真菌介导纳米银生物合成的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2020-0952

摘要/Abstract

摘要：

纳米银（Silver nanoparticles,AgNPs）是指三维结构中至少有一维在1-100 nm范围内的金属银材料,在光学、电学、催化和医药等领域得到了广泛应用。利用真菌生物合成AgNPs相比于传统的化学、物理合成方法,具有反应条件温和、绿色低毒、环境友好等优势备受关注。综述了近年来合成AgNPs真菌种类的研究进展,归纳了真菌合成AgNPs位点的优缺点;讨论影响真菌合成AgNPs尺寸、形貌及合成速率的因素;重点总结了合成机制的共识;介绍了AgNPs在生物医药、农业、催化剂等领域中的应用;最后指出真菌合成AgNPs还需深入研究的问题,对未来的可能突破进行展望,以期为今后真菌合成AgNPs的工业化生产提供参考与理论支撑。

关键词: 真菌, 纳米银, 生物合成, 影响因素, 机制

Abstract:

Silver nanoparticles（AgNPs）refer to metallic silver materials with at least one dimension in the range of 1-100 nm in the three-dimensional structure,and are used in the fields of electricity,optics,catalysis,mechanical materials and medicine. Compared with traditional chemical and physical synthesis methods,biosynthesis of AgNPs via fungi have attracted the attention of the scientific researchers because of its mild reaction conditions,green and low toxicity,environment-friendly,etc. Firstly,the recent research progress in fungi species for the synthesis of AgNPs is reviewed and the advantages and disadvantages of fungi synthesis of AgNPs sites are summed up. The factors affecting the size,shape and synthesis rate of fungi synthesized AgNPs are discussed. Secondly,the consensus of the synthesis mechanism is summarized. Furthermore,the application of AgNPs in biomedicine,agriculture,catalysts and other fields are introduced. Finally,the future opportunities and challenges on the synthesis of AgNPs by fungi are prospected. It is aimed to provide reference and theoretical support for the further industrial production of AgNPs synthesized by fungi.

Key words: fungi, sliver nanoparticles, biosynthesis, regulatory factors, mechanism

乔自鹏, 王奇志, 杨道茂, 阮丽萍. 真菌介导纳米银生物合成的研究进展[J]. 生物技术通报, 2021, 37(3): 185-197.

QIAO Zi-peng, WANG Qi-zhi, YANG Dao-mao, RUAN Li-ping. Research Progress in Fungi-mediated Biosynthesis of Sliver Nanoparticles[J]. Biotechnology Bulletin, 2021, 37(3): 185-197.

图/表 3

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真菌	形状	尺寸	合成位点	参考文献
粉节皮菌（Arthroderma fulvum）	球形	20.56	胞外	[21]
烟曲霉菌（Aspergillus fumigatus BTCB10）	球形	0.681	胞外	[22]
米曲霉（Aspergillus oryzae MTCC No.1846）	球形	7-27	胞外	[23]
土曲霉（Aspergillus terreus）	球形	45.2	胞外	[24]
可可毛色二孢菌（BotryospHaeria rhodian）	球形	2-50	胞外	[25]
芽枝状枝孢（Cladosporium cladosporioides）	-	30-60	胞外	[26]
Colletotrichum incarnatum DM16.3	球形	5-25	胞外	[27]
刺盘孢属（Colleotrichum sp. ALF2-6）	球形、近球形、三角形、六角形	20-50	胞外	[19]
嗜线虫真菌（Duddingtonia flagans）	球形	11.38	胞外	[28]
尖孢镰刀菌（Fusarium oxysporum）	球形	24	胞外	[29]
芒果球座菌（Guignardia mangiferae）	球形	5-30	胞外	[30]
玫烟色棒束孢（Isaria fumosorosea）	球形	51.31-111.02	胞外	[31]
色二孢（Lasiodiplodia theobromae）	球形	62.77103 66.75-111.23	胞内胞外	[32]
马利亚霉菌（Mariannaea sp. HJ）	球形、伪球形、棒状	15	胞内	[33]
产黄青霉（Penicillium chrysogenum FGCC/BLS1）	椭球形	96.8	胞外	[34]
柑橘青霉菌（Penicillium italicum）	球形	33	胞外	[35]
草酸青霉菌（Penicillium oxalicum）	球形	4	胞外	[36]
草酸青霉菌（Penicillium oxalicum GRS-1）	球形	10-40	胞内	[37]
波兰青霉（Penicillium polonicum ARA 10）	球形	10-15	胞外	[38]
枫香拟茎点霉（Phomopsis liquidambaris）	球形	18.7	胞外	[39]
平菇菌（Pleurotus ostreatus）	球形	< 40	-	[40]
匍枝根霉（Rhizopus stolonifera）	球形	2.86	胞外	[41]
链霉菌属（Streptomyces sp. VSMGT1014）	球形	-	胞外	[42]
Talaromyces purpureogenus	球形、三角形	< 50	胞内	[43]
深绿木霉（Trichoderma atroviride）	球形	10-15	胞外	[44]
钩状木霉（Trichoderma hamatum）	球形	6.69	胞外	[45]
哈茨木霉菌（Trichoderma harzianum）	-	57.02± 1.75-81.84± 0.67	胞外	[46]
长枝木霉（Trichoderma longibrachiatum）	球形	24.43	胞外	[47]
里氏木霉（Trichoderma reesei）	球形	15-25	胞外	[48]
绿色木霉菌（Trichoderma viride）	球形	3-16	胞外	[49]

