关键词: 家庭冰箱, 细菌, 真菌, 葡萄球菌, 致病

Abstract: It was to understand microbial species and their pathogenic risk in domestic refrigerator. Five domestic refrigerators were randomly selected, from different positions of which bacteria and fungi were isolated using media of nutrition agar（NA）, potato dextrose agar （PDA）and Gause’s No.1. The isolates were identified by 16S rRNA gene, 26S rRNA gene or ITS sequences analysis and morphological characteristics observation using the scanning electron microscopy（SEM）. Total of 84 bacterial and 74 fungal strains were isolated. Most of bacteria belonged to the genera of Bacillus, Staphylococcus, Kocuria and Pseudomonas. Fungal strains mainly belonged to the genera of Cladosporium, Aspergillus and Penicillium. Pathogenic microbes in domestic refrigerators were mainly composed of the strains in Staphylococcus, while others distributed randomly. Variety of bacteria and fungi existed in domestic refrigerators. Domestic refrigerators were mainly contaminated by Staphylococcus. The safe problem of domestic refrigerator should be taken seriously enough.

Key words: Domestic refrigerator, Bacteria Fungi, Staphylococcus, Pathogenic