生物技术通报 ›› 2021, Vol. 37 ›› Issue (4): 107-115.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1094
朱淑芳(), EHENEDEN Iyobosa, 宁海军, 尚洁昊, 李武阳, 孟宪刚()
收稿日期:
2020-08-29
出版日期:
2021-04-26
发布日期:
2021-05-13
作者简介:
朱淑芳,女,硕士研究生,研究方向:含油污泥的处置;E-mail:ZHU Shu-fang(), EHENEDEN Iyobosa, NING Hai-jun, SHANG Jie-hao, LI Wu-yang, MENG Xian-gang()
Received:
2020-08-29
Published:
2021-04-26
Online:
2021-05-13
摘要:
为获得高效原油降解菌株,从山东某油田的含油污泥中和实验室自有菌剂以及原油污染的土壤中分离筛选得到9株微生物,分别编号SF1、SF2、SF3、SF4、SF5、SF6、SF7、SF8和SF9。研究表明,9株菌对原油均有一定的降解能力。通过16S rRNA鉴定表明:SF1为中间苍白杆菌属(Ochrobactrum intermedium),SF2、SF8属于肠杆菌属(Enterobacter sp.),SF3、SF7为不动杆菌属(Acinetobacter sp.),SF4、SF5、SF6、SF9属于假单胞菌菌属(Pseudomonas sp.)。其中,菌株SF1降解能力最强,降解率达48.73%,菌株SF2、SF7、SF9的降解能力较强,降解率分别为41.90%、45.35%、32.25%,其他菌株的降解率也高于20%。通过正交实验得出,由菌株SF1、SF2、SF3、SF4、SF5、SF6、SF7和SF9且添加量为2∶1∶1∶1∶1∶1∶2∶2组成的菌群对原油的降解效果优于单个菌株,该菌群为最佳高效菌群,降解率达57.88%。
朱淑芳, EHENEDEN Iyobosa, 宁海军, 尚洁昊, 李武阳, 孟宪刚. 高效原油污染降解菌的筛选、鉴定及菌群的构建[J]. 生物技术通报, 2021, 37(4): 107-115.
ZHU Shu-fang, EHENEDEN Iyobosa, NING Hai-jun, SHANG Jie-hao, LI Wu-yang, MENG Xian-gang. Screening and Identification of Highly Efficient Crude Oil-degrading Bacteria and Construction of Bacterial Consortium[J]. Biotechnology Bulletin, 2021, 37(4): 107-115.
图2 DCPIP定性筛选原油降解菌株 A:培养24 h;B:培养48 h;C:培养72 h
Fig.2 Qualitative screening of crude oil-degrading strains by DCPIP A: Cultivate for 12 h. B: Cultivate for 48 h. C: Cultivate for 72 h
实验组Experimental group | SF1 | SF2 | SF3 | SF4 | SF5 | SF6 | SF7 | SF8 | SF9 | 降解率Degradation rate/% |
---|---|---|---|---|---|---|---|---|---|---|
1 | 0 | 0 | 1 | 0.5 | 0.5 | 0 | 0.5 | 0 | 0 | 30.20 |
2 | 1 | 0.5 | 1 | 0 | 0 | 0.5 | 0.5 | 0 | 0 | 38.00 |
3 | 0.5 | 0.5 | 1 | 0.5 | 0 | 0 | 1 | 0.5 | 0.5 | 41.25 |
4 | 0.5 | 0.5 | 0.5 | 1 | 0.5 | 0 | 0 | 0.5 | 0 | 43.60 |
5 | 1 | 0 | 0.5 | 0 | 1 | 1 | 0 | 0.5 | 1 | 37.10 |
6 | 0.5 | 1 | 0.5 | 0 | 0 | 1 | 1 | 0 | 0 | 31.1 |
7 | 0 | 1 | 0.5 | 0.5 | 0 | 0.5 | 0 | 0.5 | 0.5 | 34.45 |
8 | 0.5 | 0 | 0.5 | 0.5 | 1 | 0.5 | 0.5 | 1 | 0 | 28.60 |
9 | 1 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 1 | 0 | 1 | 57.88 |
10 | 1 | 1 | 1 | 0.5 | 1 | 0 | 0 | 1 | 0 | 24.90 |
11 | 0.5 | 0 | 0 | 1 | 0 | 0.5 | 1 | 1 | 1 | 28.55 |
12 | 0 | 0.5 | 0 | 0.5 | 1 | 1 | 1 | 1 | 0 | 40.90 |
13 | 0 | 1 | 0 | 1 | 0.5 | 0.5 | 0.5 | 0.5 | 0 | 19.85 |
14 | 1 | 1 | 0 | 0 | 0.5 | 0 | 1 | 1 | 0.5 | 28.75 |
15 | 0 | 0 | 0.5 | 1 | 1 | 0 | 1 | 0 | 0.5 | 30.05 |
16 | 0.5 | 0 | 1 | 0 | 0.5 | 0.5 | 0 | 1 | 0.5 | 19.30 |