真菌	形状	尺寸	合成位点	参考文献
粉节皮菌（Arthroderma fulvum）	球形	20.56	胞外	[21]
烟曲霉菌（Aspergillus fumigatus BTCB10）	球形	0.681	胞外	[22]
米曲霉（Aspergillus oryzae MTCC No.1846）	球形	7-27	胞外	[23]
土曲霉（Aspergillus terreus）	球形	45.2	胞外	[24]
可可毛色二孢菌（BotryospHaeria rhodian）	球形	2-50	胞外	[25]
芽枝状枝孢（Cladosporium cladosporioides）	-	30-60	胞外	[26]
Colletotrichum incarnatum DM16.3	球形	5-25	胞外	[27]
刺盘孢属（Colleotrichum sp. ALF2-6）	球形、近球形、三角形、六角形	20-50	胞外	[19]
嗜线虫真菌（Duddingtonia flagans）	球形	11.38	胞外	[28]
尖孢镰刀菌（Fusarium oxysporum）	球形	24	胞外	[29]
芒果球座菌（Guignardia mangiferae）	球形	5-30	胞外	[30]
玫烟色棒束孢（Isaria fumosorosea）	球形	51.31-111.02	胞外	[31]
色二孢（Lasiodiplodia theobromae）	球形	62.77103 66.75-111.23	胞内胞外	[32]
马利亚霉菌（Mariannaea sp. HJ）	球形、伪球形、棒状	15	胞内	[33]
产黄青霉（Penicillium chrysogenum FGCC/BLS1）	椭球形	96.8	胞外	[34]
柑橘青霉菌（Penicillium italicum）	球形	33	胞外	[35]
草酸青霉菌（Penicillium oxalicum）	球形	4	胞外	[36]
草酸青霉菌（Penicillium oxalicum GRS-1）	球形	10-40	胞内	[37]
波兰青霉（Penicillium polonicum ARA 10）	球形	10-15	胞外	[38]
枫香拟茎点霉（Phomopsis liquidambaris）	球形	18.7	胞外	[39]
平菇菌（Pleurotus ostreatus）	球形	< 40	-	[40]
匍枝根霉（Rhizopus stolonifera）	球形	2.86	胞外	[41]
链霉菌属（Streptomyces sp. VSMGT1014）	球形	-	胞外	[42]
Talaromyces purpureogenus	球形、三角形	< 50	胞内	[43]
深绿木霉（Trichoderma atroviride）	球形	10-15	胞外	[44]
钩状木霉（Trichoderma hamatum）	球形	6.69	胞外	[45]
哈茨木霉菌（Trichoderma harzianum）	-	57.02± 1.75-81.84± 0.67	胞外	[46]
长枝木霉（Trichoderma longibrachiatum）	球形	24.43	胞外	[47]
里氏木霉（Trichoderma reesei）	球形	15-25	胞外	[48]
绿色木霉菌（Trichoderma viride）	球形	3-16	胞外	[49]