生物技术通报 ›› 2021, Vol. 37 ›› Issue (10): 72-80.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0124

• 研究报告 • 上一篇    下一篇

一种苯酚降解菌Pseudoxanthomonas sp. BF-6的分离鉴定及其降解特性及途径研究

卫晓博1,3(), 侯颖1,2(), 程豪杰1,2, 秦翠丽1,3, 牛明福1,2, 徐建强4()   

  1. 1.河南科技大学食品与生物工程学院,洛阳 471023
    2.河南科技大学食品与生物工程学院微生物资源开发与利用重点实验室,洛阳 471023
    3.河南省食品微生物工程技术研究中心,洛阳 471023
    4.河南科技大学园艺与植物保护学院,洛阳 471023
  • 收稿日期:2021-01-29 出版日期:2021-10-26 发布日期:2021-11-12
  • 作者简介:卫晓博,男,硕士研究生,研究方向:环境微生物学;E-mail: 1652330512@qq.com
  • 基金资助:
    国家自然科学基金项目(31400098);河南省科技攻关计划项目(202102110071);河南省科技攻关计划项目(172102310435)

Isolation,Identification of Phenol-degrading Pseudoxanthomonas sp. BF-6 and Its Degradation Characteristics and Pathway

WEI Xiao-bo1,3(), HOU Ying1,2(), CHENG Hao-jie1,2, QIN Cui-li1,3, NIU Ming-fu1,2, XU Jian-qiang4()   

  1. 1. College of Food and Bioengineering,Henan University of Science and Technology,Luoyang 471023
    2. College of Food and Bioengineering,Henan University of Science and Technology,Key Laboratory of Microbial Resources Exploitation and Utilization,Luoyang 471023
    3. Henan EngineeringResearch Center of Food Microbiology,Luoyang 471023
    4. College of Horticulture and Plant protection,Henan University of Science and Technology,Luoyang 471023
  • Received:2021-01-29 Published:2021-10-26 Online:2021-11-12

摘要:

苯酚是最常见的有机污染物,为了获得降解苯酚的细菌,并探究其降解苯酚的特性和途径,采用连续富集和稀释平板分离,从农药厂活性污泥中得到一株能以苯酚作为唯一碳源生长的菌株BF-6,通过 16S rRNA 序列分析和生理生化试验将该菌株鉴定为假黄单胞菌(Pseudoxanthomonas sp.)。单因素试验结果表明,菌株BF-6在温度为20-37℃和pH5.0-10.0范围内均可很好的降解苯酚;在此条件下,5%接种量的菌株BF-6在36 h内可将100 mg/L苯酚降解97.79%,96 h可将200 mg/L苯酚降解97.58%。经苯酚诱导后,菌株BF-6细胞粗酶液表现出苯酚羟化酶和邻苯二酚1,2-双加氧酶活性,表明菌株BF-6降解苯酚的途径为邻位途径。本研究为低浓度苯酚的微生物降解提供了新的菌种资源。

关键词: 苯酚, 假黄单胞菌, 分离鉴定, 降解特性, 降解途径

Abstract:

The aim of this work is to isolate phenol-degrading bacteria and to explore the characteristics and pathways of phenol degradation. Continuous enrichment and dilution plate method were applied to isolate the phenol-degrading bacteria,and single factor test was to study the characteristics of phenol degradation. The metabolic pathway of degrading by the strain phenol was deduced by determining the activities of phenol hydroxylase and catechol dioxygenase. A phenol-degrading strain BF-6,growing with phenol as the sole carbon source,was isolated and named as BF-6. Strain BF-6 was identified as Pseudoxanthomonas sp. by using 16S rRNA sequence analysis and physiological and biochemical tests. Strain BF-6 degraded phenol well in the temperature range of 20-37℃ and pH range of 5.0-10.0;under this condition,strain BF-6 with 5% inoculum degraded 100 mg/L phenol by 97.79% in 36 h and 200 mg/L phenol by 97.58% in 96 h. Cell extracts of strain BF-6 showed enzymatic activity of phenol hydroxylase and catechol 1,2-dioxygenase after induction by phenol,indicating that the strain degraded phenol via ortho-pathway. In conclusion,phenol-degrading bacterium BF-6 is identified as Pseudoxanthomonas sp.,and it degrades phenol under a wide range of temperature and pH conditions. Phenol is degraded via ortho-pathway by strain BF-6. This study provides a new strain for microbial-degration of low-concentration phenol.

Key words: phenol, Pseudoxanthomonas sp., isolation and identification, degradation characterization, degradation pathway