联合用药对嗜水气单胞菌耐药性影响研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2021-1004

摘要/Abstract

摘要：

嗜水气单胞菌（Aeromonas hydrophila）是威胁水产养殖业安全最严重的病原微生物之一,同时还存在人畜共患的典型特征。随着抗生素、抗菌药物的主动使用和被动暴露（医院生活废水、畜禽养殖污水排放至养殖水体环境）,嗜水气单胞菌多重耐药问题日益突出,给水产养殖和人体健康均带来潜在风险。而合理联合用药不仅可减缓和阻控细菌耐药性产生,还能减少药物残留。本文首先分析联合用药遵循的原则及应关注的方向,阐述耐药产生的原因及联合用药影响耐药性的作用机制,综述研究联合用药阻控嗜水气单胞菌耐药性的动态进展,最后提出如何推进的思考和展望,旨在为水产养殖业通过联合用药手段控制嗜水气单胞菌耐药性提供防控策略和解决手段。

关键词: 联合用药, 嗜水气单胞菌, 细菌耐药性, 研究进展

Abstract:

Aeromonas hydrophila is one of the most serious pathogenic microorganisms threatening the aquaculture industry,and it also has characteristic of zoonotic diseases. Due to the using of antibiotics or antibacterial drugs and exposure to hospitals wastewater,livestock and poultry farming wastewater,the problem of multi-drug resistance of A. hydrophila has become increasingly prominent,which contributes potential risks to the aquiculture safety and human being health. The rational joint medicationcan not only slow down and prevent the emergence of bacterial resistance,but also reduce drug residues. This paper first analyzes the principles followed by the joint medication and the direction to be paid attention to,expounds the causes of drug resistance and the mechanism of the effect of the joint medication on drug resistance,and synthesizes the dynamics progress of the joint medication to block drug resistance in A. hydrophila. Finally,the thoughts and prospects of how to proceed are proposed,aiming to provide prevention and control strategies and solutions for the aquaculture industry to control the drug resistance of A. hydrophila through joint medication.

Key words: joint medication, Aeromonas hydrophila, bacterial resistance, research progress

赵海晴, 李耘, 梁严内, 刘哲, 任亚林, 李金娟. 联合用药对嗜水气单胞菌耐药性影响研究进展[J]. 生物技术通报, 2022, 38(6): 53-65.

ZHAO Hai-qing, LI Yun, LIANG Yan-nei, LIU Zhe, REN Ya-lin, LI Jin-juan. Advances in Research on the Effects of Joint Medication on the Drug Resistance of Aeromonas hydrophila[J]. Biotechnology Bulletin, 2022, 38(6): 53-65.

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地区 Region	样本类型 Sample type	占气单胞菌比例 Proportion of Aeromonas/%	耐受药物种类 Types of resistant drug	时间 Year	毒力基因类型 Virulence gene	参考文献 References
中国	水样	3	β-内酰胺类;多粘菌素类;青霉素类	2020	act,aerA,alt,ascV,ast,ela,fla,hlyA,laf,lip	Meng et al. 2020^[9]
中国	胭脂鱼	\	大环内酯类;林可酰胺类;青霉素类;糖肽类	2019	aerA,aexT,alt,ascV,aspA,ast,hlyA,laA,lip,ompA	Li et al. 2019^[10]
埃及	鲻鱼	100	β-内酰胺类;青霉素类	2018	act,aerA,ast,hlyA	Ramadan et al. 2018^[11]
埃及	罗非鱼	35	氨基糖苷类;碳青霉烯类;四环素类	2018	act,aerA,alt,asa1	Hammad et al. 2018^[12]
意大利	淡水鱼	56	β-内酰胺类;氨基糖苷类;氯霉素类;青霉素类;大环内酯类;四环素类	2020	\	Borella et al. 2020^[13]
韩国	乌龟	32	β-内酰胺类;氯霉素类;青霉素类;四环素类;萘啶酸	2017	act,alt,ast	Wimalasena et al. 2017^[14]
	斑马鱼	19	β-内酰胺类;利福平;氯霉素类;萘啶酸;四环素类;碳青霉烯类	2018	act,aerA,ahyB,alt,ascV,ast,fla,gcat,hlyA,lip,ser	Hossain et al. 2018^[15]
	虾夷扇贝	41	β-内酰胺类;氨基糖苷类;大环内酯类;喹诺酮类;氯霉素类;青霉素类;四环素类	2019	act,aerA,ahyB,alt,ascV,ast,fla,gcat,hlyA,lip,ser	De Silva et al. 2019^[16]
	孔雀鱼	6	β-内酰胺类;氨基糖苷类;喹诺酮类;利福平;青霉素类;四环素类;碳青霉烯类	2020	act,aerA,ahyB,alt,ascV,ast,fla,gcat,hlyA,lip,ser	Hossain et al. 2020^[17]
墨西哥	冻鱼	3	β-内酰胺类;氨基糖苷类;大环内酯类;利福平;林可酰胺类;青霉素类;碳青霉烯类	2003	aerA,gcat,lip,ser,β-hemol	Castro-Escarpulli et al. 2003^[18]
太平洋	鲍鱼	31	β-内酰胺类;氨基糖苷类;多肽类;利福平;萘啶酸;青霉素类;四环素类	2020	aerA,act,ahyB,alt,ascV,ast,exu,fla,gcat,hlyA,lip,ser	Wickramanayake et al. 2020^[19]
葡萄牙	水样	26	β-内酰胺类;氨基糖苷类;大环内酯类;青霉素类;四环素类	2012	aerA,ascV,lip,prot	Carvalho et al. 2012^[20]
乌拉圭	水产动物	38	大环内酯类;氯霉素类;青霉素类;四环素类	2018	act,alt,ast,ela,lip	Perretta et al. 2018^[21]
美国	水样	22	四环素类;喹诺酮类	2014	aerA,lip,Nucl,ser	Skwor et al. 2014^[22]

