介孔二氧化硅纳米粒在农业中的应用

doi:10.13560/j.cnki.biotech.bull.1985.2021-0731

摘要/Abstract

摘要：

介孔二氧化硅纳米粒（mesoporous silica nanoparticles,MSNs）具有高容量介孔空间、比表面积大、稳定性强、内外表面易于修饰和生物相容性好等优点,其在农业上的应用已成为当前国内外研究的热点。综述近年来国内外MSNs在农业生产上的应用进展,详细阐述MSNs作为载运系统在农业投入品和转基因方面的优势,讨论MSNs在环境污染治理、农产品贮藏保鲜、探测传感器等方面应用的特点,解析植物吸收、运输和积累MSNs的过程以及其与植物互作的原理,综合评估MSNs的生物安全性。最后,针对目前存在的问题和发展前景进行展望,以期为更有效地利用MSNs解决农业生产中的实际问题提供参考与理论支撑。

关键词: 介孔二氧化硅纳米粒, 载运体系, 生物安全性, 农业应用

Abstract:

Research of mesoporous silica nanoparticles（MSNs）for the application in agriculture has gained worldwide interest due to its unique properties such as high pore volume,large surface area,high stability,biocompatibility and facile surface functionalization. In this paper,the recent research progress of MSNs for agriculture is reviewed. The advantages of MSNs used as delivery system for agricultural inputs and transgene are well documented. The uniqueness of MSNs for environmental pollution control,agricultural product preservation and detection sensor is discussed. The uptake,transportation and accumulation of MSNs inside plants and the interaction between them are well explained,and the biosafe of MSNs is comprehensively evaluated. Finally,the future opportunities and challenges on MSNs for agriculture are prospected. This paper is aimed to provide reference and theoretical support for the further application of MSNs to effectively solve practical problems in agricultural production.

Key words: mesoporous silica nanoparticles, delivery systems, biosafe, agricultural application

孙德权, 陆新华, 李伟明, 胡玉林, 段雅婕, 庞振才, 胡会刚. 介孔二氧化硅纳米粒在农业中的应用[J]. 生物技术通报, 2022, 38(5): 228-239.

SUN De-quan, LU Xin-hua, LI Wei-ming, HU Yu-lin, DUAN Ya-jie, PANG Zhen-cai, HU Hui-gang. Application of Mesoporous Silica Nanoparticles in Agriculture[J]. Biotechnology Bulletin, 2022, 38(5): 228-239.

