两株微生物的Cr（VI）还原特性研究

doi:10.13560/j.cnki.biotech.bull.1985.2021-0136

摘要/Abstract

摘要：

旨为研究2株微生物对Cr（VI）的还原能力及特性。在实验室条件下,通过连续培养,探究2株微生物的还原特性及pH、电子供体对其还原Cr（VI）影响,并结合表征手段研究寡养单胞菌对Cr（VI）的解毒机理。结果显示,寡养单胞菌和胶冻样芽孢杆菌对Cr（VI）的耐受性达400和200 mg/L,中性条件下,72 h内对45.5和18.5 mg/L的Cr（VI）去除率为100%;2株微生物在弱碱性条件下活性较强,在pH=8时,寡养单胞菌和胶冻样芽孢杆菌72 h内对50 mg/L的Cr（VI）去除率达98.1%和49.2%;寡养单胞菌在乙酸钠和乳酸钠的作用下,对50 mg/L的Cr（VI）的去除率可在60 h内达到100%,乳酸钠和葡萄糖可使胶冻样芽孢杆菌72 h内对50 mg/L的Cr（VI）去除率从49.2%提升至61.9%和73.2%;还原产物表征分析表明,寡养单胞菌表面在还原Cr（VI）的过程中吸附Cr（VI）于机体表面,并将Cr（VI）高效还原为Cr（III）。寡养单胞菌和胶冻样芽孢杆菌可作为修复重金属Cr（VI）污染的优质菌种。

关键词: 重金属污染, 微生物, Cr（VI）, 吸附, 还原

Abstract:

This work aims to study the reduction ability and characteristics of two species of microorganisms to Cr（VI）. The abilities of two species of microorganisms to reduce Cr（VI）and the influence of pH and electron donor on its reduction of Cr（VI）were explored by continuous culturing in lab condition,combining characterization methods the detoxification mechanism of Stenotrophomonas sp. was studied The results showed that the tolerance of Stenotrophomonas sp. and Bacillus mucilaginosus to Cr（VI）was 400 and 200 mg/L. Under neutral conditions,the removal rate of 45.5 and 18.5 mg/L Cr（VI）within 72 h was 100%. Under weakly alkaline conditions,the activities of both microorganisms were strong. At pH 8,removal rates of 50 mg/L Cr（VI）by Stenotrophomonas sp. and B. mucilaginosus within 72 h were 98.1% and 49.2% respectively. Sodium acetate and sodium lactate promoted the removal rate of 50 mg/L Cr（VI）by Stenotrophomonas sp. to be 100% within 60 h. Sodium lactate and glucose increased the removal rate of 50 mg/L Cr（VI）by B. mucilaginosus from 49.2% to 61.9% and 73.2% within 72 h,respectively. The analysis of the reduced products showed that Stenotrophomonas sp. adsorbed Cr（VI）on the surface of the body and efficiently reduced Cr（VI）to Cr（III）. Stenotrophomonas sp. and B. mucilaginosus can be used as high quality strain for remediation of heavy metal Cr（VI）pollution.

Key words: heavy metal pollution, microorganism, Cr（VI）, adsorption, reduction

黄玉喜, 程顺利, 赫玲玲, 肖进彬, 任秋鹤, 彭子涵, 周振, 方玉美. 两株微生物的Cr（VI）还原特性研究[J]. 生物技术通报, 2021, 37(10): 63-71.

HUANG Yu-xi, CHENG Shun-li, HE Ling-ling, XIAO Jin-bin, REN Qiu-he, PENG Zi-han, ZHOU Zhen, FANG Yu-mei. Study on the Reduction Characteristics of Cr（VI）by Two Species of Microorganisms[J]. Biotechnology Bulletin, 2021, 37(10): 63-71.

图/表 8

图1 不同Cr（VI）浓度对微生物生长的影响 A:寡养单胞菌;B:胶冻样芽孢杆菌

Fig.1 Effect of different Cr（VI）concentration on the growth of microorganisms A:Stenotrophomonas sp.;B:Bacillus mucilaginosus

图2 两株微生物对Cr（VI）的最大还原量 A:寡养单胞菌;B:胶冻样芽孢杆菌

Fig.2 Maximum reduction of Cr（VI）by two species of microorganisms A:Stenotrophomonas sp.;B:Bacillus mucilaginosus

图3 72 h内Cr（VI）剩余量

Fig.3 Residual amount of Cr（VI）in 72 h

图4 可完全降解最大Cr（VI）浓度

Fig.4 Maximum Cr（VI）concentration that can be completely degraded

图5 初始pH对微生物还原Cr（VI）的影响 A:寡养单胞菌;B:胶冻样芽孢杆菌

Fig.5 Effect of initial pH on the reduction of Cr（VI）by microorganisms A:Stenotrophomonas sp.;B:Bacillus mucilaginosus

图6 不同电子供体对微生物还原Cr（VI）的影响 A:寡养单胞菌;B:胶冻样芽孢杆菌

Fig.6 Effect of different electron donors on the reduction of Cr（VI）by microorganisms A:Stenotrophomonas sp.;B:Bacillus mucilaginosus

图7 寡养单胞菌还原Cr（VI）前、后形貌扫描电镜图 A:还原反应前;B:还原反应后

Fig.7 Scanning electron microscopy before and after reduction of Cr（VI）by Stenotrophomonas sp. A:Before reduction. B:After reduction

图8 寡养单胞菌还原产物的XPS全谱图和Cr2p轨道峰 A:XPS全谱图;B:Cr2p轨道峰

Fig.8 Full range XPS spectra and spectra of Cr2p for the reduction products of Stenotrophomonas sp. A:Full range XPS spectra. B:Spectra of Cr2p

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