外源褪黑素对干旱胁迫下牡丹幼苗生理特性的影响

doi:10.13560/j.cnki.biotech.bull.1985.2020-0055

摘要/Abstract

摘要： 为探究褪黑素（Melatonine,MT）对牡丹幼苗抗旱性的影响及生理机制,以牡丹品种凤丹（Paeonia ostii Fengdan）幼苗为试材,研究不同浓度外源褪黑素（50、100和200 μmol/L）对干旱胁迫下牡丹幼苗生长及生理特性的影响。结果表明,干旱胁迫严重限制了牡丹幼苗的生长和光合作用,叶片细胞脂质过氧化及伤害指数增加,引起氧化应激及渗透调节反应。经外源褪黑素预处理能够减轻干旱胁迫对牡丹幼苗的伤害,具体表现为根系、茎叶及总干物质量提高;减少叶绿素a、叶绿素b及类胡萝卜素等光合色素降解,改善光合作用及PSⅡ电子传输能力;降低丙二醛（MDA）、过氧化氢（H₂O₂）、超氧阴离子（O₂^-）含量及细胞膜质伤害指数（MII）,提高超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）及谷胱甘肽还原酶（GR）等抗氧化酶活性减少脂质过氧化;促进渗透调节物质积累。总之,以100 μmol/L MT处理对牡丹幼苗抗旱性提高效果最好。外源褪黑素预处理能提高干旱胁迫下牡丹幼苗光合荧光特性、抗氧化和渗透调节能力,促进干旱胁迫下牡丹幼苗生长。

关键词: 褪黑素, 干旱胁迫, 牡丹幼苗, 光合荧光特性, 抗氧化, 渗透调节

Abstract: To further explore the effects of exogenous melatonine（MT）in strengthening the ability of peony seedlings resisting drought and its physiological mechanisms,Paeonia ostii（Fengdan）seedlings were selected to study the effects of different concentrations（50,100,and 200 μmol/L）of exogenous MT on their growth and physiological characteristics under drought stress. The results showed that the growth and photosynthesis of the peony seedlings were significantly inhibited under drought stress,and lipid peroxidation and injury index of leaf cells increased,leading to oxidative stress and osmotic response. The damages caused by drought stress on the peony seedlings treated with the exogenous MT were alleviated,and specifically demonstrated as the mass of roots,stems,leaves and total dry matter increased,the degradation of photosynthetic pigments such as chlorophyll a,chlorophyll b and carotenoids reduced,and photosynthesis and PSⅡelectron transport ability improved,MDA,H₂O₂,O₂^- content and cell membrane damage index reduced,the activity of antioxidant enzymes such as SOD,CAT,APX and GR increased to reduce lipid peroxidation,and thus the accumulation of osmotic adjustment substances was improved. In general,100 μmol/L MT is considered to be the optimal concentration on improving the drought resistance of the peony seedlings. Pretreatment with exogenous MT may effectively enhance the photosynthetic fluorescence characteristics,antioxidant capacity and osmotic adjustment ability,thus promote the growth of peony seedlings under drought stress.

Key words: melatonin, drought stress, peony seedling, photosynthetic fluorescence characteristics, anti-oxidation, osmotic adjustment

范海霞, 赵飒, 辛国奇, 李静. 外源褪黑素对干旱胁迫下牡丹幼苗生理特性的影响[J]. 生物技术通报, 2020, 36(6): 63-72.

FAN Hai-xia, ZHAO Sa, XIN Guo-qi, LI Jing. Effects of Exogenous Melatonine on the Physiological Characteristics of Peony Seedlings Under Drought Stress[J]. Biotechnology Bulletin, 2020, 36(6): 63-72.

