关键词: 酿酒酵母, 3-（4-羟基苯基）丙酸（HPPA）, 生物合成, 酪氨酸氨基转移酶, 对香豆酰辅酶A连接酶?

Abstract: 3-（4-Hydroxyphenyl）propionic acid（HPPA）is an aromatic compound with importantly economic value,because it is an intermediate mainly used in medicine,food and chemical industry. In this work,we aim to achieve the De novo synthesis of HPPA in Saccharomyces cerevisiae,and it was conducted via overexpressing a tyrosine ammonia-lyase from Flavobacterium johnsoniaeu（FjTAL）and a p-coumaroyl-CoA ligase from Arabidopsis thaliana（At4CL）,as well as using super long-chain enoyl-CoA reductase（ScTSC13）and acyl-CoA thioesterases from S. cerevisiae BY4742. The yield of HPPA reached 70 mg/L. Bioconversion rate of tyrosine into p-coumaric acid was 36% under the condition of 4 mmol/L tyrosine enhancement in shake flask after 72 h fermentation at 30℃ and galactose inducing FjTAL overexpression. While in the recombinant S. cerevisiae overexpressing At4CL combined with enzymes ScTSC13 and acyl-CoA thioesterases,94% of 4 mmol/L p-coumaric acid was converted to HPPA in shake flask after 72 h fermentation at 30℃,and 91 % of 4 mM caffeic acid was converted to DHPPA. This work laid a foundation for the biosynthesis of HPPA and its derivatives.

Key words: Saccharomyces cerevisiae, 3-（4-hydroxyphenyl）propionic acid, biosynthesis, tyrosine ammonia-lyase, p-coumaroyl-coA ligase