生物技术通报 ›› 2021, Vol. 37 ›› Issue (8): 152-161.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0130
王小河(), 辜夕容(), 祁顺菊, 李杰, 崔瑶, 李得霞, 杨莉荟
收稿日期:
2021-02-01
出版日期:
2021-08-26
发布日期:
2021-09-10
作者简介:
王小河,男,硕士研究生,研究方向:经济林开发利用与林业生物技术;E-mail: 基金资助:
WANG Xiao-he(), GU Xi-rong(), QI Shun-ju, LI Jie, CUI Yao, LI De-xia, YANG Li-hui
Received:
2021-02-01
Published:
2021-08-26
Online:
2021-09-10
摘要:
探究巴山榧树枝和叶提取物的抗氧化能力、抑菌活性和枝叶挥发性成分,为开发利用巴山榧树的食用或药用价值提供科学依据。利用90%乙醇超声提取巴山榧树枝和叶,采用自由基清除率测定抗氧化能力,牛津杯法分析抑菌活性,顶空固相微萃取-气相色谱质谱联用法检测枝叶挥发性成分及其相对含量。结果发现,巴山榧树枝和叶的提取物对DPPH、ABTS+和羟基自由基有不同程度的清除作用,以开州区的巴山榧树枝提取物对DPPH和ABTS+自由基清除率最高,分别达到39.9%和95.3%;开州区巴山榧树叶提取物的羟基自由基清除率最高,达到69.8%。巴山榧树枝和叶的提取物显著抑制大肠杆菌、枯草芽孢杆菌和金黄色葡萄球菌的生长和繁殖,以开州区巴山榧树枝提取物对枯草芽孢杆菌的抑菌活性最强,抑菌圈直径达到26.9 mm。巴山榧树枝和叶的挥发性成分中98%以上为萜烯类化合物,以单萜和倍半萜为主。巴山榧树枝和叶提取物具有较强的抗氧化能力与抑菌活性,其枝叶挥发性成分主要为单萜和倍半萜。
王小河, 辜夕容, 祁顺菊, 李杰, 崔瑶, 李得霞, 杨莉荟. 巴山榧树枝和叶提取物的抗氧化能力、抑菌活性与挥发性成分[J]. 生物技术通报, 2021, 37(8): 152-161.
WANG Xiao-he, GU Xi-rong, QI Shun-ju, LI Jie, CUI Yao, LI De-xia, YANG Li-hui. Antioxidant Activity,Antibacterial Activity and Volatile Components of Extracts from the Branches and Leaves of Torreya fargesii Franch.[J]. Biotechnology Bulletin, 2021, 37(8): 152-161.
图1 巴山榧树枝和叶提取物的DPPH自由基清除率 图中不同字母表示差异显著(P < 0.05)。下同
Fig.1 Scavenging rate of DPPH free radical by the extracts of branches and leaves of T. fargesii Franch. Different letters in the figure indicate significant differences(P <0.05). The same below
化学成分 Chemical components | 分子式 Molecular formula | 分子量 Molecular weight | 枝含量 Content of branches/% | 叶含量 Content of leaves/% |
---|---|---|---|---|
α-蒎烯 α-pinene | C10H16 | 136 | 43.17 | 40.45 |
2-亚甲基-6,6-二甲基-[3.1.1]-二环庚烷 Bicyclo[3.1.1]heptane,6,6-dimethyl-2-methylene | C10H16 | 136 | — | 1.77 |
β-月桂烯 β-Myrcene | C10H16 | 136 | 1.94 | 1.49 |
2-亚甲基-7,7-二甲基-二环[2.2.1]庚烷 Bicyclo[2.2.1]heptane,7,7-dimethyl-2-methylene | C10H16 | 136 | — | 0.65 |
1,4-二甲基-4-乙烯基-环己烯 Cyclohexene,4-ethenyl-1,4-dimethyl | C10H16 | 136 | 2.45 | — |
β-水芹烯β-Phellandrene | C10H16 | 136 | 0.79 | — |
1,7,7-三甲基-二环庚醇乙酸酯 Bicyclo[2.2.1]heptan-2-ol,1,7,7-trimethyl,acetate | C12H20O2 | 196 | — | 1.6 |
乙酸异龙脑酯Isobornyl acetate | C12H20O2 | 196 | 0.86 | — |
石竹烯 Caryophyllene | C15H24 | 204 | 0.93 | 7.02 |
α-石竹烯 α-Caryophyllene | C15H24 | 204 | 6.03 | 6.8 |
α-甜没药烯 α-Bisabolene | C15H24 | 204 | 13.13 | 1.44 |
