水稻不育系稻粒黑粉病抗性QTL的定位

doi:10.13560/j.cnki.biotech.bull.1985.2021-0934

摘要/Abstract

摘要：

为了定位稻粒黑粉病抗性基因,建立水稻抗稻粒黑粉病分子育种技术体系。以对稻粒黑粉病抗性较强的光温敏不育系W9593S和易感稻粒黑粉病、与W9593S不育基因等位的光温敏不育系培矮64S为亲本,通过杂交和多年自交,构建了由183个高世代稳定不育系组成的重组自交系遗传群体。以这个重组自交系遗传群体为材料,利用200个多态性SNP分子标记,通过复合区间作图对稻粒黑粉病抗性QTL进行定位。结果定位到5个稻粒黑粉病抗性QTL,分布在4条染色体上,表型贡献率为7.26%-13.64%,其中,qRRKS-11和qRRKS-8的表型贡献率较大,分别为13.64%和12.07%,定位区间的遗传距离分别为0.9和1.4 cM。水稻对稻粒黑粉病的抗性表现为数量性状遗传,通过精细定位和克隆主效QTL,可以实现抗稻粒黑粉病不育系的分子标记辅助育种。

关键词: 水稻, 不育系, 稻粒黑粉病抗性, QTL定位

Abstract:

In order to locate the resistance gene of rice kernel smut and establish the molecular breeding system for resistance to rice kernel smut,a RIL（recombinant inbred line）population consisting of 183 high-generation stable male sterile lines was constructed by hybridization and multi-year self-crossing using W9593S,a PTGMS（photo- and thermo-sensitive genic male sterile）line that has strong resistance to rice kernel smut,and Pei'ai 64S,a PTGMS line that has the same sterile allele of W9593S and is susceptible to rice kernel smut,as parent lines. With the RIL population as material,the QTL for resistance to rice kernel smut was mapped by composite interval mapping using 200 polymorphic SNP（single nucleotide polymorphism）molecular markers. The result showed that,5 QTLs with resistance to rice kernel smut were located on 4 chromosomes with phenotypic contribution rates ranging from 7.26% to 13.64%. Among them,qRRKS-11 and qRRKS-8 had the highest phenotypic contribution rates of 13.64% and 12.07%,respectively,and the genetic distances of qRRKS-11 and qRRKS-8 were 0.9 and 1.4 cM,respectively. The resistance to kernel smut is characterized by quantitative trait inheritance in rice,and the molecular marker-assisted breeding system of rice sterile lines resistant to kernel smut may be achieved by fine mapping and cloning of major QTL.

Key words: rice（Oryza sativa）, male sterile line, resistance to rice kernel smut, QTL mapping

杨益善, 孙平勇, 余木兰. 水稻不育系稻粒黑粉病抗性QTL的定位[J]. 生物技术通报, 2022, 38(3): 16-21.

YANG Yi-shan, SUN Ping-yong, YU Mu-lan. QTL Mapping for Resistance to Rice Kernel Smut of Male Sterile Line[J]. Biotechnology Bulletin, 2022, 38(3): 16-21.

图/表 4

图1 RIL群体的稻粒黑粉病粒率频次分布

Fig.1 Frequency distribution of rice kernel smut rate in RIL population

图2 亲本W9593S、培矮64S间差异SNP标记染色体分布绿色表示水稻染色体,蓝色线条表示差异SNP标记,染色体上的数字表示标记数

Fig. 2 Chromosome distribution of differential SNP marker between parents W9593S and Pei'ai 64 S The green indicates the rice chromosome and the blue line indicates the differential SNP marker,and the number on the top of the chromosome refers to the number of markers

图3 稻粒黑粉病抗性QTL在染色体上的分布

Fig. 3 Chromosome distribution of QTL for resistance to rice kernel smut

表1 稻粒黑粉病抗性QTL的定位结果

Table 1 Mapping results of QTL for resistance to rice kernel smut

QTL	染色体 Chromosome	连锁标记区间 Linkage marker interval	遗传距离 Genetic distance/cM	加性效应^* Additive effect	表型贡献率 Phenotypic contribution rate/%	LOD值LOD value
qRRKS-1.1	1	K_010401-K_010402	20.5	-7.04	10.89	3.08
qRRKS-1.2	1	K_010403-K_010365	1.6	-6.17	8.03	3.29
qRRKS-4	4	K_040249-K_040266	34.8	-5.75	7.26	2.51
qRRKS-8	8	K_080131-K_080138	1.4	-7.41	12.07	5.13
qRRKS-11	11	K_110403-K_110016	0.9	7.87	13.64	5.82

