适用于农杆菌侵染的玉米受体材料筛选

生物技术通报 ›› 2012, Vol. 0 ›› Issue (12): 82-87.

适用于农杆菌侵染的玉米受体材料筛选

蒋惠泉^{1, 2}, 任雯¹, 徐游², 张志方¹, 陈浩¹, 贺浩华², 赵久然¹, 刘亚¹

1 北京市农林科学院玉米研究中心，北京 100097 ；2 江西农业大学理学院，南昌 330000

收稿日期:2012-05-05 修回日期:2013-01-25 出版日期:2012-12-26 发布日期:2013-02-06
作者简介:蒋惠泉, 男, 硕士, 研究方向玉米基因工程；E-mail:jhq329@gmail.com
基金资助:
：北京市农林科学院科技创新能力建设专项（KJCX201102003），北京市科技新星项目（Z111105054511066）

Screening on Recipient Material for Agrobacterium-mediatedTransformation in Maize

Jiang Huiquan^1,2, Ren Wen¹, Xu You², Zhang Zhifang¹, Chen Hao¹, He Haohua², Zhao Jiuran¹, Liu Ya¹

1Maize Research Center，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100097 ；2College of Science，Jiangxi AgricultureUniversity，Nanchang 330000

Received:2012-05-05 Revised:2013-01-25 Published:2012-12-26 Online:2013-02-06

摘要/Abstract

摘要： 以培育的14 个玉米新系为材料，对幼胚的Ⅱ型愈伤产率和愈伤再生率，以及幼胚和农杆菌共培养后幼胚的GUS 瞬时表达率进行统计，据此判断不同自交系在组织培养中的潜力，及其是否为农杆菌的易侵染材料，最终试验选出了zpm5 和zpm6两种既适合组织培养又对农杆菌敏感的基因型材料。在对成熟胚愈伤诱导率的统计中发现，成熟胚愈伤诱导率可以作为对幼胚愈伤诱导的预测手段，两者的愈伤诱导率呈正相关性。

关键词: 农杆菌, 玉米, 愈伤组织, GUS, 染色

Abstract: At present, Agrobacterium mediated genetic transformation in maize is widely used in the world. A good recipient material isvery important for the transformation system. In this study, 14 new inbreed lines were used to choose the suitable recipient material by analysingembryogenic Type Ⅱ callus induction, plant regeneration rate and GUS transient expression. Zpm2 and zpm5 were finally screened for maizetransformation system. In the study of mature embryos callus induction, mature embryos callus induction rate can predict the growth status ofimmature embryos callus and their callus induction show a positive correlation.

蒋惠泉, 任雯, 徐游, 张志方, 陈浩, 贺浩华, 赵久然, 刘亚. 适用于农杆菌侵染的玉米受体材料筛选[J]. 生物技术通报, 2012, 0(12): 82-87.

Jiang Huiquan, Ren Wen, Xu You, Zhang Zhifang, Chen Hao, He Haohua, Zhao Jiuran, Liu Ya. Screening on Recipient Material for Agrobacterium-mediatedTransformation in Maize[J]. Biotechnology Bulletin, 2012, 0(12): 82-87.

参考文献

[1] Dai S, Zheng P, Marmey P, et al. Comparative analysis of transgenicrice plants obtained by Agrobacterium-mediated transformation andparticle bombardment. Mol Breed, 2011, 7(1)：25-33.
[2] Birch RG. Plant transformation：problems and strategies for practicalapplication. Annu Rev Plant Physiol Plant Mol Biol, 1997, 48: 297-326.
[3] Hiei Y, Komari T, Ishida Y, Saito H. Development of Agrobacteriummediatedtransformation method for mono-cotyledonous plants. Breed,2000, 2: 205-213.
[4] Cheng M, Fry JE, Pang S, et al. Genetic transformation of wheatmediated by Agrobacterium tumefaciens. Plant Physiol, 1997, 115:971-980.
[5] Hiei Y, Ohta S, Komari T, Kumasho T. Efficient transformation ofrice(Oryza sativa L.)mediated by Agrobacterium and sequenceanalysis of the boundaries of the T-DNA. Plant J, 1997, 6(2)：271-282.
[6] Ahloowalia BS. Plant regeneration from callus culture in wheat. CropScience, 1982, 22: 405-410.
[7] Spencer TM, Gordon-Kamm WJ, Daincs RJ, et al. Biolaphosselection of stable transformants from maize cell culture. Theor ApplGenet, 1990, 79: 625-631.
[8] Spencer TM, Gordon-Kamm WJ, Daines RJ. Bialaphos selection ofstable transformation from maize cell culture. Theor Appl Genet,1990, 79: 625-631.
[9] Schlappi M, Hohn B. Competence of immature maize embryos forAgrobacterium-mediated gene. Plant Cell, 1992, 4: 7-16.
[10] Ishida Y, Saito H, Ohta S, et al. High efficiency transformation ofmaize(Zea mays L.)mediated by Agrobacterium tumefaciens. NatBiotechnol, 1996, 14: 745-750.
[11] Zhao Z, Gu W, Cai T, et al. High throughput genetic transformationmediated by Agrobacterium tumefaciens in maize. Molecular Breeding,2001, 8: 323-333.
[12] Frame BR, Shou H, Chikwamba RK, et al. Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binaryvector system. Plant Physiol, 2002, 129(1)：13-22.
[13] Gutlitz RGH, Lamb PW, Matthsse AG. Involvement of carrot cellsurface proteins in attachment of Agrobacterium tumefaciens. PlantPhysiol, 1987, 83(3)：564-575.
[14] Wojtaszek P. Oxidative burst: an early plant response to pathogeninfection. Biochem J, 1997, 322(3)：681-692.
[15] Pollock K, Barfield DG, Shields R. The toxicity of antibiotics toplant cell cultures. Plant Cell Rep, 1983, 2: 36-39.
[16] Hansen G. Evidence for Agrobacterium-induced apoptosis in maizecells. Mol Plant-Microbe Interact, 2000, 13: 649-657.
[17] Green CE, Phillips RL, Kleese RA. Tissue culture of maize(Zeamays L.)：Initiation, maintenance, and organic growth factors,Crop Science, 1974, 14(1)：54-58.
[18] Wang AS. Callus induction and plant regeneration from maizemature embryos. Plant Cell, 1987, 6(5)：360-362.
[19] Huang XQ, Wei ZM. High frequency plant regeneration throuthcallus initiation from mature embryos of maize(Zea mays L.).Plant Cell Rep, 2004, 22(11)：793-800.
[20] 曹俊梅, 窦秉德, 陈莉, 等. 玉米幼胚和成熟胚愈伤组织分化反应性比较. 新疆农业大学学报, 2005, 28(2)：10-13.
[21] Sidorov V, Gilbertson L, Addae P, Duncna D. Agrobacteriummediated transformation of seedling derived maize callus. Plant CellRep, 2006, 25: 320-328.
[22] 王世玉, 郑用琏, 刘亚, 等. 玉米成熟胚胚性愈伤组织的诱导、高频再生及转化的研究. 作物学报, 2008, 34(3)：423-428.
[23] Jefferson RA. Assaying chimeric in plants: the GUS gene fusionsystem. Plant Mol Biol Rep, 1987, 5: 387-405.
[24] Huang X, Wei Z. Successful Agrobacterium-mediated genetictransformation of maize elite inbred lines. Plant Cell Rep, 2005,83: 187-200.