干细胞技术在地方猪种质资源保护中的应用前景

doi:10.13560/j.cnki.biotech.bull.1985.2020-0014

摘要/Abstract

摘要： 我国是养猪大国,拥有全球1/3猪种质资源。但近年来,我国地方品种猪养殖量急剧减少,部分品种几近灭绝,地方猪种遗传资源多样性遭受严重破坏,优质基因不断流失。如何有效保护和保存这些优良种质资源是非常紧迫和关键的问题。目前猪种质资源保护包括原位保存、精液和胚胎冷冻保存、DNA文库保种和体细胞冷冻保存等,但都存在一定局限性。干细胞是一类可以自我更新并具有分化潜能的细胞群体,它不仅能分化成不同类型的细胞,还可以通过分裂维持自身群体稳定。在小鼠和猪等动物上,利用干细胞已成功获得健康后代。最近,以干细胞研究为引领的种质资源保护新浪潮正在兴起,干细胞技术将成为我国地方猪种质资源保护的新手段。

关键词: 干细胞技术, 地方猪, 种质资源, 保护

Abstract: Our country is one where a large amount of pigs is breeding,owing 1/3 of the global porcine germplasm resources. However,in recent years,the breeding volume of indigenous pig has decreased sharply,and some breeds are almost extinct. The genetic resource diversity of indigenous pig breeds in China has been seriously damaged,and high-quality genes have been lost continuously. How to effectively protect and conserve these excellent germplasm resources is an urgent and critical issue. At present,the protection of porcine germplasm resources includes in situ conservation,cryopreservation of semen and embryos,DNA library conservation and cryopreservation of somatic cells;however on which there are some limitations. Stem cells are a type of cell population that can self-renew and have the potential to differentiate. It can not only differentiate into different types of cells,but also maintain the stability of its own population through division. Healthy offspring have been created successfully by stem cells in pigs and mice. Recently,a new wave of germplasm resource conservation led by stem cell research is emerging. Stem cell technology may become a new means to protect indigenous porcine germplasm resources.

Key words: stem cell technology, native pig breeds, germplasm resources, conservation

孙嘉栋, 孙晓凤, 李兰, 沈伟, 程顺峰. 干细胞技术在地方猪种质资源保护中的应用前景[J]. 生物技术通报, 2020, 36(8): 228-234.

SUN Jia-dong, SUN Xiao-feng, LI Lan, SHEN Wei, CHENG Shun-feng. Application Prospects of Stem Cell Technology in the Protection of Indigenous Porcine Germplasm Resources[J]. Biotechnology Bulletin, 2020, 36(8): 228-234.