参考文献

[1] Chaves MM, João PM, João SP.Understanding plant responses to drought-from genes to the whole plant[J]. Functional Plant Biology, 2003, 30(3):239-264.
[2] Swann ALS.Plants and drought in a changing climate[J]. Current Climate Change Reports, 2018(4):192-201.
[3] 张腾国, 李巧丽, 刁志宏, 等. 盐及干旱胁迫对油菜抗氧化系统和基因表达的影响[J]. 应用生态学报, 2019, 30(3):969-978.
[4] 裴斌, 张光灿, 张淑勇, 等. 土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响[J]. 生态学报, 2013, 33(5):1386-1396.
[5] Zhang SH, Xu XF, Sun YM, et al.Influence of drought hardening on the resistance physiology of potato seedlings under drought stress[J]. Journal of Integrative Agriculture, 2018, 17(2):336-347.
[6] Mashilo J, Odindo AO, Shimelis HA, et al.Photosynthetic response of bottle gourd[Lagenaria siceraria, (Molina)Standl]to drought stress:Relationship between cucurbitacins accumulation and drought tolerance[J]. Scientia Horticulturae, 2018, 231:133-143.
[7] 王颖, 孔德浩, 陈佰鸿, 等. 外源ALA对干旱胁迫下山定子叶片叶绿素荧光特性及抗性生理指标的影响[J]. 西北植物学报, 2018, 38(5):902-911.
[8] 王书宏, 杜永吉. 外源激素对干旱胁迫下草莓光合特性的影响[J]. 中国农学通报, 2008(12):377-381.
[9] 叶君, 邓西平, 王仕稳, 等. 干旱胁迫下褪黑素对小麦幼苗生长、光合和抗氧化特性的影响[J]. 麦类作物学报, 2015(9):97-105.
[10] 杨小龙, 须晖, 李天来, 等. 外源褪黑素对干旱胁迫下番茄叶片光合作用的影响[J]. 中国农业科学, 2017, 50(16):3186-3195.
[11] Zhang HM, Zhang Y.Melatonin:a well-documented antioxidant with conditional pro-oxidant actions[J]. Journal of Pineal Research, 2014, 57(2):131-146.
[12] Janas KM, Małgorzata MP.Melatonin, an underestimated natural substance with great potential for agricultural application[J]. Acta Physiologiae Plantarum, 2013, 35(12):3285-3292.
[13] 邹京南, 曹亮, 王梦雪, 等. 外源褪黑素对干旱胁迫下大豆结荚期光合及生理的影响[J]. 生态学杂志, 2019, 38(9):2709-2718.
[14] Reiter R, Tan DX, Zhou Z, et al.Phytomelatonin:Assisting plants to survive and thrive[J]. Molecules, 2015, 20(4):7396-7437.
[15] Khana MN, Zhang J, Luo T.Seed priming with melatonin coping drought stress in rapeseed by regulating reactive oxygen species detoxification:Antioxidant defense system, osmotic adjustment, stomatal traits and chloroplast ultrastructure perseveration[J]. Industrial Crops & Products, 2019(140):1-11.
[16] Dong L, Yanqiu S, Zhiyou N, et al.Exogenous melatonin application delays senescence of kiwifruit leaves by regulating the antioxidant capacity and biosynthesis of flavonoids[J]. Frontiers in Plant Science, 2018(9):426-440.
[17] 史中飞, 梁娟红, 张小花, 等. 外源褪黑素对低温胁迫下油菜幼苗抗寒性的影响[J]. 干旱地区农业研究, 2019, 37(4):163-170.
[18] Wang LY, Liu JL, Wang WX, et al.Exogenous melatonin improves growth and photosynthetic capacity of cucumber under salinity-induced stress[J]. Photosynthetica, 2016, 54(1):19-27.
[19] Armagan K, Zeynep BD.Melatonin improves the multiple stress tolerance in pepper(Capsicum annuum)[J]. Scientia Horticulturae, 2019(256):1-9.
[20] 杨新元. 外源褪黑素对干旱胁迫下向日葵幼苗生长、光合及抗氧化系统的影响[J]. 华北农学报, 2019, 34(4):113-121.
[21] Cui GB, Zhao XX, Liu SD.Beneficial effects of melatonin in over-coming drought stress in wheat seedlings[J]. Plant Physiology and Biochemistry, 2017(118):138-149.
[22] 刘领, 李冬, 马宜林, 等. 外源褪黑素对干旱胁迫下烤烟幼苗生长的缓解效应与生理机制研究[J]. 草业学报, 2019(8):95-105.
[23] 贾清华, 刘爱青, 刘燕. 不同栽培方式下芍药生长开花及生物量变化[J]. 西南农业学报, 2013(2):354-358.