参考文献 19

[1]	Chen Y, Yang X, Yao J, et al. Simple and rapid detection of Tilletia horrida causing rice kernel smut in rice seeds[J]. Sci Rep, 2016, 6:33258. doi: 10.1038/srep33258 pmid: 27624858
[2]	宗寿余, 吕川根, 姚克敏, 等. 10个水稻新光温敏核不育系粒黑粉病调查分析[J]. 江苏农业科学, 2004, 32(3):18-19.
	Zong SY, Lv CG, Yao KM, et al. Investigation and analysis of grain smut in 10 new photo- and thermo-sensitive genic male sterile lines in rice[J]. Jiangsu Agric Sci, 2004, 32(3):18-19.
[3]	王爱军, 殷得所, 富蓉, 等. 78个水稻不育系对稻粒黑粉病的抗性评价[J]. 植物病理学报, 2018, 48(2):207-212.
	Wang AJ, Yin DS, Fu R, et al. Evaluation of resistance to rice kernel smut in seventy-eight species of rice sterile lines[J]. Acta Phytopathol Sin, 2018, 48(2):207-212.
[4]	Takahashi Y. On Ustilago virens cooke and a new species of Tilletia parasitic on rice-plant[J]. Shokubutsugaku Zasshi, 1896,10 (109):en16-en20. DOI: 10.15281/jplantres1887.10.109_16. doi: 10.15281/jplantres1887.10.109_16
[5]	王中康, 欧阳秩. 稻粒黑粉菌生物学特性研究[J]. 西南农业大学学报, 1989, 11(4):331-335.
	Wang ZK, Ouyang Z. Studies on the pathogenic biology of the kernel smut of rice[J]. J Southwest Agric Univ, 1989, 11(4):331-335.
[6]	Elshafey RAS. Biology of rice kernel smut disease causal organism Tilletia barclayana and its molecular identification[J]. Journal of Phytopathology and Pest Management, 2018, 5(2):108-128.
[7]	朱建清, 周开达, 陶家凤. 稻粒黑粉菌侵染水稻不育系的细胞学研究[J]. 西南农业学报, 1998, 11(1):67-72.
	Zhu JQ, Zhou KD, Tao JF. Cytological study on Neovossia horrida infection in male sterile lines of rice[J]. Southwest China J Agric Sci, 1998, 11(1):67-72.
[8]	陶家凤, 周开达, 朱建清. 稻粒黑粉菌对水稻不育系的侵染过程[J]. 西南农业学报, 1998, 11(2):68-72.
	Tao JF, Zhou KD, Zhu JQ. Infection process of Neovossia horrida in male sterile rice[J]. Southwest China J Agric Sci, 1998, 11(2):68-72.
[9]	陈毓苓, 陆凡, 王法明, 等. 杂交水稻粒黑粉病发生规律研究[J]. 江苏农业科学, 1992, 20(4):33-35.
	Chen YL, Lu F, Wang FM, et al. Studies on the occurrence regularity of grain smut in hybrid rice[J]. Jiangsu Agric Sci, 1992, 20(4):33-35.
[10]	滕彬, 李光尧, 腾久生, 等. 培矮64S系列组合制种稻粒黑粉病发生原因分析及防治对策[J]. 种子, 2002, 21(6):81-81, 83
	Teng B, Li GY, Teng JS, et al. Analysis on the causes and control measures of rice kernel smut in seed production of the combinations of Pei’ai 64S[J]. Seed, 2002, 21(6):81-81, 83
[11]	柯玲, 廖燕俸. 2016 年尤溪县烟后杂交制种稻粒黑粉病发生原因及防控策略[J]. 中国农业信息, 2017(1):74-76.
	Ke L, Liao YF. Occurrence causes and control strategies of rice kernel smut in seed production after tabacoo of hybrid rice in Youxi County, 2016. [J]. China Agric Inf, 2017(1):74-76.
[12]	Honkura R, Miura M, Miura Y. The damage and the control of rice kernel smut caused by Tilletia horrida Takahashi[J]. Ann Rept Plant Prot North Japan, 1990, 41:43-46.
[13]	Honkura R, Osada S. The time of chemicals application for control of kernel smut of rice plant[J]. Ann Rept Plant Prot North Japan, 1993, 44:10.
[14]	Tsuda M, Sasahara M, Ohara T, et al. Optimal application timing of simeconazole granules for control of rice kernel smut and false smut[J]. J Gen Plant Pathol, 2006, 72(5):301-304. doi: 10.1007/s10327-006-0288-6 URL
[15]	Ei-kazzaz MK, Salem EA, Ghoneim KE, et al. Biocontrol of Tilletia barclayana, the causal agent of kernel smut disease in rice[J]. Egyptian Journal of Biological Pest Control, 2015, 25(3):535-544.
[16]	El-Kazzaz MK, Salem EA, Ghoneim KE, et al. Integrated control of rice kernel smut disease using plant extracts and salicylic acid[J]. Arch Phytopathol Plant Prot, 2015, 48(8):664-675. doi: 10.1080/03235408.2015.1092202 URL
[17]	陆建国, 张戟, 朱艳, 等. 不同类型水稻不育系上稻粒黑粉病的发生初探[J]. 江苏农业科学, 2001, 29(5):41-42.
	Lu JG, Zhang J, Zhu Y, et al. Preliminary study on the occurrence of rice kernel smut on different types of rice sterile lines[J]. Jiangsu Agric Sci, 2001, 29(5):41-42.
[18]	李华全. 论水稻制种稻粒黑粉病的发病规律及防治技术[J]. 种子, 2000, 19(5):55-56.
	Li HQ. On the regularity of occurrence and prevention of rice kernel smut in seed production of hybrid rice[J]. Seed, 2000, 19(5):55-56.
[19]	Mc Couch SR, Cho YG, Yano M, et al. Report on QTL nomenclature[J]. Rice Genetics Newsletter, 1997, 14:11-13.