图/表 2

参考文献 91

[1]	陈星濛. 纳米技术在农业中的应用[J]. 湖北农业科学, 2019, 58(S2):13-15, 20.
	Chen XM. Application of nanotechnology in agriculture[J]. Hubei Agric Sci, 2019, 58(S2):13-15, 20.
[2]	Xin XP, Judy JD, Sumerlin BB, et al. Nano-enabled agriculture:from nanoparticles to smart nanodelivery systems[J]. Environ Chem, 2020, 17(6):413. doi: 10.1071/EN19254 URL
[3]	高鸿飞, 范世航, 刘婧琳, 等. 纳米材料在植物遗传转化中的应用[J]. 中国油料作物学报, 2021, 43(1):64-69.
	Gao HF, Fan SH, Liu JL, et al. Application of nanomaterials in plant genetic transformation[J]. Chin J Oil Crop Sci, 2021, 43(1):64-69.
[4]	Fu L, Wang Z, Dhankher OP, et al. Nanotechnology as a new sustainable approach for controlling crop diseases and increasing agricultural production[J]. J Exp Bot, 2020, 71(2):507-519. doi: 10.1093/jxb/erz314 URL
[5]	高梦迪, 盛茂银, 傅籍锋. 纳米材料对植物生长发育的影响[J]. 生物技术通报, 2019, 35(7):172-180. doi: 10.13560/j.cnki.biotech.bull.1985.2018-1022
	Gao MD, Sheng MY, Fu JF. Effects of nanomaterials on plant growth and development[J]. Biotechnol Bull, 2019, 35(7):172-180.
[6]	Guo HY, White JC, Wang ZY, et al. Nano-enabled fertilizers to control the release and use efficiency of nutrients[J]. Curr Opin Environ Sci Heal, 2018, 6:77-83.
[7]	Nuruzzaman M, Rahman MM, Liu Y, et al. Nanoencapsulation, nano-guard for pesticides:a new window for safe application[J]. J Agric Food Chem, 2016, 64(7):1447-1483. doi: 10.1021/acs.jafc.5b05214 URL
[8]	Sarlak N, Taherifar A, Salehi F. Synthesis of nanopesticides by encapsulating pesticide nanoparticles using functionalized carbon nanotubes and application of new nanocomposite for plant disease treatment[J]. J Agric Food Chem, 2014, 62(21):4833-4838. doi: 10.1021/jf404720d URL
[9]	Ribes À, Santiago-Felipe S, Bernardos A, et al. Two new fluorogenic aptasensors based on capped mesoporous silica nanoparticles to detect Ochratoxin A[J]. ChemistryOpen, 2017, 6(5):653-659. doi: 10.1002/open.201700106 URL
[10]	谭靓, 胡长鹰, 王志伟. 纳米二氧化钛抗菌食品包装复合膜的研究进展[J]. 食品安全质量检测学报, 2020, 11(22):8341-8350.
	Tan L, Hu CY, Wang ZW. Research progress of nano-titanium dioxide in antimicrobial food packaging composite film[J]. J Food Saf Qual, 2020, 11(22):8341-8350.
[11]	Popat A, Liu J, Hu QH, et al. Adsorption and release of biocides with mesoporous silica nanoparticles[J]. Nanoscale, 2012, 4(3):970-975. doi: 10.1039/C2NR11691J URL
[12]	Abdelrahman TM, Qin XY, Li DL, et al. Pectinase-responsive carriers based on mesoporous silica nanoparticles for improving the translocation and fungicidal activity of prochloraz in rice plants[J]. Chem Eng J, 2021, 404:126440. doi: 10.1016/j.cej.2020.126440 URL
[13]	刘强. 不同植物篱系统对坡耕地农田径流污染物的去除研究[D]. 雅安: 四川农业大学, 2016.
	Liu Q. Research on removal efficiency of the sloping farmland pollutants in surface runoff using different hedgerow systems[D]. Ya’an: Sichuan Agricultural University, 2016.
[14]	何顺, 高云昊, 万虎, 等. 基于介孔二氧化硅纳米粒子的农药可控释放研究进展[J]. 农药学学报, 2016, 18(4):416-423.
	He S, Gao YH, Wan H, et al. Recent progress in the application of mesoporous silica nanoparticles to controlled pesticides delivery system[J]. Chin J Pestic Sci, 2016, 18(4):416-423.
[15]	Jin L, Liu X, Bian CH, et al. Fabrication linalool-functionalized hollow mesoporous silica spheres nanoparticles for efficiently enhance bactericidal activity[J]. Chin Chem Lett, 2020, 31(8):2137-2141. doi: 10.1016/j.cclet.2019.12.020 URL
[16]	Li ZZ, Wen LX, Shao L, et al. Fabrication of porous hollow silica nanoparticles and their applications in drug release control[J]. J Control Release, 2004, 98(2):245-254. doi: 10.1016/j.jconrel.2004.04.019 URL