杜松萜烯 Cadinene | C15H24 | 204 | 13.18 | 10.05 |
α-摩勒烯 α-Muurolene | C15H24 | 204 | 0.84 | 0.77 |
α-荜澄茄油烯 α-Cubebene | C15H24 | 204 | — | 1.28 |
5-亚甲基-1-甲基-8-(1-甲基乙基)-1,6-环癸二烯 1,6-Cyclodecadiene,1-methyl-5-methylene-8-(1-methylethyl) | C15H24 | 204 | 16.68 | 26.68 |
表1 巴山榧树枝和叶中的挥发性成分及其相对含量
Table 1 Volatile components and their relative contents in the branches and leaves of T. fargesii Franch.
化学成分 Chemical components | 分子式 Molecular formula | 分子量 Molecular weight | 枝含量 Content of branches/% | 叶含量 Content of leaves/% |
---|---|---|---|---|
α-蒎烯 α-pinene | C10H16 | 136 | 43.17 | 40.45 |
2-亚甲基-6,6-二甲基-[3.1.1]-二环庚烷 Bicyclo[3.1.1]heptane,6,6-dimethyl-2-methylene | C10H16 | 136 | — | 1.77 |
β-月桂烯 β-Myrcene | C10H16 | 136 | 1.94 | 1.49 |
2-亚甲基-7,7-二甲基-二环[2.2.1]庚烷 Bicyclo[2.2.1]heptane,7,7-dimethyl-2-methylene | C10H16 | 136 | — | 0.65 |
1,4-二甲基-4-乙烯基-环己烯 Cyclohexene,4-ethenyl-1,4-dimethyl | C10H16 | 136 | 2.45 | — |
β-水芹烯β-Phellandrene | C10H16 | 136 | 0.79 | — |
1,7,7-三甲基-二环庚醇乙酸酯 Bicyclo[2.2.1]heptan-2-ol,1,7,7-trimethyl,acetate | C12H20O2 | 196 | — | 1.6 |
乙酸异龙脑酯Isobornyl acetate | C12H20O2 | 196 | 0.86 | — |
石竹烯 Caryophyllene | C15H24 | 204 | 0.93 | 7.02 |
α-石竹烯 α-Caryophyllene | C15H24 | 204 | 6.03 | 6.8 |
α-甜没药烯 α-Bisabolene | C15H24 | 204 | 13.13 | 1.44 |
杜松萜烯 Cadinene | C15H24 | 204 | 13.18 | 10.05 |
α-摩勒烯 α-Muurolene | C15H24 | 204 | 0.84 | 0.77 |
α-荜澄茄油烯 α-Cubebene | C15H24 | 204 | — | 1.28 |
5-亚甲基-1-甲基-8-(1-甲基乙基)-1,6-环癸二烯 1,6-Cyclodecadiene,1-methyl-5-methylene-8-(1-methylethyl) | C15H24 | 204 | 16.68 | 26.68 |
[1] | 中国科学院中国植物志编辑委员会. 中国植物志-第七卷[M]. 北京: 科学出版社, 1978: 462. |
Delectis Florae Reipublicae Popularis Sinicae Agendae Academiae Sinicae Edita. Flora Reipublicae Popularis Sinicae[M]. Beijing: Science Press, 1978: 462. | |
[2] | 陈振德, 郑汉臣, 傅秋华, 等. 国产榧属植物种子油含量及其脂肪酸测定[J]. 中国中药杂志, 1998, 23(8):8-9, 34, 63. |
Chen ZD, Zheng HC, Fu QH, et al. Determination of oil contents and fatty acids in seeds of Torreya arn. China[J]. China J Chin Mater Med, 1998, 23(8):8-9, 34, 63. | |
[3] | 周先容, 余岩, 周颂东, 等. 巴山榧树地理分布格局及潜在分布区[J]. 林业科学, 2012, 48(2):1-8. |
Zhou XR, Yu Y, Zhou SD, et al. Geographic distribution and potential distribution of Torreya fargesii[J]. Sci Silvae Sin, 2012, 48(2):1-8. | |
[4] | 江波, 周先容, 尚进, 等. 中国特有植物巴山榧树的种群结构与动态[J]. 生态学报, 2018, 38(3):1016-1027. |