参考文献

[1] 张仲葛, 李炳坦, 陈效华. 中国猪品种志[M]. 上海:上海科学技术出版社, 1986.
Zhang ZG, Li BT, Chen XH.Pig breeds in China[M]. Shanghai:Shanghai Science & Technology Publishers, 1986.
[2] 联合国粮食与农业组织. 世界粮食与农业动物遗传资源状况[M]. 北京:中国农业出版社, 2007.
Food and Agriculture Organization of the United Nations. The genetic resources state of the world's food and agriculture animals[M]. Beijing:China Agriculture Press, 2007.
[3] 陈其美, 葛长利, 董彬. 中国地方猪种种质资源的保护与利用[J]. 中国猪业, 2013, 8(S2):160-163.
Chen QM, Ge CL, Dong B.Protection and utilization of local pig germplasm resources in China[J]. China Swine Industry, 2013, 8(S2):160-163.
[4] 王辉田. 中国地方猪种遗传资源保护策略研究[J]. 猪业科学, 2014, 31(3):131-132.
Wang HT.Research on genetic resources conservation strategies of local pig breeds in China[J]. China Swine Industry, 2014, 31(3):131-132.
[5] 路国彬, 王夏晖, 吕文魁, 等. 中国畜禽遗传资源保护问题分析[J]. 家畜生态学报, 2014, 35(4):1-6.
Lu GB, Wang XH, Lv WK, et al.Analysis of conservation status of Chinese livestock and poultry genetic resources[J]. Journal of Domestic Animal Ecology, 2014, 35(4):1-6.
[6] 宫时玉, 蒋曹德, 邓昌彦. 我国地方猪品种资源遗传多样性研究进展[J]. 湖北农业科学, 2004(5):79-82.
Gong SY, Jiang CD, Deng CY.Research progress on genetic diversity of local pig variety resources in China[J]. Hubei Agricultural Sciences, 2004(5):79-82.
[7] 关伟军, 马月辉. 频危畜禽品种细胞库的构建与鉴定[J]. 中国农业科技导报, 2003(5):66-70.
Guan WJ, Ma YH.Construction and identification of cell bank of Frequently endangered animal and poultry species[J]. Journal of Agricultural Science and Technology, 2003(5):66-70.
[8] 郭瑜. 牛成纤维细胞库的建立及生物学特性研究[D]. 雅安:四川农业大学, 2005.
Guo Y.Constructing and biological study of cattle fibroblast cell lines[D]. Yaan:Sichuan Agricultural University, 2005.
[9] 周雪雁. 七个羊品种成纤维细胞库构建及其生物学特性研究[D]. 太谷:山西农业大学, 2005.
Zhou XY.Establishment and biological characteristics of fibroblast cell bank in seven goat and sheep breeds[D]. Taigu:Shanxi Agricultural University, 2005.
[10] 跃华. 大骨鸡、青壳蛋鸡成纤维细胞库的建立及生物学特性研究[D]. 呼和浩特:内蒙古农业大学, 2006.
Yue H.Research on the establishment and characterizing of DaGu chicken and QingKe chicken fibroblast cell lines[D]. Hohhot:Inner Mongolia Agricultural University, 2006.
[11] 刘牧, 张金国, 刘维俊, 等. 中国建立“冷冻动物园”浅析[J]. 野生动物, 2011, 32(1):41-45.
Liu M, Zhang JG, Liu WJ, et al.Conservation of wild animal resources and the“frozen zoo”[J]. Chinese Journal of Wildlife, 2011, 32(1):41-45.
[12] 李凌松, 朱建健, 杜仪琴, 等. 干细胞的研究进展及临床应用前景[J]. 生理科学进展, 2001(2):138-140.
Li LS, Zhu JJ, Du YQ, et al.Research progress and clinical application prospect of stem cells[J]. Progress in Physiological Sciences, 2001(2):138-140.
[13] Fujishiro S, Nakano K, Mizukami Y, et al.Generation of naive-like porcine-induced pluripotent stem cells capable of contributing to embryonic and fetal development[J]. Stem Cells and Development, 2013, 22(3):473-482.
[14] 王寒凝. 猪诱导多能干细胞向雄性生殖细胞样细胞诱导分化的研究[D]. 北京:中国农业大学, 2017.
Wang HN.Derivation of male germ cell-like cells from porcine induced pluripotent stem cells[D]. Beijing:China Agricultural University, 2017.
[15] 杨志平. 中国地方猪种资源的保护和利用[J]. 中国畜牧兽医文摘, 2017, 33(12):73.
Yang ZP.Protection and utilization of local pig resources in China[J]. Abstract of Animal Husbandry and Veterinary of China, 2017, 33(12):73.
[16] 王志刚. 我国畜禽遗传资源保护手段分析[J]. 中国牧业通讯, 2005(13):4-7.
Wang ZG.Analysis on the protection means of livestock and poultry genetic resources in China[J]. China Animal Industry, 2005(13):4-7.
[17] 陈岩锋, 谢喜平. 畜禽遗传资源保存理论与方法研究进展[J]. 福建畜牧兽医, 2007(4):58-60.
Chen YF, Xie XP.Research progress of conservation theory and method of livestock and poultry genetic resources[J]. Fujian Journal of Animal Husbandry and Veterinary Medicine, 2007(4):58-60.
[18] 刘丑生, 刘刚, 陆健, 等. 我国国家畜禽基因库的现状和前景[J]. 中国畜牧杂志, 2014, 50(12):10-16.
