[D]. 长沙：中南大学, 2014.
[6] 齐翠娟, 肖新华. 肠道菌群与1型糖尿病[J]. 中国糖尿病杂志, 2015, 23（6）：574-576.
[7] Alkanani AK, Hara N, Gottlieb PA, et al. Alterations in intestinal microbiota correlate with susceptibility to type 1 diabetes[J]. Diabetes, 2015, 64（10）：3510-3520.
[8] Mejía-León ME, Petrosino JF, Ajami NJ, et al. Fecal microbiota imbalance in Mexican children with type 1 diabetes[J]. Sci Rep, 2014, 4：3814.
[9] Wirth R, Bódi N, Maróti G, et al. Regionally distinct alterations in the composition of the gut microbiota in rats with streptozotocin-induceddiabetes[J]. PLoS One, 2014, 9（12）：110440.
[10] Patterson E, Marques TM, O’Sullivan O, et al. Streptozotocin-induced type-1-diabetes disease onset in Sprague-Dawley rats is associated with an altered intestinal microbiota composition and decreased diversity[J]. Microbiology, 2015, 161（1）：182-193.
[11] Qin J, Li Y, Cai Z, et al. A metagenome-wide association study of gut microbiota in type 2 diabetes[J]. Nature, 2012, 490（7418）：55-60.
[12] Vital M, Howe AC, Tiedje JM, et al. Revealing the bacterial butyrate synthesis pathways by analyzing（meta）genomic data[J]. MBio, 2014, 5（2）：00889.
[13] Larsen N, Vogensen FK, van den Berg FW, et al. Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults[J]. PLoS One, 2010, 5（2）：e9085.
[14] Zhang X, Shen D, Fang Z, et al. Human gut microbiota changes reveal the progression of glucose intolerance[J]. PLoS One, 2013, 8（8）：e71108.
[15] Fugmann M, Breier M, Rottenkolber M, et al. The stool microbiota of insulin resistant women with recent gestational diabetes, a high risk group for type 2 diabetes[J]. Sci Rep, 2015, doi:10. 1038/srep13212.
[16] Hu J, Nomura Y, Bashir A, et al. Diversified microbiota of meconium is affected by maternal diabetes status[J]. PLoS One, 2013, 8（11）：78257.
[17] Di Luccia B, Crescenzo R, Mazzoli A, et al. Rescue of fructose-induced metabolic syndrome by antibiotics or faecal transplantation in a rat model of obesity[J]. PLoS One, 2015, 10（8）：0134893.
[18] Hansen CH, Krych L, Buschard K, et al. A maternal gluten-free diet reduces inflammation and diabetes incidence in the offspring of NOD mice[J]. Diabetes, 2014, 63（8）：2821-2832.
[19] Marietta EV, Gomez AM, Yeoman C, et al. Low incidence of spontaneous type 1 diabetes in non-obese diabetic mice raised on gluten-free diets is associated with changes in the intestinal microbiome[J]. PLoS One, 2013, 8（11）：e78687.
[20] Kim MS, Hwang SS, Park EJ, et al. Strict vegetarian diet improves the risk factors associated with metabolic diseases by modulating gut microbiotaand reducing intestinal inflammation[J]. Environ Microbiol Rep, 2013, 5（5）：765-775.
[21] Su B, Liu H, Li J, et al. Acarbose treatment affects the serum levels of inflammatory cytokines and the gut content of bifidobacteria in Chinese patients with type 2 diabetes mellitus 2[J]. J Diabetes, 2015, 7（5）：729-739.
[22] Shin NR, Lee JC, Lee HY, et al. An increase in the Akkermansia spp. population induced by metformin treatment improves glucose homeostasis in diet-induced obese mice[J]. Gut, 2014, 63（5）：727-735.
[23] Xu J, Lian F, Zhao L, et al. Structural modulation of gut microbiota during alleviation of type 2 diabetes with a Chinese herbal formula[J]. ISME J, 2015, 9（3）：552-562.
[24] Zhang X, Zhao Y, Zhang M, et al. Structural changes of gut microbiota during berberine-mediated prevention of obesity and insulin resistance in high-fat diet-fed rats[J]. PLoS One, 2012, 7（8）：42529.
[25] Anhe FF, Roy D, Pilon G, et al. A polyphenol-rich cranberry extract protects from diet-induced obesity, insulin resistance and intestinal inflammation in association with increased Akkermansia spp. population in the gut microbiota of mice[J]. Gut, 2015, 64（6）：872-883.
[26] Roopchand DE, Carmody RN, Kuhn P, et al. Dietary polyphenols promote growth of the gut bacterium Akkermansia muciniphila and attenuate high-fat diet-induced metabolic syndrome[J]. Diabetes, 2015, 64（8）：2847-2858.
[27] Schogor AL, Huws SA, Santos GT, et al. Ruminal Prevotella spp. may play an important role in the conversion of plant lignans into human health beneficial antioxidants[J]. PLoS One, 2014, 9（4）：87949.
[28] 沈定树, 郑静. 益生元与肠道微生态[J]. 中国微生态学杂志, 2013, 25（6）：742-744.
[29] Everard A, Lazarevic V, Derrien M, et al. Responses of gut microbiota and glucose and lipid metabolism to prebiotics in genetic obese and diet-induced leptin-resistant mice[J]. Diabetes, 2011, 60（11）：2775-2786.
[30] Dewulf EM, Cani PD, Claus SP, et al. Insight into the prebiotic concept：lessons from an exploratory, double blind intervention study with inulin-type fructans in obese women[J]. Gut, 2013, 62（8）：1112-1121.
[31] 张发明, 范志宁, 季国忠. 粪菌移植的概念、历史、现状和未来[J]. 中国内镜杂志, 2012, 18（9）：930-935.
[32] Brown K, Godovannyi A, Ma C, et al. Prolonged antibiotic treatment induces a diabetogenic intestinal microbiome that accelerates diabetes in NOD mice[J]. ISME J, 2015, 10（2）：321-322.
[33] Hartstra AV, Bouter KE, Backhed F, et al. Insights into the role of the microbiome in obesity and type 2 diabetes[J]. Diabetes Care, 2015, 38（1）：159-165.
[34] Candon S, Perez-Arroyo A, Marquet C, et al. Antibiotics in early life alter the gut microbiome and increase disease incidence in a spontaneous mouse model of autoimmune insulin-dependent diabetes[J]. PLoS One, 2015, 10（5）：e0125448.
[35] 叶乃芳, 王中新. 16S rRNA及相关技术用于临床细菌鉴定的研究进展[J]. 国际检验医学杂志, 2015, 36（4）：520-522.