生物技术通报 ›› 2018, Vol. 34 ›› Issue (6): 115-119.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0054

• 研究报告 • 上一篇    下一篇

木瓜秀粉蚧取食不同木薯品种后体内保护酶活性差异分析

王亚茹1,2 ,梁晓2 ,伍春玲2 ,陈青2 ,赵惠萍2   

  1. 1. 华中农业大学植物科学技术学院,武汉 430070;
    2. 中国热带农业科学院环境与植物保护研究所 农业部热带作物有害生物综合治理重点实验室 海南省热带作物病虫害生物防治工程技术研究中心 热带农业有害生物监测与控制重点实验室,海口 571101
  • 收稿日期:2018-01-17 出版日期:2018-06-26 发布日期:2018-07-03
  • 作者简介:王亚茹,女,硕士研究生,研究方向:农业昆虫与害虫防治;E-mail:chestnutzzz@163.com
  • 基金资助:
    国家木薯产业技术体系虫害防控岗位科学家专项(CARS-11-HNCQ),中央级公益性科研院所基本科研业务费专项(2017hzs1J013,2017hzs1J019),农业部统计监测与信息服务项目(20180308)

Activity Variations of Protective Enzymes in Paracoccus marginatus After Fed Different Cassava Cultivars

WANG Ya-ru1,2 ,LIANG Xiao2 ,WU Chun-ling2 ,CHEN Qing2 ,ZHAO Hui-ping2   

  1. 1. College of Plant Science & Technology,Huazhong Agricultural University,Wuhan 430070;
    2. Environment and Plant Protection Institute,China Academy of Tropical Agricultural Sciences/Key Laboratory of Pests Comprehensive Governance for Tropical Crops,Ministry of Agriculture/Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests/Key Laboratory for Monitoring and Control of Tropical Agricultural Pests,Haikou 571101
  • Received:2018-01-17 Published:2018-06-26 Online:2018-07-03

摘要: 为深入探讨木瓜秀粉蚧的寄主选择性机理,采用紫外分光光度法测定分析了取食不同木薯品种叶片48 h后,木瓜秀粉蚧体内过氧化物酶(POD)、多酚氧化酶(PPO)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性的差异。结果表明,取食木薯品种C1115、瑞士F21和缅甸种的木瓜秀粉蚧雌成虫体内POD、PPO、SOD和CAT的活性较取食对照品种BRA900的木瓜秀粉蚧相同保护酶活性显著降低(P﹤0.05),仅分别为对照的42.2%-44.0%,62.3%-63.5%,35.9%-52.9%和50.3%-55.7%,而取食木薯品种SC205和ZM9066的保护酶活性与对照相比无显著差异。上述结果说明,木薯品种C1115、瑞士F21和缅甸种对木瓜秀粉蚧雌成虫POD、PPO、SOD和CAT表现出较强的抑制作用,而BRA900、SC205和ZM9066对木瓜秀粉蚧保护酶活性无显著抑制作用,易于被取食为害。

关键词: 木瓜秀粉蚧, 木薯, 保护酶, 活性变化

Abstract: To explore the mechanism of Paracoccus marginatus selecting host plants,the activity variations of peroxidase(POD),polyphenol oxidase(PPO),catalase(CAT)and superoxide dismutase(SOD)in P. marginatus after fed different cassava cultivars for 48 h were determined by ultraviolet spectrophotometry. The results showed that the enzymes’ activities in adult females fed with cultivar C1115,Swiss F21 and Myanmar were significantly lower than those fed with the control cultivar BRA900(P ﹤0.05),the decreasing range were listed as:41%-43%(POD),60%-63%(PPO),35%-55%(SOD)and 54%-60%(CAT),while those in adult females fed with cultivar SC205 and ZM9066 did not show significant difference. Above results indicate that cassava cultivar C1115,F21 and Myanmar show strong inhibitory effects on the activities of POD,PPO,SOD and CAT in P. marginatus,while BRA900,SC205 and ZM9066 do not show significant inhibitory effects on those protective enzymes,which inclines them to be damaged by P. marginatus.

Key words: Paracoccus marginatus, cassava cultivar, protective enzymes, activity variation