Liu CS, Liu G, Lu J, et al.Current situation and prospect of national livestock and poultry gene bank in China[J]. Chinese Journal of Animal Science, 2014, 50(12):10-16.
[19] 吴梦, 刘雪芹, 刘子嘉, 等. 猪精液超低温冷冻保存研究进展[J]. 中国畜牧杂志, 2019, 55(7):35-40.
Wu M, Liu XQ, Liu ZJ, et al.Research progress on cryopreservation of boar semen[J]. Chinese Journal of Animal Science, 2019, 55(7):35-40.
[20] 杨山亭. 猪胚胎玻璃化冷冻及胚胎生产技术的研究[D]. 南京:南京农业大学, 2009.
Yang ST.Research on vitrification and freezing of pig embryo and embryo production technology[D]. Nanjing:Nanjing Agricultu-ral University, 2009.
[21] Holt WV, Pickard AR, et al.Wildlife conservation and reproductive cloning[J]. Reproduction, 2004, 127(3):317-324.
[22] Liu Y, Li J, Løvendahl P, et al.In vitro manipulation techniques of porcine embryos:a meta-analysis related to transfers, pregnancies and piglets[J]. Reproduction, 2015, 27(3):429-439.
[23] Schmidt M, Winther K, Secher J, et al.Postmortem findings in cloned and transgenic piglets dead before weaning[J]. Theriogenology, 2015, 84(6):1014-1023.
[24] 王珊珊. 干细胞的研究历程及其临床应用研究展望[J]. 农业灾害研究, 2015, 5(12):20-21.
Wang SS.Research course of stem cells and research prospect of its clinical application[J]. Journal of Agricultural Catastrophology, 2015, 5(12):20-21.
[25] Evans M, et al.Establishment in culture of pluripotential cells from mouse embryos[J]. Nature, 1981, 292(5819):154-156.
[26] 田胜男, 王博, 李琦, 等. 诱导多能干细胞的安全性及应用研究进展[J]. 中国组织工程研究, 2017, 21(5):815-820.
Tian SN, Wang B, Li Q, et al.The safety and application of induced pluripotent stem cells[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(5):815-820.
[27] Takahashi K, Yamanaka S.Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors[J]. Cell, 2006, 126(4):663-676.
[28] Zhao XY, et al.iPS cells produce viable mice through tetraploid complementation[J]. Nature, 2009, 461(7260):86-90.
[29] Kang L, Wang JL, Zhang Y, et al.iPS cells can support full-term development of tetraploid blastocyst-complemented embryos[J]. Cell Stem Cell, 2009, 5(2):135-138.
[30] Sun YC, Cheng SF, Sun R, et al.Reconstitution of gametogenesis in vitro:meiosis is the biggest obstacle[J]. Journal of Genetics and Genomics, 2014, 41(3):87-95.
[31] Blanpain C, Lowry WE, Geoghegan A, et al.Self-renewal, multipotency, and the existence of two cell populations within an epithelial stem cell niche[J]. Cell, 2004, 118(5):635-648.
[32] 赵颖, 孔庆然, 刘忠华. 猪胚胎干细胞研究进展[J]. 中国细胞生物学学报, 2010, 32(5):815-821.
Zhao Y, Kong QR, Liu ZH.Advances in research on pig embryonic stem cells[J]. Chinese Journal of Cell Biology, 2010, 32(5):815-821.
[33] Ramaswamy K, Yik WY, Wang XM, et al.Derivation of induced pluripotent stem cells from orangutan skin fibroblasts[J]. BMC Research Notes, 2015, 8(1):577-585.
[34] Honda A, Choijookhuu N, Izu H, et al.Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis[J]. Science Advances, 2017, 3(5):e1602179-e1602187.
[35] Stanton MM, et al.Prospects for the use of induced pluripotent stem cells in animal conservation and environmental protection[J]. Stem Cells Transl Med, 2019, 8(1):7-13.
[36] Selvaraj V, Wildt DE, Pukazhenthi BS.Induced pluripotent stem cells for conserving endangered species?[J]. Nature Methods, 2011, 8(10):805-807.
[37] Hayashi K, Ogushi S, Kurimoto K, et al.Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice[J]. Science, 2012, 338(6109):971-975.
[38] Hikabe O, Hamazaki N, Nagamatsu G, et al.Reconstitution in vitro of the entire cycle of the mouse female germ line[J]. Nature, 2016, 539(7628):299-303.
[39] Evans M, Notarianni E, Laurie S, et al.Derivation and preliminary characterization of pluripotent cell lines from porcine and bovine blastocysts[J]. Theriogenology, 1990, 33(1):125-128.
