生物技术通报 ›› 2021, Vol. 37 ›› Issue (4): 107-115.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1094

• 研究报告 • 上一篇    下一篇

高效原油污染降解菌的筛选、鉴定及菌群的构建

朱淑芳(), EHENEDEN Iyobosa, 宁海军, 尚洁昊, 李武阳, 孟宪刚()   

  1. 兰州交通大学化学与生物工程学院,兰州 730070
  • 收稿日期:2020-08-29 出版日期:2021-04-26 发布日期:2021-05-13
  • 作者简介:朱淑芳,女,硕士研究生,研究方向:含油污泥的处置;E-mail:1726457380@qq.com

Screening and Identification of Highly Efficient Crude Oil-degrading Bacteria and Construction of Bacterial Consortium

ZHU Shu-fang(), EHENEDEN Iyobosa, NING Hai-jun, SHANG Jie-hao, LI Wu-yang, MENG Xian-gang()   

  1. School of Chemical and Biological Engineering,Lanzhou Jiaotong University,Lanzhou 730070
  • Received:2020-08-29 Published:2021-04-26 Online:2021-05-13

摘要:

为获得高效原油降解菌株,从山东某油田的含油污泥中和实验室自有菌剂以及原油污染的土壤中分离筛选得到9株微生物,分别编号SF1、SF2、SF3、SF4、SF5、SF6、SF7、SF8和SF9。研究表明,9株菌对原油均有一定的降解能力。通过16S rRNA鉴定表明:SF1为中间苍白杆菌属(Ochrobactrum intermedium),SF2、SF8属于肠杆菌属(Enterobacter sp.),SF3、SF7为不动杆菌属(Acinetobacter sp.),SF4、SF5、SF6、SF9属于假单胞菌菌属(Pseudomonas sp.)。其中,菌株SF1降解能力最强,降解率达48.73%,菌株SF2、SF7、SF9的降解能力较强,降解率分别为41.90%、45.35%、32.25%,其他菌株的降解率也高于20%。通过正交实验得出,由菌株SF1、SF2、SF3、SF4、SF5、SF6、SF7和SF9且添加量为2∶1∶1∶1∶1∶1∶2∶2组成的菌群对原油的降解效果优于单个菌株,该菌群为最佳高效菌群,降解率达57.88%。

关键词: 原油污染, 高效降解菌, 筛选, 鉴定, 菌群构建

Abstract:

In order to obtain efficient crude oil-degrading strains,9 strains of microorganisms were isolated and screened from the oily sludge of an oil field in Shandong,laboratory’s own bacterial agents and crude oil contaminated soil,which were numbered SF1,SF2,SF3,SF4,SF5,SF6,SF7,SF8 and SF9. Studies have shown that 9 strains presented certain abilities of degrading crude oil. Via 16S rRNA identification,SF1 belonged to Ochrobactrum intermedium,SF2 and SF8 belonged to Enterobacter sp,SF3 and SF7 belonged to Acinetobacter sp,SF4,SF5,SF6 and SF9 belonged to Pseudomonas sp. Among them,strain SF1 has the strongest degradability,with a degradation rate of 48.73%,strains SF2,SF7,and SF9 showed strong degradability,with degradation rates of 41.90%,45.35%,and 32.25%,respectively. The degradation rates of other strains were higher than 20%. Through orthogonal experiments,it is concluded that the bacterial flora composed of strains SF1,SF2,SF3,SF4,SF5,SF6,SF7and SF9 with the addition amount of 2∶1∶1∶1∶1∶1∶2∶2 has an effect on crude oil,and the degradation effect is better than that by a single strain,and this flora is the best efficient one,with a degradation rate of 57.88%.

Key words: crude oil pollution, highly efficient degrading bacteria, screening, identification, flora construction