Jiang B, Zhou XR, Shang J, et al. Population structure and dynamics of Torreya fargesii Franch., a plant endemic to China[J]. Acta Ecol Sin, 2018, 38(3):1016-1027. | |
[5] | 周先容, 尚进, 王庆, 等. 四川花萼山巴山榧树不同世代种群的遗传多样性[J]. 分子植物育种, 2019, 17(6):2061-2068. |
Zhou XR, Shang J, Wang Q, et al. Genetic diversity of different generations of Torreya fargesii population in hua’e mountain, Sichuan Province[J]. Mol Plant Breed, 2019, 17(6):2061-2068. | |
[6] | 贾晓会. 香榧叶化学成分及抗氧化、抗疲劳活性研究[D]. 杭州:浙江农林大学, 2017. |
Jia XH. Study on the chemical analysis and antioxidant, anti-fatigue activity of Torreya leaves[D]. Hangzhou:Zhejiang A & F University, 2017. | |
[7] | 贾姗姗. 不同品种榧树化学成分分析及生物活性研究[D]. 杭州:浙江农林大学, 2016. |
Jia SS. Study on the chemical analysis and biological activity of Torreya grandis varieties[D]. Hangzhou:Zhejiang A & F University, 2016. | |
[8] | 段芳芳. 香榧假种皮化学成分及生物活性研究[D]. 杭州:浙江农林大学, 2018. |
Duan FF. Study on chemical constituents separation and biological activity of Torreya grandis aril[D]. Hangzhou:Zhejiang A & F University, 2018. | |
[9] | 陈振德, 陈志良, 侯连兵, 等. 香榧子油对实验性动脉粥样硬化形成的影响[J]. 中药材, 2000, 23(9):551-553. |
Chen ZD, Chen ZL, Hou LB, et al. The preventive effect of the oil from the seed of Torreya grandis cv. merrillii on experimental atherosclerosis in rats[J]. J Chin Med Mater, 2000, 23(9):551-553. | |
[10] | 陈仁通, 张与欢, 方圣鼎. 长叶榧叶中对人体DNA多聚酶β的抑制成分[J]. 中草药, 1997(12):707-710. |
Chen RT, Zhang YH, Fang SD. Inhibitors of human DNA polymerase βIsolated from jack Torreya(Torreya jackii)[J]. Chin Tradit Herb Drugs, 1997(12):707-710. | |
[11] | 王鸿, 郭涛, 应国清. 榧属植物活性成分与药理作用研究进展[J]. 中草药, 2007, 38(11):1747-1750. |
Wang H, Guo T, Ying GQ. Advances in studies on active constituents and their pharmacological activities for plants of Torreya Arn[J]. Chin Tradit Herb Drugs, 2007, 38(11):1747-1750. | |
[12] | 张虹, 王洪泉, 陈振德. 日本榧叶挥发油成分抗菌抗真菌作用研究[J]. 中国药师, 2002, 5(9):549. |
Zhang H, Wang HQ, Chen ZD. Antibacterial and antifungal effects of essential oil from Torreya nucifera leaves[J]. China Pharm, 2002, 5(9):549. | |
[13] | 张喜萍. 日本榧树树皮和祁洲漏芦根的化学成分研究[D]. 石家庄:河北医科大学, 2009. |
Zhang XP. Chemical study on the Torreya nucifera(L.)sieb et zucc. barks[D]. Shijiazhuang:Hebei Medical University, 2009. | |
[14] |
Endo Y, Osada Y, Kimura F, et al. Effects of Japanese Torreya(Torreya nucifera)seed oil on lipid metabolism in rats[J]. Nutrition, 2006, 22(5):553-558.
