山羊磷脂氢谷胱甘肽过氧化物酶生物信息学分析

生物技术通报 ›› 2012, Vol. 0 ›› Issue (12): 106-113.

山羊磷脂氢谷胱甘肽过氧化物酶生物信息学分析

施力光^{1, 2}, 荀文娟¹, 周汉林¹, 侯冠彧¹, 岳文斌², 张春香², 杨茹洁³

1 中国热带农业科学院热带作物品种资源研究所，儋州 571737 ；2 山西农业大学，太谷 030801 ；3 山西农业大学太原畜牧兽医学院，太原 030024

收稿日期:2012-04-26 修回日期:2013-01-25 出版日期:2012-12-26 发布日期:2013-02-06
作者简介:施力光, 男, 博士, 助理研究员, 研究方向:动物繁殖调控技术;E-mail:shiliguang123@126.com
基金资助:
: 中央级公益性科研院所基本科研业务费专项资金项目（1630032012022）

Bioinformatis Analysis of PHGPx Gene in Goat Capra hircus

Shi Liguang^1,2, Xun Wenjuan², Zhou Hanlin¹, Hou Guanyu¹, Yue Wenbin², Zhang Chunxiang², Yang Rujie³

1Tropical Crops Genetic Resources Institute，Chinese Academy of Tropical Agricultural Sciences，Danzhou 571737 ；2Shanxi AgriculturalUniversity，Taigu 030801 ；3College of Animal Science and Veterinary Medicine，Taiyuan 030024

Received:2012-04-26 Revised:2013-01-25 Published:2012-12-26 Online:2013-02-06

摘要/Abstract

摘要： 旨在克隆山羊磷脂氢谷胱甘肽过氧化物酶（PHGPx）基因cDNA 全序列并进行序列分析。提取山羊睾丸中总RNA，利用RT-PCR 和RACE 技术，扩增山羊PHGPx 基因cDNA 序列并对其生物信息学进行分析。结果表明，山羊PHGPx 基因cDNA 序列全长844 bp，共编码199 个氨基酸；山羊与牛、猪、人和小鼠的氨基酸序列同源性均大于90% ；山羊PHGPx 蛋白二级结构功能区域属谷胱甘肽过氧化物酶家族，预测23-24 和28-29 氨基酸位点有潜在的信号肽位点；UPGMA 算法构建该物种间分子系统进化树，山羊与牛先聚为一类，再分别与猪、鼠、人、鸡聚类，最后与蜜蜂聚类，与物种动物学分类基本吻合。首次克隆了山羊PHGPx 基因，具有GSH-Px 家族典型特征，研究结果将为PHGPx 基因表达分子调控研究提供一定的理论依据。

关键词: 山羊, PHGPx, 基因, RACE, 克隆, 序列分析

Abstract: This study aimed to clone the full length cDNA of goat PHGPx gene. Total RNA was extracted from goat testis and the fulllength cDNA was obtained by the reverse transcription PCR and RACE method. The results demonstrated that the full length of goat PHGPxcDNA was 844 bp, which encode 199 amino acids. The multiple sequence alignments revealed that the identity of goat PHGPx amino acidssequence were above 90% with other species. Bioinformatics analysis indicated that goat PHGPx belonged to GSH-Px family in the secondarystructure, and predicted the signal peptide at the position of 23-24 or 28-29. The molecular phylogenetic trees among species according theUPGMA method indicated that goat and cow assembled, and then assembled with pig, rat, human and chicken, respectively and assembled withbee last. This result of phylogenetic clustering was identical to the zoological classification. In summary, Capra hircus PHGPx gene has beencloned, and it’s concluded that PHGPx has typical characteristics with the homologous genes in GSH-Px family. These results would be thetheoretical foundation for the further study on the genetic control of PHGPx gene expression.

施力光, 荀文娟, 周汉林, 侯冠彧, 岳文斌, 张春香, 杨茹洁. 山羊磷脂氢谷胱甘肽过氧化物酶生物信息学分析[J]. 生物技术通报, 2012, 0(12): 106-113.

Shi Liguang, Xun Wenjuan, Zhou Hanlin, Hou Guanyu, Yue Wenbin, Zhang Chunxiang, Yang Rujie. Bioinformatis Analysis of PHGPx Gene in Goat Capra hircus[J]. Biotechnology Bulletin, 2012, 0(12): 106-113.

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