一株油藏耐热原油降解菌的分离鉴定与特性分析

生物技术通报 ›› 2012, Vol. 0 ›› Issue (12): 156-162.

一株油藏耐热原油降解菌的分离鉴定与特性分析

何华^{1, 2}, 乔发东², 安明理¹, 何蔚荭¹, 杜迅¹, 贾彬¹, 王亚南¹

1 河南省微生物工程重点实验室，郑州 450008 ；2 河南工业大学生物工程学院，郑州 450001

收稿日期:2012-05-29 修回日期:2013-01-25 出版日期:2012-12-26 发布日期:2013-02-06
作者简介:何华, 男, 硕士研究生, 研究方向:环境微生物资源开发与应用;E-mail:hehuabio@yahoo.cn
基金资助:
: 河南省基础与前沿技术研究计划项目（102300413217）, 国家自然科学基金项目（31100582）, 河南省科技攻关计划项目（102102210280）

Isolation，Identification and Characterization of a Crude-oildegradatingThermophiles in Petroleum Reservoirs

He Hua^1,2, Qiao Fadong², An Mingli¹, He Weihong¹, Du Xun¹, Jia Bin¹, Wang Yanan1

1College of Bioengineering，Henan University of Technology，Zhengzhou 450008 ；2Key Laboratory of Microbial Engineering of HenanProvince，Zhengzhou 450001

Received:2012-05-29 Revised:2013-01-25 Published:2012-12-26 Online:2013-02-06

摘要/Abstract

摘要： 从中原油田高温采出液中分离到一株能在55℃高温条件下利用石油烃类生长的耐高温菌株P98-18A1。基于表型、生理生化特性和16S rDNA 序列分析，初步鉴定该菌株为芽孢杆菌属（Bacillus sp.）。通过菌株对原油及部分烷烃降解试验得出，该菌株在适宜条件下，能有效地利用C6-C16 的正构烷烃生长，对链长大于C16 的烷烃在某种程度上具有一定的选择性利用能力。

关键词: 油藏, 耐热微生物, 芽孢杆菌, 石油烃, 生物降解

Abstract: A high temperature-resistant bacteria strain P98-18A1 capable of growing in petroleum hydrocarbons at 55℃ was isolated fromhot produced fluid from Zhongyuan oilfield. This strain was identified as Bacillus sp. based on phenotypic, physiological, biochemical and 16SrDNA sequence analysis. With crude oil and several alkanes degradation experiments, results showed that this strain can effectively utilize C6-C16 n-alkanes, and selectively utilize of the chain longer than C16 alkanes to some extent under the optimum conditions.

何华, 乔发东, 安明理, 何蔚荭, 杜迅, 贾彬, 王亚南. 一株油藏耐热原油降解菌的分离鉴定与特性分析[J]. 生物技术通报, 2012, 0(12): 156-162.

He Hua, Qiao Fadong, An Mingli, He Weihong, Du Xun, Jia Bin, Wang Yanan1. Isolation，Identification and Characterization of a Crude-oildegradatingThermophiles in Petroleum Reservoirs[J]. Biotechnology Bulletin, 2012, 0(12): 156-162.

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