生物技术通报 ›› 2023, Vol. 39 ›› Issue (10): 219-230.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0071

• 研究报告 • 上一篇    下一篇

菠萝不同品种对低温胁迫响应差异的生理代谢机制

刘传和1(), 贺涵1, 何秀古2(), 陈鑫1, 刘开1, 邵雪花1, 赖多1, 秦健1, 庄庆礼1, 匡石滋1, 肖维强1   

  1. 1.广东省农业科学院果树研究所 农业农村部南亚热带果树生物学与遗传资源利用重点实验室 广东省热带亚热带果树研究重点实验室,广州 510640
    2.广东省农业科学院,广州 510640
  • 收稿日期:2023-02-01 出版日期:2023-10-26 发布日期:2023-11-28
  • 通讯作者: 何秀古,男,研究员,研究方向:菠萝优质高效种植技术研究与应用推广;E-mail: hexiugu@gdaas.cn
  • 作者简介:刘传和,男,博士,研究员,研究方向:菠萝优质高效种植技术与新品种选育;E-mail: founderlch@126.com
  • 基金资助:
    乡村振兴战略专项资金(403-2018-XMZC-0002-90);乡村振兴战略专项资金(TS-3-1);国家重点研发计划项目(2019YFD1001100);广东省现代农业产业技术体系创新团队建设专项资金(2023KJ109);广东省农业科学院菠萝产业研究院项目,广东省农村科技特派员项目(KTP20210395);广州市科技计划项目(2023E04J0562)

Physiological and Metabolitic Mechanisms of Different Pineapple Cultivars Responding to Low Temperature Stress

LIU Chuan-he1(), HE Han1, HE Xiu-gu2(), CHEN Xin1, LIU Kai1, SHAO Xue-hua1, LAI Duo1, QIN Jian1, ZHUANG Qing-li1, KUANG Shi-zi1, XIAO Wei-qiang1   

  1. 1. Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangzhou 510640
    2. Guangdong Academy of Agricultural Sciences, Guangzhou 510640
  • Received:2023-02-01 Published:2023-10-26 Online:2023-11-28

摘要:

为探明菠萝不同品种对低温胁迫响应差异的相关机理,本研究以耐寒性强的‘粤甜’和耐寒性弱的‘巴厘’品种为试材,对常温(25℃)及低温(5℃)处理48 h植株的叶片生理指标、基因相对表达量及差异代谢物等进行比较测定。结果表明,低温处理后‘粤甜’的可溶性蛋白、可溶性糖、脯氨酸含量及POD、SOD、CAT酶活性均显著高于‘巴厘’,丙二醛含量显著低于‘巴厘’;PODCAT基因相对表达量显著高于‘巴厘’,ProDH基因显著低于‘巴厘’;内源生长素、细胞分裂素、茉莉酸、水杨酸及独角金内酯含量高于‘巴厘’,赤霉素、脱落酸含量低于‘巴厘’。代谢组分析表明,低温处理后‘粤甜’与‘巴厘’间共检测出差异代谢物262个,其中黄酮类代谢物最多。KEGG分析表明‘粤甜’‘巴厘’间差异代谢物主要富集在次生代谢物生物合成、类黄酮生物合成、黄酮与黄酮醇生物合成等代谢通路中。筛选出110个低温诱导的‘粤甜’与‘巴厘’差异代谢物,其中41个在常温下差异显著、低温处理后差异倍数增大,69个在常温下无显著差异、低温处理后差异显著;差异代谢物在‘粤甜’中的含量积累均显著高于‘巴厘’。由此可见,渗透调节及抗氧化活性、内源激素及代谢物积累的差异是‘粤甜’与‘巴厘’低温响应差异的重要原因。

关键词: 菠萝, 耐寒性, 不同品种, 植物激素

Abstract:

The aim of this trial is to explore the relevant mechanisms of two different pineapple cultivars responding variously to low temperature stress. Having the pineapple cultivars ‘Yuetian’ with stronger chilling-resistance and ‘Comte de Paris’ with weaker chilling-resistance as materials, the physiological aspects, relative expressions of genes and differentially accumulated metabolites(DAMs)of the pineapple leaves were determined and compared under low temperature(LT, 5℃)and normal temperature(NT, 25℃)treatments for 48 h. The obtained results indicated that the contents of soluble protein, soluble sugar and proline, as well as the activities of POD, SOD and CAT enzymes in ‘Yuetian’ were significantly higher than those in ‘Comte de Paris’ after LT treatment, while the content of MDA was significantly lower than that in ‘Comte de Paris’, respectively. After LT treatment, the relative expressions of POD and CAT genes in ‘Yuetian’ were significantly higher than those in ‘Comte de Paris’, respectively; whereas MDA content was significantly lower than that of ‘Comte de Paris’, and the relative expressions of POD and CAT genes was significantly higher than that of ‘Comte de Paris’. The contents of phytohormones of auxin, cytokinin, jasmonic acid, salicylic acid and strigolactone in ‘Yuetian’ were higher than those in ‘Comte de Paris’, nevertheless the contents of gibberellin and abscisic acid were lower than those in ‘Comte de Paris’ after LT treatment, respectively. Metabolomics analysis showed that a total of 262 DAMs were detected between ‘Yuetian’ and ‘Comte de Paris’ after LT treatment, which predominantly included flavonoid metabolites. KEGG analysis indicated that the DAMs were principally enriched in the three pathways including biosynthesis of secondary metabolites, flavonoid biosynthesis, as well as flavone and flavonol biosynthesis. Further, 110 DAMs were screened between ‘Yuetian’ and ‘Comte de Paris’ e induced by LT treatment, and the accumulating levels of these screened metabolites in ‘Yuetian’ were correspondingly observed to be significantly higher than those in ‘Comte de Paris’. Among the 110 DAMs, 41 had significant differences at NT condition, and the fold changes increased after LT treatment. Besides, 69 had no significant differences at NT condition and had significant differences after LT treatment. Summarily, the diversity in osmotic adjustment and anti-oxidized activity, as well as endogenous hormones and metabolites accumulation might be the vital aspects that result in the various responses to low temperature stress for the two involved pineapple cultivars.

Key words: pineapple(Ananas comosus), chilling-resistance, different cultivars, phytohormones