doi: 10.1016/j.nut.2005.08.012 URL |
[15] |
Zhou XR, Shang J, Wang JH, et al. Antioxidant activity of extracts from the aril of Torreya fargesii Franch. And its protection on the oxidation of DHA algal oil[J]. Cyta J Food, 2018, 16(1):381-389.
doi: 10.1080/19476337.2017.1409270 URL |
[16] | 包立军, 彭云武, 殷创, 等. 水解方式对黄茧蚕丝多肽抗氧化活性的影响[J]. 食品研究与开发, 2017, 38(17):14-16, 30. |
Bao LJ, Peng YW, Yin C, et al. Studies on polypeptide antioxidant activity of natural yellow cocoon by different hydrolysis methods[J]. Food Res Dev, 2017, 38(17):14-16, 30. | |
[17] | 周晶, 桂雨豪, 龚盛昭. 茶籽壳发酵物的抗氧化活性及其对细胞内氧化损伤抑制作用[J]. 林产化学与工业, 2019, 39(4):105-112. |
Zhou J, Gui YH, Gong SZ. Antioxidant activity of tea seed shell fermentation extracts and its inhibitory effect on intracellular oxidative damage[J]. Chem Ind For Prod, 2019, 39(4):105-112. | |
[18] | 叶颖晓, 张朋展, 王丽, 等. 不同提取方法对豫产白花蛇舌草多糖及抗氧化活性的影响[J]. 天然产物研究与开发, 2019, 31(7):1138-1146. |
Ye YX, Zhang PZ, Wang L, et al. Effects of different extraction methods on polysaccharide and its antioxidant characteristics of Hedyotis diffusa Willd in Henan Province[J]. Nat Prod Res Dev, 2019, 31(7):1138-1146. | |
[19] | 刘婵婵. 丁香酚对多重耐药大肠杆菌的抑菌活性及其机制研究[D]. 西安:陕西科技大学, 2019. |
Liu CC. Study on antimicrobial activity and mechanism of eugenol against multi-drug resistant Escherichia coli[D]. Xi’an:Shaanxi University of Science & Technology, 2019. | |
[20] | 沈勇, 刘文茹, 梅俊, 等. 花鲈鱼肠道中产细菌素粪肠球菌的筛选和抑菌效果研究[J]. 食品与发酵工业, 2019, 45(14):15-19. |
Shen Y, Liu WR, Mei J, et al. Identification and antibacterial activity of bacteriocin-producing Enterococcus faecalis isolated from Lateolabrax japonocus intestine[J]. Food Ferment Ind, 2019, 45(14):15-19. | |
[21] | 杨占南, 何前松, 彭全材, 等. 顶空固相微萃取-气相色谱-质谱法测定疏毛吴茱萸挥发性化学成分[J]. 时珍国医国药, 2010, 21(10):2501-2505. |
Yang ZN, He QS, Peng QC, et al. Analysis of the volatile phytochemical compositions of fruits from Evodia ruatecarpa(juss. )bneht. var. bodinieri(dode)Huang by HS-SPME-GC-MS[J]. Lishizhen Med Mater Med Res, 2010, 21(10):2501-2505. | |
[22] | 孙丽超, 李淑英, 王凤忠, 等. 萜类化合物的合成生物学研究进展[J]. 生物技术通报, 2017, 33(1):64-75. |
Sun LC, Li SY, Wang FZ, et al. Research progresses in the synthetic biology of terpenoids[J]. Biotechnol Bull, 2017, 33(1):64-75. | |
[23] | 孙永. 三叶青化学成分及其抗氧化和抗癌活性的研究[D]. 南昌:南昌大学, 2018. |
Sun Y. Chemical composition, activities of antioxidation and anticancer of Tetrastigma hemsleyanum Diels et gilg[D]. Nanchang:Nanchang University, 2018. | |
[24] |
Sena LA, Chandel NS. Physiological roles of mitochondrial reactive oxygen species[J]. Mol Cell, 2012, 48(2):158-167.