[17]	Wen LX, Li ZZ, Zou HK, et al. Controlled release of avermectin from porous hollow silica nanoparticles[J]. Pest Manag Sci, 2005, 61(6):583-590. pmid: 15714463
[18]	Liu F, Wen LX, Li ZZ, et al. Porous hollow silica nanoparticles as controlled delivery system for water-soluble pesticide[J]. Mater Res Bull, 2006, 41(12):2268-2275. doi: 10.1016/j.materresbull.2006.04.014 URL
[19]	Li ZZ, Xu SA, Wen LX, et al. Controlled release of avermectin from porous hollow silica nanoparticles:influence of shell thickness on loading efficiency, UV-shielding property and release[J]. J Control Release, 2006, 111(1/2):81-88. doi: 10.1016/j.jconrel.2005.10.020 URL
[20]	Li ZZ, Chen JF, Liu F, et al. Study of UV-shielding properties of novel porous hollow silica nanoparticle carriers for avermectin[J]. Pest Manag Sci, 2007, 63(3):241-246. doi: 10.1002/ps.1301 URL
[21]	Sharma MVP, Sadanandam G, Ratnamala A, et al. An efficient and novel porous nanosilica supported TiO₂ photocatalyst for pesticide degradation using solar light[J]. J Hazard Mater, 2009, 171(1/2/3):626-633. doi: 10.1016/j.jhazmat.2009.06.040 URL
[22]	Prado AGS, Moura AO, Nunes AR. Nanosized silica modified with carboxylic acid as support for controlled release of herbicides[J]. J Agric Food Chem, 2011, 59(16):8847-8852. doi: 10.1021/jf202509g URL
[23]	Chen J, Wang W, Xu Y, et al. Slow-release formulation of a new biological pesticide, pyoluteorin, with mesoporous silica[J]. J Agric Food Chem, 2011, 59(1):307-311. doi: 10.1021/jf103640t URL
[24]	Wibowo D, Zhao CX, Peters BC, et al. Sustained release of fipronil insecticide in vitro and in vivo from biocompatible silica nanocapsules[J]. J Agric Food Chem, 2014, 62(52):12504-12511. doi: 10.1021/jf504455x URL
[25]	Wanyika H. Sustained release of fungicide metalaxyl by mesoporous silica nanospheres[J]. J Nanoparticle Res, 2013, 15(8):1-9.
[26]	Janatova A, Bernardos A, Smid J, et al. Long-term antifungal activity of volatile essential oil components released from mesoporous silica materials[J]. Ind Crops Prod, 2015, 67:216-220. doi: 10.1016/j.indcrop.2015.01.019 URL
[27]	Chen HY, Lin YS, Zhou HJ, et al. Synthesis and characterization of chlorpyrifos/copper(II)schiff base mesoporous silica with pH sensitivity for pesticide sustained release[J]. J Agric Food Chem, 2016, 64(43):8095-8102. doi: 10.1021/acs.jafc.6b03262 URL
[28]	Chen HY, Lin YS, Zhou HJ, et al. Highly efficient alginate sodium encapsulated chlorpyrifos/copper(ii)Schiff base mesoporous silica sustained release system with pH and ion response for pesticide delivery[J]. RSC Adv, 2016, 6(115):114714-114721. doi: 10.1039/C6RA23836J URL
[29]	Cao LD, Zhang HR, Cao C, et al. Quaternized chitosan-capped mesoporous silica nanoparticles as nanocarriers for controlled pesticide release[J]. Nanomaterials, 2016, 6(7):126. doi: 10.3390/nano6070126 URL
[30]	Sun RJ, Wang WQ, Wen YQ, et al. Recent advance on mesoporous silica nanoparticles-based controlled release system:intelligent switches open up new horizon[J]. Nanomaterials, 2015, 5(4):2019-2053. doi: 10.3390/nano5042019 URL
[31]	Kaziem AE, Gao YH, He S, et al. Synthesis and insecticidal activity of enzyme-triggered functionalized hollow mesoporous silica for controlled release[J]. J Agric Food Chem, 2017, 65(36):7854-7864. doi: 10.1021/acs.jafc.7b02560 URL