[24] 王琪, 刘建鑫, 张建军, 等. 水分胁迫对芍药生长和生理生化特性影响的研究[J]. 植物遗传资源学报, 2014(6):1270-1277.
[25] 王学奎, 黄建良. 植物生理生化实验原理与技术[M]. 北京:高等教育出版社, 2015.
[26] 李合生, 孙群, 赵世杰. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社, 2000.
[27] 林植芳, 李双顺, 林桂珠, 等. 衰老叶片和叶绿体中H₂O₂的累积与膜脂过氧化的关系[J]. 植物生理学报, 1988(1):19-25.
[28] 杨璐, 赵天宏. UV-B辐射增强对大豆根系活性氧代谢及抗氧化系统的影响[J]. 华北农学报, 2018, 33(5):178-184.
[29] Nayyar H.Accumulation of osmolytes and osmotic adjustment in water-stressed wheat(Triticum aestivum)and maize(Zea mays)as affected by calcium and its antagonists[J]. Environmental & Experimental Botany, 2003, 50(3):253-264.
[30] Constán-Aguilar C, Leyva R, Blasco B, et al.Biofortification with potassium:Antioxidant responses during postharvest of cherry tomato fruits in cold storage[J]. Acta Physiologiae Plantarum, 2014, 36(2):1-13.
[31] Muhammad ASR, Muhammad FS, Muhammad YA, et al.Glycinebetaine applied under drought improved the physiological efficiency of wheat(Triticum aestivum L.)plant[J]. Soil & Environment, 2012, 31(1):67-71.
[32] 厉恩茂, 李敏, 安秀红, 等. 叶面喷施褪黑素对干旱胁迫下苹果抗旱生理生化指标的影响[J]. 中国南方果树, 2019(4):95-98.
[33] Sarropoulou VN, Therios IN, Dimassi T, et al.Melatonin promotes adventitious root regeneration in in vitro shoot tip explants of the commercial sweet cherry rootstocks CAB-6P[J]. Journal of Pineal Research, 2012, 52(1):38-46.
[34] 吴燕, 连洪燕, 牟雪姣, 等. 干旱胁迫下叶面喷施褪黑素对滁菊幼苗生理生化特性的影响[J]. 西北植物学报, 2016(11):2241-2246.
[35] 梁新华, 许兴, 徐兆桢, 等. 干旱对春小麦旗叶叶绿素a荧光动力学特征及产量间关系的影响[J]. 干旱地区农业研究, 2001(3):74-79.
[36] Movahed N, Eshghi S, Jamali B, et al.Ameliorative effects of paclobutrazol on vegetative and physiological traits of grapevine cuttings under water stress condition[J]. Acta Horticulturae, 2012(931):475-483.
[37] Xiao X, Xu X, Yang F.Adaptive responses to progressive drought stress in two populus cathayana populations[J]. Silva Fennica, 2008, 42(5):705-719.
[38] 郑雨, 赵玉文, 费文群, 等. 干旱胁迫下西藏2种牡丹幼苗叶绿素含量和光合特征比较[J]. 高原农业, 2018, 2(5):3-11, 107.
[39] Liu JL, Wei X.Exogenous melatonin improves seedling health index and drought tolerance in tomato[J]. Plant Growth Regulation, 2015(77):317-326.
[40] Li H, Chang JJ, Chen HJ, et al.Exogenous melatonin confers salt stress tolerance to watermelon by improving photosynthesis and redox homeostasis[J]. Frontiers in Plant Science, 2017(8):295-298.
[41] 张环纬, 陈彪, 温心怡, 等. 外源硅对干旱胁迫下烟草幼苗生长、叶片光合及生理指标的影响[J]. 生物技术通报, 2019, 35(1):23-32.
[42] Kocheva K, Lambrev P, Georgiev G, et al.Evaluation of chlorophyll fluorescence and membrane injury in the leaves of barley cultivars under osmotic stress[J]. Bioelectrochemistry, 2004, 63(1/2):121-124.
[43] Wang P, Sun X, Li C, et al.Long-term exogenous application of melatonin delays drought-induced leaf senescence in apple[J]. Journal of Pineal Research, 2013, 54(3):292-302.
[44] Liting W, Lina W, Yang Y, et al.Abscisic acid enhances tolerance of wheat seedlings to drought and regulates transcript levels of genes encoding ascorbate-glutathione biosynthesis[J]. Frontiers in Plant Science, 2015(6):458-467.
[45] Leopold AC, Sun WQ, Bernal-Lugo I.The glassy state in seeds:analysis and function[J]. Seed Science Research, 1994, 4(3):267-274.