植物核苷二磷酸激酶研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2017-0261

摘要/Abstract

摘要： 核苷二磷酸激酶（Nucleoside diphosphate kinases,NDPKs）是一类高度保守的蛋白,大小一般在70-100 kD,在生物体内大多数以六聚体形式存在,仅在少数原核生物中以四聚体形式存在。NDPK主要参与维持核苷二磷酸和核苷三磷酸的平衡。目前在植物中已发现4种NDPK：NDPKⅠ、NDPKⅡ、NDPKⅢ和NDPKⅣ,相关研究主要集中在前3种。NDPKⅠ与植物生长发育、非生物胁迫、感病应激和激素响应有关;NDPKⅡ参与光合作用和活性氧清除;NDPKⅢ参与能量代谢和细胞程序性死亡;NDPKⅣ仅在拟南芥和水稻基因组中发现,预测定位于内质网,功能未知。除了上述的主要作用外,NDPK在某些植物中还有特殊功能,如参与DNA复制、参与淀粉和纤维素的合成、参与生长素调节和发挥核酶活性等。这些作用机制是否存在物种特异性还有待进一步的研究。对NDPK的系统进化、生物功能的最新进展进行了综述。最后对NDPK的发展趋势进行了展望,有助于将来对NDPK进行更深入和全面的研究。

关键词: 核苷二磷酸激酶, NDPK, 系统进化, 功能

Abstract: Nucleoside diphosphate kinases（NDPKs）are a class of highly conserved proteins with a size between 70 to 100 kD. They exist as hexamers in most organisms while as tetramers only in few prokaryotes. NDPKs are mainly responsible for keeping the in vivo cellular balance between nucleoside diphosphates and nucleoside triphosphates. Four types of NDPKs,i.e.,NDPKⅠ,NDPKⅡ,NDPKⅢ and NDPKⅣ,are found in plants,however,till now researches have been mainly focused on the first three. NDPKIs are found to be involved in plant growth and development,and response to abiotic stress,pathogens and hormones. NDPKⅡs take part in photosynthesis and removal of reactive oxygen species. NDPKIIIs are involved in energy metabolism and programmed cell death. NDPKIVs are only found in Arabidopsis and rice genomes and predicted to be located in endoplasmic reticulum with unknown function. Besides,NDPKs also display special functions in certain plants,such as DNA replication,starch and cellulose synthesis,auxin signaling and ribozyme activities;however,whether these functions are species-specific needs further study. This article summarizes the phylogenetic analysis and the most recent research progress on biological functions of NDPKs. Finally,the article discusses the prospect of NDPKs research,thus it will facilitate the in-depth and comprehensive study of their function in plants in the future.

Key words: nucleoside diphosphate kinase, NDPK, phylogeny analysis, function

朱馨妮, 汪珊珊, 周佳琴, 朱世华. 植物核苷二磷酸激酶研究进展[J]. 生物技术通报, 2017, 33(11): 19-28.

ZHU Xin-ni, WANG Shan-shan, ZHOU Jia-qin, ZHU Shi-hua. Research Advances of NDPKs in Plants[J]. Biotechnology Bulletin, 2017, 33(11): 19-28.

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