生物技术通报 ›› 2022, Vol. 38 ›› Issue (5): 93-99.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0937

• 研究报告 • 上一篇    下一篇

花生疮痂病菌蓝光受体EaWC 1基因克隆及生物信息学分析

李洋(), 张晓天, 朴静子, 周如军(), 李自博, 关海雯   

  1. 沈阳农业大学植物保护学院,沈阳 110866
  • 收稿日期:2021-07-22 出版日期:2022-05-26 发布日期:2022-06-10
  • 作者简介:李洋,女,硕士研究生,研究方向:植物病原真菌学;E-mail: liyang20210713@163.com
  • 基金资助:
    辽宁省自然科学基金项目(2019-MS-278)

Cloning and Bioinformatics Analysis of Blue-light Receptor EaWC 1 Gene in Elsinoë arachidis

LI Yang(), ZHANG Xiao-tian, PIAO Jing-zi, ZHOU Ru-jun(), LI Zi-bo, GUAN Hai-wen   

  1. College of Plant Protection,Shenyang Agricultural University,Shenyang 110866
  • Received:2021-07-22 Published:2022-05-26 Online:2022-06-10

摘要:

光是真菌感知和适应环境的重要信息载体,调控多种生理生化过程。痂囊腔菌素(Elsinochromes,ESC)是花生疮痂病菌重要的毒力因子,蓝光对其具有正调控作用,为了进一步揭示光调控ESC机制,开展了花生疮痂病菌蓝光受体基因EaWC 1克隆、生物信息学分析和表达模式研究。结果表明,蓝光受体基因EaWC 1全长为3 261bp,具有一个完整的开放阅读框,编码长度为1 086个氨基酸的蛋白质,相对分子质量11.95 kD,理论等电点为8.81,具有核定位信号,为稳定亲水性蛋白。qPCR定量分析表明,EaWC 1基因表达受到蓝光诱导,其表达模式与ESC毒素含量呈显著正相关。研究结果对于阐明花生疮痂病菌蓝光受体生物功能,揭示ESC毒素生物合成光调控机制和调控网络奠定了理论基础。

关键词: Elsinoë arachidis, 蓝光受体, 基因克隆, 生物信息学分析, 表达分析

Abstract:

Light is an important information carrier for fungi to perceive and adapt to the environment factors,regulate a variety of physiological and biochemical processes. Elsinochromes(ESC)is an important virulence factor of Elsinoë arachidis causing peanut scab. In order to further reveal mechanism of light regulation on ESC production,gene cloning,bioinformatics analysis and expression of the blue-light receptor gene EaWC 1 were conducted. The results showed that the blue light receptor gene EaWC-1 had a complete reading frame(ORF)with 3 261 bp in length that encoded a protein of 1 086 amino acids. The molecular weight of the protein was 11.95 kD,the theoretical isoelectric point was 8.81. Subcellular localization analysis showed that EaWC-1 was localized in nucleus and was a hydrophilic protein. qPCR analysis indicated that the expression of EaWC 1 gene was positively regulated by blue light,and its expression pattern was significantly and positively correlated with ESC toxin accumulation. This result would provide a theoretical foundation for elucidating the biological functions of blue light receptors of E. arachidis and revealing the photo-regulation mechanism and regulatory network of ESC toxin biosynthesis.

Key words: Elsinoë arachidis, blue-light receptor, gene cloning, bioinformatics analysis, expression analysis