猪流行性腹泻病毒蛋白拮抗宿主天然免疫应答的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2020-0436

摘要/Abstract

摘要：

猪流行性腹泻病毒（Porcine epidemic diarrhea virus,PEDV）是引起猪腹泻的主要病原之一,可导致猪呕吐、水样腹泻和严重脱水等症状,对仔猪的致死率高达100%,给养猪业造成了巨大的经济损失。PEDV编码的21个蛋白不仅参与病毒增殖,而且在拮抗宿主天然免疫应答中发挥重要作用。目前,已有10个蛋白被证实具有限制或阻断干扰素应答的能力,其通过不同的机制逃避宿主的免疫应答。为了深入理解这些蛋白在病毒感染中的作用以及调控宿主免疫反应的机制,本文对调控宿主免疫应答的PEDV蛋白进行总结,以期为PEDV疫苗的创制和疾病防控奠定理论基础。

关键词: 猪流行性腹泻病毒, 天然免疫, 病毒蛋白, 免疫抑制

Abstract:

Porcine epidemic diarrhea virus is one of major causative agents of diarrhea in pigs that causes vomiting,watery diarrhea,and severe dehydration in swine,and with topped 100% mortality in neonatal piglets,leading to enormous economic losses within the swine industry worldwide. The encoded 21 proteins of PEDV are not only involved in virus life cycle,but also play critical role in antagonizing host innate immune responses. Presently,ten viral proteins have been confirmed to have abilities in inhibition interferon responses,i.e.,host immune system was escaped through different mechanisms. In order to deeply understand the functions of these proteins in viral infection and mechanisms in regulation host immune responses,the recent progress on the PEDV proteins involved in host immune regulation has been reviewed,aiming to provide theoretical basis for PEDV vaccine development and disease control.

Key words: porcine epidemic diarrhea virus, innate immune, viral proteins, immunosuppression

成温玉, 白云, 贾怀杰, 强桃艳, 赵鸿远, 张博艺, 郭晓荟. 猪流行性腹泻病毒蛋白拮抗宿主天然免疫应答的研究进展[J]. 生物技术通报, 2020, 36(12): 229-238.

CHENG Wen-yu, BAI Yun, JIA Huai-jie, QIANG Tao-yan, ZHAO Hong-yuan, ZHANG Bo-yi, GUO Xiao-hui. Research Progress on Proteins of PEDV Antagonizing Host Innate Immune Responses[J]. Biotechnology Bulletin, 2020, 36(12): 229-238.

图/表 1

图1 PEDV蛋白拮抗宿主天然免疫信号通路氨肽酶N（Aminopeptidase-N,APN）;CREB结合蛋白（CREB-binding protein,CBP）;黑色素瘤分化相关蛋白5（Melanoma differentiation-associated protein 5,MDA5）;维甲酸诱导基因Ⅰ（Retinoic acid inducible gene-I,RIG-I）;线粒体抗病毒信号蛋白（Mitochondrial antiviral signaling,MAVS）;干扰素刺激因子（Stimulator of interferon gene,STING）;肿瘤坏死困子受体相关因子（TNF receptor associated factor,TRAF）;含诱导IFN-β接头的TIR结构域（TIR-domain-containing adapter-inducing IFN-β,TRIF）;核转录因子关必须调节蛋白（NF-κB essential modulator,NEMO）;M丝裂原活化蛋白激酶（Mitogen activated protein kinases,MAPK）;髓样分化因子88（Myeloid differentiation factor 88,MyD88）;Toll样受体（Toll-like receptor,TLR）;TANK结合激酶1（TANK-binding kinase 1,TBK）;干扰素调节因子（IFN-regulatory factor,IRF）;干扰素刺激基因（Interferon stimulated gene,ISG）;信号传导与转录激活子（Signal transducers and activators of transcription,STAT）;Janus激酶（Janus kinase,JAK）。表示促进; 表示抑制; 表示剪切

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