[32]	Kaziem AE, Gao Y, Zhang Y, et al. Α-Amylase triggered carriers based on cyclodextrin anchored hollow mesoporous silica for enhancing insecticidal activity of avermectin against Plutella xylostella[J]. J Hazard Mater, 2018, 359:213-221. doi: S0304-3894(18)30589-2 pmid: 30036751
[33]	Xu CL, Cao LD, Zhao PY, et al. Emulsion-based synchronous pesticide encapsulation and surface modification of mesoporous silica nanoparticles with carboxymethyl chitosan for controlled azoxystrobin release[J]. Chem Eng J, 2018, 348:244-254. doi: 10.1016/j.cej.2018.05.008 URL
[34]	Lu X, Sun D, Rookes JE, et al. Nanoapplication of a resistance inducer to reduce Phytophthora disease in pineapple(Ananas comosus L.)[J]. Front Plant Sci, 2019, 10:1238. doi: 10.3389/fpls.2019.01238 URL
[35]	Shan YP, Cao LD, Xu CL, et al. Sulfonate-functionalized mesoporous silica nanoparticles as carriers for controlled herbicide diquat dibromide release through electrostatic interaction[J]. Int J Mol Sci, 2019, 20(6):1330. doi: 10.3390/ijms20061330 URL
[36]	Gao YH, Xiao YN, Mao KK, et al. Thermoresponsive polymer-encapsulated hollow mesoporous silica nanoparticles and their application in insecticide delivery[J]. Chem Eng J, 2020, 383:123169. doi: 10.1016/j.cej.2019.123169 URL
[37]	Zhao PY, Cao LD, Ma DK, et al. Synthesis of pyrimethanil-loaded mesoporous silica nanoparticles and its distribution and dissipation in cucumber plants[J]. Molecules, 2017, 22(5):817. doi: 10.3390/molecules22050817 URL
[38]	Zhao P, Cao L, Ma D, et al. Translocation, distribution and degradation of prochloraz-loaded mesoporous silica nanoparticles in cucumber plants[J]. Nanoscale, 2018, 10(4):1798-1806. doi: 10.1039/C7NR08107C URL
[39]	Xu YB, Xu CL, Huang QL, et al. Size effect of mesoporous silica nanoparticles on pesticide loading, release, and delivery in cucumber plants[J]. Appl Sci, 2021, 11(2):575. doi: 10.3390/app11020575 URL
[40]	Zhu F, Liu XG, Cao LD, et al. Uptake and distribution of fenoxanil-loaded mesoporous silica nanoparticles in rice plants[J]. Int J Mol Sci, 2018, 19(10):2854. doi: 10.3390/ijms19102854 URL
[41]	Sattary M, Amini J, Hallaj R. Antifungal activity of the lemongrass and clove oil encapsulated in mesoporous silica nanoparticles against wheat’s take-all disease[J]. Pestic Biochem Physiol, 2020, 170:104696. doi: S0048-3575(20)30191-7 pmid: 32980050
[42]	汪玉洁, 陈日远, 刘厚诚, 等. 纳米材料在农业上的应用及其对植物生长和发育的影响[J]. 植物生理学报, 2017, 53(6):933-942.
	Wang YJ, Chen RY, Liu HC, et al. Applications of nanomaterials in agriculture and its effects on the growth and development of plants[J]. Plant Physiol J, 2017, 53(6):933-942.
[43]	Wanyika H, Gatebe E, Kioni P, et al. Mesoporous silica nanoparticles carrier for urea:potential applications in agrochemical delivery systems[J]. J Nanosci Nanotechnol, 2012, 12(3):2221-2228. doi: 10.1166/jnn.2012.5801 URL
[44]	de Silva M, Siriwardena DP, Sandaruwan C, et al. Urea-silica nanohybrids with potential applications for slow and precise release of nitrogen[J]. Mater Lett, 2020, 272:127839. doi: 10.1016/j.matlet.2020.127839 URL
[45]	Singh K, Ram S, Nehra A, et al. Effect of magnetized water on urea-loading efficiency of mesoporous nano-silica:a seed germination study on wheat crop[J]. J Nanosci Nanotechnol, 2019, 19(4):2016-2026. doi: 10.1166/jnn.2019.16508 URL
[46]	白杨. 纳米SiO₂-聚乙烯醇-γ-聚谷氨酸复合物包膜肥料研制及其养分缓释机理[D]. 沈阳: 沈阳农业大学, 2020.
	Bai Y. Manufacture of nano-SiO₂-polyvinyl alcohol-γ-glutamic acid composite coated fertilizer and its nutrient slow and release mechanism[D]. Shenyang: Shenyang Agricultural University, 2020.