17 | 0 | 0.5 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 25.80 |
18 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.50 |
19 | 1 | 0 | 0 | 0.5 | 0 | 1 | 0.5 | 0.5 | 0.5 | 31.60 |
20 | 0 | 1 | 1 | 0 | 1 | 0.5 | 1 | 0.5 | 1 | 27.30 |
21 | 1 | 0.5 | 0 | 1 | 1 | 0.5 | 0 | 0 | 0.5 | 11.10 |
22 | 0.5 | 1 | 0 | 0.5 | 0.5 | 1 | 0 | 0 | 1 | 34.05 |
23 | 0 | 0.5 | 0.5 | 0 | 0.5 | 1 | 0.5 | 1 | 0.5 | 17.35 |
24 | 1 | 1 | 0.5 | 1 | 0 | 0 | 0.5 | 1 | 1 | 28.65 |
25 | 0.5 | 1 | 1 | 1 | 1 | 1 | 0.5 | 0 | 0.5 | 14.35 |
26 | 1 | 0 | 1 | 1 | 0.5 | 1 | 1 | 0.5 | 0 | 7.60 |
27 | 0.5 | 0.5 | 0 | 0 | 1 | 0 | 0.5 | 0.5 | 1 | 23.80 |
表1 高效菌群对原油的降解率
Table 1 Degradation rate of high-efficiency bacteria on crude oil
实验组Experimental group | SF1 | SF2 | SF3 | SF4 | SF5 | SF6 | SF7 | SF8 | SF9 | 降解率Degradation rate/% |
---|---|---|---|---|---|---|---|---|---|---|
1 | 0 | 0 | 1 | 0.5 | 0.5 | 0 | 0.5 | 0 | 0 | 30.20 |
2 | 1 | 0.5 | 1 | 0 | 0 | 0.5 | 0.5 | 0 | 0 | 38.00 |
3 | 0.5 | 0.5 | 1 | 0.5 | 0 | 0 | 1 | 0.5 | 0.5 | 41.25 |
4 | 0.5 | 0.5 | 0.5 | 1 | 0.5 | 0 | 0 | 0.5 | 0 | 43.60 |
5 | 1 | 0 | 0.5 | 0 | 1 | 1 | 0 | 0.5 | 1 | 37.10 |
6 | 0.5 | 1 | 0.5 | 0 | 0 | 1 | 1 | 0 | 0 | 31.1 |
7 | 0 | 1 | 0.5 | 0.5 | 0 | 0.5 | 0 | 0.5 | 0.5 | 34.45 |
8 | 0.5 | 0 | 0.5 | 0.5 | 1 | 0.5 | 0.5 | 1 | 0 | 28.60 |
9 | 1 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 1 | 0 | 1 | 57.88 |
10 | 1 | 1 | 1 | 0.5 | 1 | 0 | 0 | 1 | 0 | 24.90 |
11 | 0.5 | 0 | 0 | 1 | 0 | 0.5 | 1 | 1 | 1 | 28.55 |
12 | 0 | 0.5 | 0 | 0.5 | 1 | 1 | 1 | 1 | 0 | 40.90 |
13 | 0 | 1 | 0 | 1 | 0.5 | 0.5 | 0.5 | 0.5 | 0 | 19.85 |
14 | 1 | 1 | 0 | 0 | 0.5 | 0 | 1 | 1 | 0.5 | 28.75 |
15 | 0 | 0 | 0.5 | 1 | 1 | 0 | 1 | 0 | 0.5 | 30.05 |
16 | 0.5 | 0 | 1 | 0 | 0.5 | 0.5 | 0 | 1 | 0.5 | 19.30 |
17 | 0 | 0.5 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 25.80 |
18 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.50 |
19 | 1 | 0 | 0 | 0.5 | 0 | 1 | 0.5 | 0.5 | 0.5 | 31.60 |
20 | 0 | 1 | 1 | 0 | 1 | 0.5 | 1 | 0.5 | 1 | 27.30 |
21 | 1 | 0.5 | 0 | 1 | 1 | 0.5 | 0 | 0 | 0.5 | 11.10 |
22 | 0.5 | 1 | 0 | 0.5 | 0.5 | 1 | 0 | 0 | 1 | 34.05 |
23 | 0 | 0.5 | 0.5 | 0 | 0.5 | 1 | 0.5 | 1 | 0.5 | 17.35 |
24 | 1 | 1 | 0.5 | 1 | 0 | 0 | 0.5 | 1 | 1 | 28.65 |
25 | 0.5 | 1 | 1 | 1 | 1 | 1 | 0.5 | 0 | 0.5 | 14.35 |
26 | 1 | 0 | 1 | 1 | 0.5 | 1 | 1 | 0.5 | 0 | 7.60 |
27 | 0.5 | 0.5 | 0 | 0 | 1 | 0 | 0.5 | 0.5 | 1 | 23.80 |
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