关键词: 蜚蠊, 放线菌, 抗真菌活性, 分类鉴定

Abstract: This study aims to screen and identify actinomycetes strains with antifungal activity from guts of Periplaneta americana. Antifungal activities of 159 actinomycetes strains of Periplaneta americana were screened using the Oxford cup and confrontation co-culture method in vitro while using 4 species of human pathogenic fungi of Candida albicans,Trichophyton rubrum,Aspergillus niger,and Aspergillus fumigatus as the research object. Subsequently 16S rDNA PCR,Blast homology alignment and phylogenetic tree were used to identify target strains. Results showed that 45 actinomycetes had antifungal activity,many of them had a wide antifungal spectrum,and 10 actinomycetes strains were antagonistic to all 4 tested fungi. There were 32 strains Streptomyces that were dominant actinomycetes among them,accounting for 71.11%;6 strains were Gordon’s genus Microbacterium,2 strains were Mycobacterium,and there was 1 Achromobacter,Micromonospora,Cellulomonas,and Agromyces respectively. It is suggested that there are rich actinomycetes with antifungal activity on the guts of Periplaneta americana.

Key words: Periplaneta americana, actinomycetes, antifungal activity, identification