[47]	Mushtaq A, Jamil N, Rizwan S, et al. Engineered Silica Nanoparticles and silica nanoparticles containing controlled release fertilizer for drought and saline areas[J]. IOP Conf Ser:Mater Sci Eng, 2018, 414:012029. doi: 10.1088/1757-899X/414/1/012029 URL
[48]	Naseem F, Zhi Y, Farrukh MA, et al. Mesoporous ZnAl₂Si₁₀O₂₄ nanofertilizers enable high yield of Oryza sativa L[J]. Sci Rep, 2020, 10:10841. doi: 10.1038/s41598-020-67611-4 pmid: 32616915
[49]	孙德权, 陆新华, 陈海丽, 等. 介孔二氧化硅纳米肥料的制备及控制释放[J]. 热带作物学报, 2020, 41(9):1905-1911.
	Sun DQ, Lu XH, Chen HL, et al. Preparation and controlled release of nanofertilizer mediated by mesoporous silica nanoparticles[J]. Chin J Trop Crops, 2020, 41(9):1905-1911.
[50]	Hajiahmadi Z, Shirzadian-Khorramabad R, Kazemzad M, et al. A novel, simple, and stable mesoporous silica nanoparticle-based gene transformation approach in Solanum lycopersicum[J]. 3 Biotech, 2020, 10(8):370. doi: 10.1007/s13205-020-02359-2 pmid: 32832330
[51]	Torney F, Trewyn BG, Lin VS, et al. Mesoporous silica nanoparticles deliver DNA and chemicals into plants[J]. Nat Nanotechnol, 2007, 2(5):295-300. doi: 10.1038/nnano.2007.108 URL
[52]	Martin-Ortigosa S, Valenstein JS, Lin VSY, et al. Gold functionalized mesoporous silica nanoparticle mediated protein and DNA codelivery to plant cells via the biolistic method[J]. Adv Funct Mater, 2012, 22(17):3576-3582. doi: 10.1002/adfm.201200359 URL
[53]	Martin-Ortigosa S, Peterson DJ, Valenstein JS, et al. Mesoporous silica nanoparticle-mediated intracellular cre protein delivery for maize genome editing via loxP site excision[J]. Plant Physiol, 2014, 164(2):537-547. doi: 10.1104/pp.113.233650 pmid: 24376280
[54]	Zolghadrnasab M, Mousavi A, Farmany A, et al. Ultrasound-mediated gene delivery into suspended plant cells using polyethyleneimine-coated mesoporous silica nanoparticles[J]. Ultrason Sonochem, 2021, 73:105507. doi: 10.1016/j.ultsonch.2021.105507 URL
[55]	Chang FP, Kuang LY, Huang CA, et al. A simple plant gene delivery system using mesoporous silica nanoparticles as carriers[J]. J Mater Chem B, 2013, 1(39):5279-5287. doi: 10.1039/c3tb20529k URL
[56]	Fu YQ, Li LH, Wang H, et al. Silica nanoparticles-mediated stable genetic transformation in Nicotiana tabacum[J]. Chem Res Chin Univ, 2015, 31(6):976-981. doi: 10.1007/s40242-015-5088-0 URL
[57]	Yang H, Zheng K, Zhang ZM, et al. Adsorption and protection of plasmid DNA on mesoporous silica nanoparticles modified with various amounts of organosilane[J]. J Colloid Interface Sci, 2012, 369(1):317-322. doi: 10.1016/j.jcis.2011.12.043 URL
[58]	Niu BY, Zhou YX, Wen T, et al. Proper functional modification and optimized adsorption conditions improved the DNA loading capacity of mesoporous silica nanoparticles[J]. Colloids Surf A:Physicochem Eng Aspects, 2018, 548:98-107. doi: 10.1016/j.colsurfa.2018.03.035 URL
[59]	Xie Y, Cheng W, Tsang PE, et al. Remediation and phytotoxicity of decabromodiphenyl ether contaminated soil by zero valent iron nanoparticles immobilized in mesoporous silica microspheres[J]. J Environ Manage, 2016, 166:478-483. doi: 10.1016/j.jenvman.2015.10.042 URL
[60]	Fouad DM, El-Said WA, Ali MH, et al. Silica-gold nanocomposite for removal of organophosphorous pesticides[J]. Plasmonics, 2017, 12(3):869-875. doi: 10.1007/s11468-016-0338-7 URL