doi: 10.1016/j.molcel.2012.09.025 URL |
[25] |
García-Lomillo J, Gonzalez-Sanjose ML, del Pino-García R, et al. Antioxidant effect of seasonings derived from wine pomace on lipid oxidation in refrigerated and frozen beef patties[J]. LWT, 2017, 77:85-91.
doi: 10.1016/j.lwt.2016.11.038 URL |
[26] | 李艳美, 李芳, 粟有志, 等. QuEChERS前处理结合高效液相色谱-串联质谱法同时测定酵母产品中4种抗氧化剂和5种防腐剂[J]. 食品与发酵工业, 2019, 45(12):215-221. |
Li YM, Li F, Su YZ, et al. Simultaneous determination of antioxidants and p-hydroxybenzoate antiseptics in yeast products using QuEChERS and high-performance liquid chromatography-tandem mass spectrometry[J]. Food Ferment Ind, 2019, 45(12):215-221. | |
[27] | 类红梅, 罗欣, 毛衍伟, 等. 天然抗氧化剂的功能及其在肉与肉制品中的应用研究进展[J]. 食品科学, 2020, 41(21):267-277. |
Lei HM, Luo X, Mao YW, et al. A review of the functions and application of natural antioxidants in meat and meat products[J]. Food Sci, 2020, 41(21):267-277. | |
[28] |
Shahidi F, Zhong Y. Novel antioxidants in food quality preservation and health promotion[J]. Eur J Lipid Sci Technol, 2010, 112(9):930-940.
doi: 10.1002/ejlt.201000044 URL |
[29] | 李春焕, 王晓琴, 曾秋梅. 植物油脂氧化过程及机理、检测技术以及影响因素研究进展[J]. 食品与发酵工业, 2016, 42(9):277-284. |
Li CH, Wang XQ, Zeng QM. Research progress of the process and mechanism, detemination and influential factors of vegetable lipid oxidation[J]. Food Ferment Ind, 2016, 42(9):277-284. | |
[30] |
Cunha LCM, Monteiro MLG, Lorenzo JM, et al. Natural antioxidants in processing and storage stability of sheep and goat meat products[J]. Food Res Int, 2018, 111:379-390.
doi: S0963-9969(18)30409-5 pmid: 30007699 |
[31] | Zhu MF, Tu ZC, Zhang L, et al. Antioxidant, metabolic enzymes inhibitory ability of Torreya grandis kernels, and phytochemical profiling identified by HPLC-QTOF-MS/MS[J]. J Food Biochem, 2019, 43(12):e13043. |
[32] | 王健, 薛山, 赵国华. 紫苏不同部位精油成分及体外抗氧化能力的比较研究[J]. 食品科学, 2013, 34(7):86-91. |
Wang J, Xue S, Zhao GH. Comparative study on chemical components and in vitro antioxidant capacity of essential oil from different parts of Perilla frutescens[J]. Food Sci, 2013, 34(7):86-91. | |
[33] |
Ortega-Ramirez LA, Rodriguez-Garcia I, Leyva JM, et al. Potential of medicinal plants as antimicrobial and antioxidant agents in food industry:a hypojournal[J]. J Food Sci, 2014, 79(2):R129-R137.