地区 Region	样本类型 Sample type	占气单胞菌比例 Proportion of Aeromonas/%	耐受药物种类 Types of resistant drug	时间 Year	毒力基因类型 Virulence gene	参考文献 References
中国	水样	3	β-内酰胺类;多粘菌素类;青霉素类	2020	act,aerA,alt,ascV,ast,ela,fla,hlyA,laf,lip	Meng et al. 2020^[9]
中国	胭脂鱼	\	大环内酯类;林可酰胺类;青霉素类;糖肽类	2019	aerA,aexT,alt,ascV,aspA,ast,hlyA,laA,lip,ompA	Li et al. 2019^[10]
埃及	鲻鱼	100	β-内酰胺类;青霉素类	2018	act,aerA,ast,hlyA	Ramadan et al. 2018^[11]
埃及	罗非鱼	35	氨基糖苷类;碳青霉烯类;四环素类	2018	act,aerA,alt,asa1	Hammad et al. 2018^[12]
意大利	淡水鱼	56	β-内酰胺类;氨基糖苷类;氯霉素类;青霉素类;大环内酯类;四环素类	2020	\	Borella et al. 2020^[13]
韩国	乌龟	32	β-内酰胺类;氯霉素类;青霉素类;四环素类;萘啶酸	2017	act,alt,ast	Wimalasena et al. 2017^[14]
	斑马鱼	19	β-内酰胺类;利福平;氯霉素类;萘啶酸;四环素类;碳青霉烯类	2018	act,aerA,ahyB,alt,ascV,ast,fla,gcat,hlyA,lip,ser	Hossain et al. 2018^[15]
	虾夷扇贝	41	β-内酰胺类;氨基糖苷类;大环内酯类;喹诺酮类;氯霉素类;青霉素类;四环素类	2019	act,aerA,ahyB,alt,ascV,ast,fla,gcat,hlyA,lip,ser	De Silva et al. 2019^[16]
	孔雀鱼	6	β-内酰胺类;氨基糖苷类;喹诺酮类;利福平;青霉素类;四环素类;碳青霉烯类	2020	act,aerA,ahyB,alt,ascV,ast,fla,gcat,hlyA,lip,ser	Hossain et al. 2020^[17]
墨西哥	冻鱼	3	β-内酰胺类;氨基糖苷类;大环内酯类;利福平;林可酰胺类;青霉素类;碳青霉烯类	2003	aerA,gcat,lip,ser,β-hemol	Castro-Escarpulli et al. 2003^[18]
太平洋	鲍鱼	31	β-内酰胺类;氨基糖苷类;多肽类;利福平;萘啶酸;青霉素类;四环素类	2020	aerA,act,ahyB,alt,ascV,ast,exu,fla,gcat,hlyA,lip,ser	Wickramanayake et al. 2020^[19]
葡萄牙	水样	26	β-内酰胺类;氨基糖苷类;大环内酯类;青霉素类;四环素类	2012	aerA,ascV,lip,prot	Carvalho et al. 2012^[20]
乌拉圭	水产动物	38	大环内酯类;氯霉素类;青霉素类;四环素类	2018	act,alt,ast,ela,lip	Perretta et al. 2018^[21]
美国	水样	22	四环素类;喹诺酮类	2014	aerA,lip,Nucl,ser	Skwor et al. 2014^[22]