[61]	Albertini F, Ribeiro T, Alves S, et al. Boron-chelating membranes based in hybrid mesoporous silica nanoparticles for water purification[J]. Mater Des, 2018, 141:407-413. doi: 10.1016/j.matdes.2018.01.001 URL
[62]	Mohamad DF, Osman NS, Nazri MKHM, et al. Synthesis of mesoporous silica nanoparticle from banana peel ash for removal of phenol and methyl orange in aqueous solution[J]. Mater Today:Proc, 2019, 19:1119-1125.
[63]	Beagan AM. Cholesterol-functionalised mesoporous silica nanoparticles for removal of naphthalene from water[J]. Int J Environ Anal Chem, 2021:1-15.
[64]	To PK, Ma HT, Nguyen Hoang L, et al. Nitrate removal from waste-water using silica nanoparticles[J]. J Chem, 2020, 2020:1-6.
[65]	Kenawy IMM, Abou El-Reash YG, Hassanien MM, et al. Use of microwave irradiation for modification of mesoporous silica nanoparticles by thioglycolic acid for removal of cadmium and mercury[J]. Microporous Mesoporous Mater, 2018, 258:217-227. doi: 10.1016/j.micromeso.2017.09.021 URL
[66]	Al-Asmar A, Giosafatto CVL, Sabbah M, et al. Effect of mesoporous silica nanoparticles on the physicochemical properties of pectin packaging material for strawberry wrapping[J]. Nanomaterials, 2019, 10(1):52. doi: 10.3390/nano10010052 URL
[67]	Shi SY, Wang W, Liu LQ, et al. Effect of chitosan/nano-silica coating on the physicochemical characteristics of longan fruit under ambient temperature[J]. J Food Eng, 2013, 118(1):125-131. doi: 10.1016/j.jfoodeng.2013.03.029 URL
[68]	尹月玲, 刘瑶, 章建浩, 等. 纳米SiO₂复合涂膜材料包装松花蛋的保鲜效果[J]. 农业工程学报, 2012, 28(S1):281-287.
	Yin YL, Liu Y, Zhang JH, et al. Effect of composite conting with nano-silicon dioxide on fresh-keeping of preserved eggs[J]. Trans Chin Soc Agric Eng, 2012, 28(S1):281-287.
[69]	Tan H, Ma L, Guo T, et al. A novel fluorescence aptasensor based on mesoporous silica nanoparticles for selective and sensitive detection of aflatoxin B₁[J]. Anal Chim Acta, 2019, 1068:87-95. doi: 10.1016/j.aca.2019.04.014 URL
[70]	Xu Y, Kutsanedzie FYH, Hassan M, et al. Mesoporous silica supported orderly-spaced gold nanoparticles SERS-based sensor for pesticides detection in food[J]. Food Chem, 2020, 315:126300. doi: 10.1016/j.foodchem.2020.126300 URL
[71]	Sánchez G, Curiel D, Ratera I, et al. Modified mesoporous silica nanoparticles as a reusable, selective chromogenic sensor for mercury(II)recognition[J]. Dalton Trans, 2013, 42(18):6318-6326. doi: 10.1039/c2dt32243a pmid: 23325035
[72]	Nair R, Varghese SH, Nair BG, et al. Nanoparticulate material delivery to plants[J]. Plant Sci, 2010, 179(3):154-163. doi: 10.1016/j.plantsci.2010.04.012 URL
[73]	Lee H, Lytton-Jean AK, Chen Y, et al. Molecularly self-assembled nucleic acid nanoparticles for targeted in vivo siRNA delivery[J]. Nat Nanotechnol, 2012, 7(6):389-393. doi: 10.1038/nnano.2012.73 URL
[74]	Hussain HI, Yi ZF, Rookes JE, et al. Mesoporous silica nanoparticles as a biomolecule delivery vehicle in plants[J]. J Nanoparticle Res, 2013, 15(6):1-15.
[75]	Sun D, Hussain HI, Yi Z, et al. Uptake and cellular distribution, in four plant species, of fluorescently labeled mesoporous silica nanoparticles[J]. Plant Cell Rep, 2014, 33(8):1389-1402. doi: 10.1007/s00299-014-1624-5 URL
[76]	Sun D, Hussain HI, Yi Z, et al. Mesoporous silica nanoparticles enhance seedling growth and photosynthesis in wheat and lupin[J]. Chemosphere, 2016, 152:81-91. doi: 10.1016/j.chemosphere.2016.02.096 URL
[77]	Zhao P, Yuan W, Xu C, et al. Enhancement of spirotetramat transfer in cucumber plant using mesoporous silica nanoparticles as carriers[J]. J Agric Food Chem, 2018, 66(44):11592-11600. doi: 10.1021/acs.jafc.8b04415 URL