[40] Xue BH, Li Y, He YL, et al.Porcine pluripotent stem cells derived from IVF embryos contribute to chimeric development in vivo[J]. PLoS One, 2016, 11(3):e0151737-e0151753.
[41] Zhang X, Xue BH, Li Y, et al.A novel chemically defined serum- and feeder-free medium for undifferentiated growth of porcine pluripotent stem cells[J]. Journal of Cellular Physiology, 2019, 234(9):15380-15394.
[42] 薛冰华. 胚胎来源的猪多能性干细胞系建立及其多能性改善研究[D]. 哈尔滨:东北农业大学, 2016.
Xue BH.The establishment and pluripotency improvement of porcine pluripotent stem cells derived from porcine embryos[D]. Harbin:Northeast Agricultural University, 2016.
[43] Boland MJ, Hazen JL, et al.Adult mice generated from induced pluripotent stem cells[J]. Nature, 2009, 461(7260):91-94.
[44] 吴翠玲, 张玉明. 诱导性多能干细胞技术的研究进展及其应用前景[J]. 中国实验血液学杂志, 2014, 22(4):883-888.
Wu CL, Zhang YM.Progress and potential applications of induced pluripotent stem cell technology[J]. Journal of Experimental Hematology, 2014, 22(4):883-888.
[45] Ezashi T, Telugu BPV, Alexenko AP, et al.Derivation of induced pluripotent stem cells from porcine somatic cells[J]. PNAS, 2009, 106(27):10993-10998.
[46] West FD, Terlouw SL, Kwon DJ, et al.Porcine induced pluripotent stem cells produce chimeric offspring[J]. Stem Cells and Development, 2010, 19(8):1211-1220.
[47] Fan NN, Chen JJ, et al.Piglets cloned from induced pluripotent stem cells[J]. Cell Research, 2013, 23(1):162-166.
[48] Oback B.Cloning from stem cells:different lineages, different species, same story[J]. Reproduction, 2009, 21(1):83-94.
[49] 寇朝辉. 利用核移植技术从iPS细胞获得克隆小鼠[D]. 哈尔滨:东北农业大学, 2012.
Kou CH.Mice cloned from induced pluripotent stem cells[D]. Harbin:Northeast Agricultural University, 2012.
[50] Secher JO, Liu Y, et al.Evaluation of porcine stem cell competence for somatic cell nuclear transfer and production of cloned animals[J]. Animal Reproduction Science, 2017, 178:40-49.
[51] Bhartiya D, Anand S, Patel H, et al.Making gametes from alternate sources of stem cells:past, present and future[J]. Reproductive Biology and Endocrinology, 2017, 15(1):89-102.
[52] Toma J, Akhavan M, Fernandes K, et al.Isolation of multipotent adult stem cells from the dermis of mammalian skin[J]. Nature Cell Biology, 2001, 3(9):778-784.
[53] Dyce PW, Zhu H, Craig J, et al.Stem cells with multilineage potential derived from porcine skin[J]. Biochemical and Biophysical Research Communications, 2004, 316(3):651-658.
[54] Dyce PW, Li JL.From skin cells to ovarian follicles?[J]. Cell Cycle, 2006, 5(13):1371-1375.
[55] Dyce PW, Shen W, Huynh E, et al.Analysis of oocyte-like cells differentiated from porcine fetal skin-derived stem cells[J]. Stem Cells and Development, 2011, 20(5):809-819.
[56] Song SH, et al.Characterization of porcine multipotent stem/stromal cells derived from skin, adipose, and ovarian tissues and their differentiation in vitro into putative oocyte-like cells[J]. Stem Cells and Development, 2011, 20(8):1359-1370.
[57] 陈庭锋. 猪精原干细胞分离培养鉴定及诱导分化的研究[D]. 扬州:扬州大学, 2014.
Chen TF.Study on culture and differentiation of pig spermatogonia-lstem cells in vitro[D]. Yangzhou:Yangzhou University, 2014.
[58] Sato T, Katagiri K, Yokonishi T, et al.In vitro production of fertile sperm from murine spermatogonial stem cell lines[J]. Nature Communications, 2011, 2(1):472-478.
[59] Zhao HM, Nie JY, et al.In vitro differentiation of spermatogonial stem cells using testicular cells from Guangxi Bama mini-pig[J]. Journal of Veterinary Science, 2018, 19(5):592-599.

编辑推荐 0

Metrics

阅读次数

全文

550

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	0	0	0	550

来源	本网站	其他网站

次数	499	51
比例	91%	9%

摘要

369

最新录用	在线预览	正式出版

0	0	369

	来源	本网站

	次数	369
	比例	100%