doi: 10.1111/1750-3841.12341 URL |
[34] | 郭佳, 魁永忠, 夏泆斌, 等. 不同品种小茴香果实挥发性成分、多酚、黄酮含量及抗氧化性比较分析[J]. 食品与发酵工业, 2019, 45(8):215-224. |
Guo J, Kui YZ, Xia YB, et al. Comparative analysis of volatile compounds, polyphenols, flavonoids and antioxidant capacities of different Foeniculum vulgare Mill cultivars[J]. Food Ferment Ind, 2019, 45(8):215-224. | |
[35] |
Rufino MDSM, Alves RE, de Brito ES, et al. Bioactive compounds and antioxidant capacities of 18 non-traditional tropical fruits from Brazil[J]. Food Chem, 2010, 121(4):996-1002.
doi: 10.1016/j.foodchem.2010.01.037 URL |
[36] | 陈振德, 郑汉臣, 田美兰, 等. 长叶榧叶挥发油成分分析及其抗菌作用研究[J]. 第二军医大学学报, 1998, 19(2):150-152 |
Chen ZD, Zheng HC, Tian ML, et al. Constituents analysis and antibacterial and antifungous actions of the essential oil in leaves of Torreya jackii Chun[J]. Acad J Second Mil Med Univ, 1998, 19(2):150-152 | |
[37] |
Ji W, Wang W, Zhao X, et al. Homologous recombination within the spike glycoprotein of the newly identified coronavirus may boost cross-species transmission from snake to human[J]. Journal of Medical Virology, 2020, 92(4):433-440
doi: 10.1002/jmv.v92.4 URL |
[38] | 郭阿君. 红皮云杉自然挥发物成分及抑菌作用[J]. 北华大学学报:自然科学版, 2016, 17(6):736-740. |
Guo A. Volatile components of Picea koraiensis and effects on bacteria[J]. J Beihua Univ:Nat Sci, 2016, 17(6):736-740. | |
[39] |
Sperotto ARM, Moura DJ, Péres VF, et al. Cytotoxic mechanism of Piper gaudichaudianum Kunth essential oil and its major compound nerolidol[J]. Food Chem Toxicol, 2013, 57:57-68.
doi: 10.1016/j.fct.2013.03.013 pmid: 23523831 |
[40] | 老莹, 胡文忠, 冯可, 等. 天然抑菌剂的抑菌机理及其在果蔬保鲜中的应用[J]. 食品与发酵工业, 2018, 44(9):288-293. |
Lao Y, Hu WZ, Feng K, et al. Application of natural antimicrobial agents on fruits and vegetables preservation and its mechanism[J]. Food Ferment Ind, 2018, 44(9):288-293. | |
[41] |
Nogueira JHC, Gonçalez E, Galleti SR, et al. Ageratum conyzoides essential oil as aflatoxin suppressor of Aspergillus flavus[J]. Int J Food Microbiol, 2010, 137(1):55-60.
doi: 10.1016/j.ijfoodmicro.2009.10.017 pmid: 19906457 |
[42] | 徐士超, 董欢欢, 曾小静, 等. 萜类植物源农药的筛选及活性研究进展[J]. 林产化学与工业, 2019, 39(1):1-12. |
Xu SC, Dong HH, Zeng XJ, et al. Research progress in screening and bioactivity of terpenoid botanical pesticides[J]. Chem Ind For Prod, 2019, 39(1):1-12. | |
[43] | 李然然. β-石竹烯对脑缺血再灌注大鼠的作用及其机制研究[D]. 重庆:重庆医科大学, 2016. |
Li RR. Effects of β-caryophyllene on focal cerebral ischemia reperfusion injury in rats and its mechanism study[D]. Chongqing:Chongqing Medical University, 2016. | |
[44] |
Lija-Escaline J, Senthil-Nathan S, Thanigaivel A, et al. Physiological and biochemical effects of botanical extract from Piper nigrum Linn(Piperaceae)against the dengue vector Aedes aegypti Liston(Diptera:Culicidae)[J]. Parasitol Res, 2015, 114(11):4239-4249.
doi: 10.1007/s00436-015-4662-1 pmid: 26277727 |
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