[78]	李博, 陶功胜, 谢寅峰, 等. 叶面喷施纳米SiO₂对髯毛箬竹的生理调节效应[J]. 南京林业大学学报:自然科学版, 2012, 36(4):161-164.
	Li B, Tao GS, Xie YF, et al. Physiological effects under the condition of spraying nano-SiO₂ onto the Indocalamus barbatus McClure leaves[J]. J Nanjing For Univ:Nat Sci Ed, 2012, 36(4):161-164.
[79]	Suriyaprabha R, Karunakaran G, Yuvakkumar R, et al. Silica nanoparticles for increased silica availability in maize(Zea mays. L)seeds under hydroponic conditions[J]. Curr Nanosci, 2012, 8(6):902-908. doi: 10.2174/157341312803989033 URL
[80]	Tantawy AS, Salama YM, El-Nemr MA, et al. Nano silicon application improves salinity tolerance of sweet pepper plants[J]. Int J Chem Res Technol, 2015, 8(10):11-17.
[81]	Suciaty T, Purnomo D, Sakya AT, et al. The effect of nano-silica fertilizer concentration and rice hull ash doses on soybean(Glycine max(L.)Merrill)growth and yield[J]. IOP Conf Ser:Earth Environ Sci, 2018, 129:012009. doi: 10.1088/1755-1315/129/1/012009 URL
[82]	Cui J, Liu T, Li F, et al. Silica nanoparticles alleviate cadmium toxicity in rice cells:Mechanisms and size effects[J]. Environ Pollut, 2017, 228:363-369. doi: 10.1016/j.envpol.2017.05.014 URL
[83]	de Asgari F, Majd A, Jonoubi P, et al. Effects of silicon nanoparticles on molecular, chemical, structural and ultrastructural characteristics of oat(Avena sativa L.)[J]. Plant Physiol Biochem, 2018, 127:152-160. doi: 10.1016/j.plaphy.2018.03.021 URL
[84]	Le VN, Rui Y, Gui X, et al. Uptake, transport, distribution and bio-effects of SiO₂ nanoparticles in Bt-transgenic cotton[J]. J Nanobiotechnology, 2014, 12:50. doi: 10.1186/s12951-014-0050-8 URL
[85]	Derbalah A, Shenashen M, Hamza A, et al. Antifungal activity of fabricated mesoporous silica nanoparticles against early blight of tomato[J]. Egypt J Basic Appl Sci, 2018, 5(2):145-150.
[86]	Buchman JT, Elmer WH, Ma CX, et al. Chitosan-coated mesoporous silica nanoparticle treatment of Citrullus lanatus(watermelon):enhanced fungal disease suppression and modulated expression of stress-related genes[J]. ACS Sustainable Chem Eng, 2019, 7(24):19649-19659. doi: 10.1021/acssuschemeng.9b04800 URL
[87]	Lu X, Sun D, Zhang X, et al. Stimulation of photosynthesis and enhancement of growth and yield in Arabidopsis thaliana treated with amine-functionalized mesoporous silica nanoparticles[J]. Plant Physiol Biochem, 2020, 156:566-577. doi: 10.1016/j.plaphy.2020.09.036 URL
[88]	龚束芳, 刘阳, 速馨逸, 等. 纳米硅肥对远东芨芨草幼苗模拟抗旱的影响[J]. 草业科学, 2018, 35(12):2924-2930.
	Gong SF, Liu Y, Su XY, et al. Influence of nano-silicon fertilizer on osmotic stress in Achnatherum extremiorientale[J]. Pratacultural Sci, 2018, 35(12):2924-2930.
[89]	刘俊渤, 常海波, 马景勇, 等. 纳米SiO₂对水稻稻瘟病的抗病效应及对水稻生长发育的影响[J]. 吉林农业大学学报, 2012, 34(2):157-161, 165.
	Liu JB, Chang HB, Ma JY, et al. Effects of nano-silica on rice’s resistance to Magnaporthe oryzae and on rice growth[J]. J Jilin Agric Univ, 2012, 34(2):157-161, 165.
[90]	王荔军, 王运华, 周益林, 等. 纳米结构SiO₂与植物真菌病害发生的关系[J]. 华中农业大学学报, 2001, 20(6):593-597.
	Wang LJ, Wang YH, Zhou YL, et al. Relationship between nanostructure SiO₂ and occurrence of plant fungi[J]. J Huazhong Agric, 2001, 20(6):593-597.
[91]	Khodakovskaya MV, de Silva K, Nedosekin DA, et al. Complex genetic, photothermal, and photoacoustic analysis of nanoparticle-plant interactions[J]. PNAS, 2011, 108(3):1028-1033. doi: 10.1073/pnas.1008856108 pmid: 21189303