生物技术通报 ›› 2022, Vol. 38 ›› Issue (3): 9-15.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0802

• 研究报告 • 上一篇    下一篇

水稻响应盐胁迫关键转录因子的鉴定

张斌(), 杨昕霞   

  1. 湖南科技学院 湖南省银杏工程技术研究中心,永州 425199
  • 收稿日期:2021-06-24 出版日期:2022-03-26 发布日期:2022-04-06
  • 作者简介:张斌,男,博士,副教授,研究方向:植物发育生物学;E-mail: zhangbin27104@163.com
  • 基金资助:
    湖南省自然科学基金项目(2020JJ4030);湖南省创新平台与人才计划(2020NK4222)

Identification of Key Transcription Factors in Response to Salt Stress in Rice

ZHANG Bin(), YANG Xin-xia   

  1. Hunan University of Science and Engineering,Hunan Provincial Engineering Research Center for Ginkgo biloba,Yongzhou 425199
  • Received:2021-06-24 Published:2022-03-26 Online:2022-04-06

摘要:

盐胁迫是影响水稻生产的重要非生物胁迫之一。本研究以盐胁迫条件下的水稻转录组数据为材料,通过HTSeq和DESeq软件分析转录因子在转录组水平上的表达变化。主要结果如下:水稻在盐胁迫1、3和6 h三个时间点共有26个相同的差异表达转录因子,通过蛋白互作筛选出14个基因组成的重要模块;重要模块基因GO分析主要富集在ATP结合功能、对胁迫反应的过程以及细胞质膜囊泡组分上,KEGG分析主要富集在内质网蛋白质加工和内吞作用的通路上;同时鉴定出受盐胁迫诱导表达的关键热激转录因子基因Os03g0745000Os06g0553100。推测这两个基因可能通过调控HSP70基因的转录表达,影响蛋白质折叠和组装,从而在水稻响应盐胁迫的过程中发挥作用。该研究为后续进行水稻遗传改良提供了候选基因,对进一步认识耐盐机制提供科学启示。

关键词: 水稻, 关键转录因子, 盐胁迫, 蛋白互作, 转录组

Abstract:

Salt stress is one of the most important abiotic stresses affecting rice production. In this study,the transcriptome data of rice under salt stress were used to analyze the expression changes of transcription factors at the transcriptome level by HTSeq and DESeq software. The main results were as follows:there were 26 identical transcription factors differentially expressed in rice at 1,3 and 6 h of salt stress,and an important module containing 14 genes was screened by protein interaction. GO analysis of important modules showed that 14 genes were mainly enriched in ATP binding,stress response and cytoplasmic membrane-bounded vesicle. KEGG analysis showed that it was mainly enriched in endoplasmic reticulum protein processing and endocytosis pathway. Meanwhile,the key heat shock transcription factor gene Os03g074500 and Os06g0553100 induced by salt stress were identified. It is speculated that these two genes may play a significant role in the process of rice salt stress by regulating the transcription and expression of HSP70 gene and affecting protein folding and assembly. This study provides candidate genes for subsequent genetic improvement of rice and provides scientific enlightenment for further understanding of salt tolerance mechanism.

Key words: rice, key transcription factors, salt stress, protein interaction, transcriptome